Browsing Modes for Business Users

CLEC2 antibody | 17D9

ISO 9001 Quality Assurance Manufacturing | Print
Anti Mouse CLEC2 Antibody, clone 17D9 thumbnail image 1 Anti Mouse CLEC2 Antibody, clone 17D9 thumbnail image 2 Anti Mouse CLEC2 Antibody, clone 17D9 thumbnail image 3 Anti Mouse CLEC2 Antibody, clone 17D9 thumbnail image 4
Anti Mouse CLEC2 Antibody, clone 17D9 gallery image 1
Figure A. Alexa Fluor 647 conjugated Rat anti Mouse CD41 (MCA2245A647) and FITC conjugated Rat IgG2b isotype control (MCA6006F). Figure B . Alexa Fluor 647 conjugated Rat anti Mouse CD41 (MCA2245A647) and FITC conjugated Rat anti Mouse CLEC2 (MCA5700F). All experiments performed on murine peripheral blood gated on live single platelets, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer
Anti Mouse CLEC2 Antibody, clone 17D9 gallery image 2
Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD31 (MCA2388A488) and Alexa Fluor 647 conjugated Rat IgG2b isotype control (MCA6006A647). Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD31 (MCA2388A488) and Alexa Fluor 647 conjugated Rat anti Mouse CLEC2 (MCA5700A647). All experiments performed on Mouse peripheral blood gated on live single cell platelets, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer
Anti Mouse CLEC2 Antibody, clone 17D9 gallery image 3
Published customer image:
FITC conjugated Rat anti Mouse CLEC2 antibody, clone 17D9 (MCA5700F) used to evaluate CLEC2 expression on platelets by flow cytometry
Image caption:
CLEC-2 is present at the surfaces of peripheral blood platelets, B cells and CD11bhigh Gr-1high cells at steady-state. Flow cytometry staining strategy to identify the haematopoietic cell subsets found in peripheral blood. Platelets and leucocytes were gated based on their small size (low FSC) and expression of CD41; or larger size (higher FSC) and absence of CD41, respectively. DAPIPos dead cells were excluded from the analysis. B and T lymphocytes (Lympho) and myeloid cells were gated based on their relative expression of CD3ε and CD19. Myeloid subsets were further separated based on their expression of CD11b and Gr-1. Cells positive for CLEC-2 are represented on histograms as a percentage of total cell counts in the population.
This analysis shows results from Clec1b+/+ or Clec1b-/- foetal liver reconstituted animals (WT FL or KO FL, respectively) challenged by intraperitoneal injection of 25μg LPS or PBS (Control). CLEC-2 expression was detected using the 17D9 antibody compared to its respective isotype control. This gating strategy was also applied to peripheral blood samples from tamoxifen-treated Clec1bfl/flxRosa26+/creERT2 and Clec1bfl/fl mice and when analysing CLEC-2 expression with INU1.

From: Lowe KL, Navarro-Núñez L, Bénézech C, Nayar S, Kingston BL, Nieswandt B, Barone F, Watson SP, Buckley CD, Desanti GE.
The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.
Eur J Immunol. 2015 Sep;45(9):2484-93.
Anti Mouse CLEC2 Antibody, clone 17D9 gallery image 4
Published customer image:
FITC conjugated Rat anti Mouse CLEC2 antibody, clone 17D9 (MCA5700F) used to evaluate CLEC2 expression on platelet and leukocyte subsets by flow cytometry
Image caption:
Flow cytometry staining strategy to identify the haematopoietic cell subsets found in the spleen. Platelets and leucocytes are gated based on their small size (low FSC) and expression of CD41; or larger size (higher FSC) and absence of CD41, respectively. Leucocyte populations are positively selected with CD45. Myeloid subsets are separated based on their expression of CD11b and Gr-1. B lymphocytes (B Lympho.) are defined as B220pos CD11bneg. Plasmacytoid dendritic cells (p-DC) are defined as B220pos CD11cpos Gr-1pos. CD11cpos B220neg conventional dendritic cells (c-DC) are separated into two subsets based on their CD11b expression level. CLEC-2 expression is detected using the 17D9 antibody compared to its respective isotype control. Cells positive for CLEC-2 are represented on histograms as a percentage of total cell counts in the population.
This analysis shows results from Clec1b+/+ or Clec1b-/- foetal liver reconstituted animals (WT FL or KO FL, respectively) that were challenged by intraperitoneal injection with 25μg LPS or PBS only (Control). This gating strategy was also applied to analyse cells from the MLN.

From: Lowe KL, Navarro-Núñez L, Bénézech C, Nayar S, Kingston BL, Nieswandt B, Barone F, Watson SP, Buckley CD, Desanti GE.
The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.
Eur J Immunol. 2015 Sep;45(9):2484-93.

Rat anti Mouse CLEC2:RPE

Product Type
Monoclonal Antibody
Clone
17D9
Isotype
IgG2b
Specificity
CLEC2

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
MCA5700PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader

Rat anti Mouse CLEC2 antibody, clone 17D9 recognizes mouse C-type lectin domain family 1 member B, also known as CLEC2 or CLEC1B. CLEC2 acts as a receptor for rhodocytin, a platelet-aggregating protein found in snake venom. CLEC2 expression is not restricted to platelets and also acts as an activation receptor on neutrophils.

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
RBL-2H3 cells stably expressing HA-tagged mouse CLEC2.
Fusion Partners
Spleen cells from immunized Wistar rats were fused with cells of the rat Y3 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

Source Reference

  1. Mourão-sá, D. et al. (2011) CLEC-2 signaling via Syk in myeloid cells can regulate inflammatory responses.
    Eur J Immunol. 41 (10): 3040-53.

References for CLEC2 antibody

  1. Mori, J. et al. (2012) Dominant role of the protein-tyrosine phosphatase CD148 in regulating platelet activation relative to protein-tyrosine phosphatase-1B.
    Arterioscler Thromb Vasc Biol. 32 (12): 2956-65.
  2. Lowe, K.L. et al. (2015) The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.
    Eur J Immunol. 45 (9): 2484-93.
  3. Nakamura-Ishizu A et al. (2015) CLEC-2 in megakaryocytes is critical for maintenance of hematopoietic stem cells in the bone marrow.
    J Exp Med. 212(12):2133-46.
  4. Meinders, M. et al. (2016) Repercussion of Megakaryocyte-Specific Gata1 Loss on Megakaryopoiesis and the Hematopoietic Precursor Compartment.
    PLoS One. 11 (5): e0154342.
  5. Hansen, M. et al. (2020) The RNA-Binding Protein ATXN2 is Expressed during Megakaryopoiesis and May Control Timing of Gene Expression.
    Int J Mol Sci. 21 (3): 967.
  6. Kumode, T. et al. (2022) C-type lectin-like receptor 2 specifies a functionally distinct subpopulation within phenotypically defined hematopoietic stem cell population that contribute to emergent megakaryopoiesis.
    Int J Hematol. Feb 01 [Epub ahead of print].

Further Reading

  1. Kerrigan, A.M. et al. (2009) CLEC-2 is a phagocytic activation receptor expressed on murine peripheral blood neutrophils.
    J Immunol. 182 (7): 4150-7.

Synonyms
CLEC1B
UniProt
Q9JL99
Entrez Gene
Clec1b
GO Terms
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
GO:0007166 cell surface receptor linked signaling pathway

MCA5700PE

163516 165950 INN0512R

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

Request a different product with this specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products
Please note