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Delta-Like Protein 4 antibody | HMD4-2

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Anti Mouse Delta-Like Protein 4 Antibody, clone HMD4-2 thumbnail image 1 Anti Mouse Delta-Like Protein 4 Antibody, clone HMD4-2 thumbnail image 2
Anti Mouse Delta-Like Protein 4 Antibody, clone HMD4-2 gallery image 1
Mouse Delta like protein 4 transfected cells stained with Hamster anti Mouse Delta Like Protein 4 (MCA5706)
Anti Mouse Delta-Like Protein 4 Antibody, clone HMD4-2 gallery image 2
Mouse Delta like protein 4 transfected cells stained with Hamster anti Mouse Delta Like Protein 4:RPE (MCA5706PE)

Hamster anti Mouse Delta-Like Protein 4:RPE

Product Type
Monoclonal Antibody
Clone
HMD4-2
Isotype
IgG
Specificity
Delta-Like Protein 4

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Product Code Applications Pack Size List Price Your Price Qty
MCA5706PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader

Hamster anti Mouse Delta-Like Protein 4 antibody, clone HMD4-2 recognizes mouse Delta-like protein 4 (DLL4), one of the five major ligands of the Notch signaling pathway, activated through the binding of specific ligands to the Notch receptors Notch 1-4.

DLL4 is expressed by vascular endothelium, and plays a vital role in embryonic vascular development. DLL4 signaling has been shown to play a role in the angiogenesis of clear-cell renal tumors, and pancreatic, bladder and colonic cancer. DLL4 expression in endothelium cells, can be up-regulated by vascular endothelial growth factor (VEGF) and basic-FGF, and by HIF1 alpha, and that blockade of DLL4 inhibits tumor growth by promoting non-productive angiogenesis.

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Recombinant mouse DLL4.
Fusion Partners
Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control:RPE MCA2356PE F 100 Tests/1ml
>
List Price Your Price
Description Hamster (Armenian) IgG Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
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Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
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Description Mouse Seroblock FcR

References for Delta-Like Protein 4 antibody

  1. Sekine, C. et al. (2009) Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands.
    Int Immunol. 21 (3): 295-301.
  2. Yamanda, S. et al. (2009) Role of ephrinB2 in nonproductive angiogenesis induced by Delta-like 4 blockade.
    Blood. 113 (15): 3631-9.
  3. Sekine, C. et al. (2012) Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes.
    Arthritis Res Ther. 14: R45.

Further Reading

  1. Hoyne, G.F. et al. (2001) Notch signalling in the regulation of peripheral immunity.
    Immunol Rev. 182: 215-27.
  2. Iso, T. et al. (2003) Notch signaling in vascular development.
    Arterioscler Thromb Vasc Biol. 23 (4): 543-53.
  3. Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.
    Nat Rev Mol Cell Biol. 7 (9): 678-89.
  4. Hu, X. et al. (2008) Integrated regulation of Toll-like receptor responses by Notch and interferon-gamma pathways.
    Immunity. 29 (5): 691-703.

Flow Cytometry

Functional Assays

Synonyms
DLL4
UniProt
Q9JI71
Entrez Gene
Dll4
GO Terms
GO:0005509 calcium ion binding
GO:0001525 angiogenesis
GO:0016021 integral to membrane
GO:0005112 Notch binding
GO:0007154 cell communication
GO:0007219 Notch signaling pathway
GO:0030154 cell differentiation
GO:0043537 negative regulation of blood vessel endothelial cell migration

MCA5706PE

164523 INN0312R

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