Gr-1 antibody | RB6-8C5

Rat anti Mouse Gr-1:FITC

Product Type
Monoclonal Antibody
Clone
RB6-8C5
Isotype
IgG2b
Specificity
Gr-1

Product Code Applications Pack Size List Price Your Price Qty
MCA2387F
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F 0.1 mg loader
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Rat anti Mouse Gr-1 antibody, clone RB6-8C5 recognizes the mouse Gr-1 antigen, a ~21–25 kDa GPI anchored cell surface protein bearing a single uPAR/Ly6 domain that belongs to the Ly-6 family of proteins (Lee et al. 2013). Rat anti Mouse Gr-1 antibody, clone RB6-8C5 reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been reported (Fleming et al. 1993). However, other observations dispute the cross-reactivity of clone RB6-8C5 with the Ly-6C protein with the alternative explanation that certain sub-populations of bone marrow cells simultaneously express both Ly-6C and Ly-6G (Nagendra et al. 2007)

The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes.

Rat anti Mouse Gr-1 antibody, clone RB6-8C5 has been used successfully for the depletion of mature neutrophils in vivo (Czuprynski et al 1994, Daley et al. 2008).

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Normal murine bone marrow cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2b Negative Control:FITC MCA6006F F 0.1 mg loader
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Description Rat IgG2b Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
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loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
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Description Mouse Seroblock FcR

References for Gr-1 antibody

  1. Fleming, T.J. et al. (1993) Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.
    J Immunol. 151 (5): 2399-408.
  2. Hestdal, K. et al. (1991) Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells.
    J Immunol. 147 (1): 22-8.
  3. Czuprynski, C.J. et al. (1994) Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection.
    J Immunol. 152 (4): 1836-46.
  4. Sumagin R et al. (2010) LFA-1 and Mac-1 define characteristically different intralumenal crawling and emigration patterns for monocytes and neutrophils in situ.
    J Immunol. 185 (11): 7057-66.
  5. Takano, K. et al. (2011) Successful treatment of acute lung injury with pitavastatin in septic mice: potential role of glucocorticoid receptor expression in alveolar macrophages.
    J Pharmacol Exp Ther. 336: 381-90.
  6. Giroux, M. et al. (2011) SMAD3 prevents graft-versus-host disease by restraining Th1 differentiation and granulocyte-mediated tissue damage.
    Blood.117: 1734-44.
  7. Suttmann, H. et al. (2006) Neutrophil granulocytes are required for effective Bacillus Calmette-Guérin immunotherapy of bladder cancer and orchestrate local immune responses.
    Cancer Res. 66: 8250-7.
  8. Nix, R.N. et al. (2007) Hemophagocytic macrophages harbor Salmonella enterica during persistent infection.
    PLoS Pathog. 3: e193.
  9. View The Latest Product References
  10. Kanda, N. et al. (2011) Visfatin Enhances CXCL8, CXCL10, and CCL20 Production in Human Keratinocytes.
    Endocrinology. 152: 3155-64.
  11. Conlan, J. and North, R. (1994) Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody.
    J Exp Med. 179:259-68.
  12. Takebe, M. et al. (2014) Inhibition of histone deacetylases protects septic mice from lung and splenic apoptosis.
    J Surg Res. 187 (2): 559-70.
  13. Francke, A. et al. (2011) Generation of mature murine monocytes from heterogeneous bone marrow and description of their properties.
    J Histochem Cytochem. 59: 813-25.
  14. Sharp, P.E. et al. (2013) FcγRIIb on myeloid cells and intrinsic renal cells rather than B cells protects from nephrotoxic nephritis.
    J Immunol.190: 340-8.
  15. Hamers, A.A. et al. (2014) Limited role of nuclear receptor Nur77 in Escherichia coli-induced peritonitis.
    Infect Immun. 82 (1): 253-64.
  16. Roche, J.A. et al. (2015) Myofiber damage precedes macrophage infiltration after in vivo injury in dysferlin-deficient a/j mouse skeletal muscle.
    Am J Pathol. 185 (6): 1686-98.
  17. Lee, Y.S. et al. (2015) Interleukin-1 (IL-1) signaling in intestinal stromal cells controls KC/ CXCL1 secretion, which correlates with recruitment of IL-22- secreting neutrophils at early stages of Citrobacter rodentium infection.
    Infect Immun. 83 (8): 3257-67.
  18. Heckelsmiller, K. et al. (2002) Combined dendritic cell- and CpG oligonucleotide-based immune therapy cures large murine tumors that resist chemotherapy.
    Eur J Immunol. 32 (11): 3235-45.
  19. Zhang, M.Z. et al. (2015) Inhibition of cyclooxygenase-2 in hematopoietic cells results in salt-sensitive hypertension.
    J Clin Invest. 125 (11): 4281-94.
  20. Leblond, A.L. et al. (2015) Systemic and Cardiac Depletion of M2 Macrophage through CSF-1R Signaling Inhibition Alters Cardiac Function Post Myocardial Infarction.
    PLoS One. 10 (9): e0137515.
  21. Kojo, K. et al. (2016) BLT1 signalling protects the liver against acetaminophen hepatotoxicity by preventing excessive accumulation of hepatic neutrophils.
    Sci Rep. 6: 29650.
  22. Otsuru, S. et al. (2017) Hematopoietic derived cells do not contribute to osteogenesis as osteoblasts.
    Bone. 94: 1-9.
  23. Wang, Y. et al. (2015) Proximal tubule-derived colony stimulating factor-1 mediates polarization of renal macrophages and dendritic cells, and recovery in acute kidney injury.
    Kidney Int. 88 (6): 1274-1282.
  24. Cousins, F.L. et al. (2016) Evidence for a dynamic role for mononuclear phagocytes during endometrial repair and remodelling.
    Sci Rep. 6: 36748.
  25. Cotrina ML et al. (2017) Direct comparison of microglial dynamics and inflammatory profile in photothrombotic and arterial occlusion evoked stroke.
    Neuroscience. 343: 483-94.
  26. Kamata, M. et al. (2019) Role of the high-affinity leukotriene B4 receptor signaling in fibrosis after unilateral ureteral obstruction in mice.
    PLoS One. 14 (2): e0202842.
  27. Natanov, R. et al. (2018) Blood cytokine expression correlates with early multi-organ damage in a mouse model of moderate hypothermia with circulatory arrest using cardiopulmonary bypass.
    PLoS One. 13 (10): e0205437.
  28. Idowu, T.O. et al. (2020) Identification of specific Tie2 cleavage sites and therapeutic modulation in experimental sepsis.
    Elife. 9: e59520.
  29. Qin, X. et al. (2018) Caspase recruitment domain-containing protein 9 (CARD9) knockout reduces regional ischemia/reperfusion injury through an attenuated inflammatory response.
    PLoS One. 13 (6): e0199711.
  30. Takahashi, R. et al. (2020) Microsomal prostaglandin E synthase-1 promotes lung metastasis via SDF-1/CXCR4-mediated recruitment of CD11b+Gr1+MDSCs from bone marrow.
    Biomed Pharmacother. 121: 109581.
  31. Idowu, T.O. et al. (2021) Flow-dependent regulation of endothelial Tie2 by GATA3 in vivo..
    Intensive Care Med Exp. 9 (1): 38.
  32. Zhang, M.Z. et al. (2019) The Role of the EGF Receptor in Sex Differences in Kidney Injury.
    J Am Soc Nephrol. 30 (9): 1659-73.
  33. Schünke, H. et al. (2021) OTULIN inhibits RIPK1-mediated keratinocyte necroptosis to prevent skin inflammation in mice.
    Nat Commun. 12 (1): 5912.
  34. Konishi, T. et al. (2019) Cell-specific regulatory effects of CXCR2 on cholestatic liver injury.
    Am J Physiol Gastrointest Liver Physiol. 317 (6): G773-G783.
  35. Christodoulou-Vafeiadou, E. et al. (2020) Ectopic bone formation and systemic bone loss in a transmembrane TNF-driven model of human spondyloarthritis.
    Arthritis Res Ther. 22 (1): 232.

Flow Cytometry

Immunofluorescence

Immunohistology - Paraffin

Western Blotting

Synonyms
Ly-6G
UniProt
P35461
Entrez Gene
Ly6g
GO Terms
GO:0009897 external side of plasma membrane
GO:0031225 anchored to membrane
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