CD86 antibody | PO3
Rat anti Mouse CD86:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- PO3
- Isotype
- IgG2b
- Specificity
- CD86
Antibody Quality and Performance Guarantee
Mouse anti Sheep CD230 (aa151 - aa159) antibody, clone 2G11 (MCA2460) used for the detection of prion protein in scrapie infected sheep by immunohistochemistry.
Image caption:
Photomicrographs of retinal cross-sections of unaffected (a, d) and naturally scrapie-affected (b, c, e) sheep. All layers of the control retina show normal organization and cytoarchitecture (a). Ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL), photoreceptor segments (PS) and choroid (CHOR). Bar: 100 μm. In the scrapie-affected retina, not only was there a disorganization and thickening of the PS layer, but also an overall decrease of cellular density within the INL and ONL (b). Arrowhead indicates displaced photoreceptor nuclei in the PS layer. Bar: 100 μm. Immnohistochemical detection of abnormal PrP deposits (peroxydase-DAB, brown deposits-2G11 monoclonal mouse antibody) in a scrapie retina (c). Asterisks indicate the amorphous material accumulated in the photoreceptor outer segment layer. Bar: 100 μm. Müller cells labelling (GFAP rabbit polyclonal antibody peroxydase-DAB, brown coloration) in the retina from a control (d) and a naturally-scrapie affected sheep (e). Bars: 300μm.
From: Regnier A, Andreoletti O, Albaric O, Gruson DC, Schelcher F, Toutain PL.
Clinical, electroretinographic and histomorphometric evaluation of the retina in sheep with natural scrapie.
BMC Vet Res. 2011 Jun 6;7:25.
doi: 10.1186/1746-6148-7-25.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
F Reset| Rat anti mouse CD86 antibody, clone PO3 recognizes mouse CD86 (B7-2), a ~80 kDa cell surface glycoprotein, a member of the CD28/B7 family. In mouse CD86 is expressed at high levels on peripheral blood monocytes and dendritic cells, and at low levels on resting B and T-lymphocytes. Expression of CD86, on these cell populations, can be increased upon activation. CD86 has been identified, along with CD80 (B7-1), as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA4). CD28 and CTLA4 are two receptors that have opposing functions in T-cell stimulation. Interaction of CD86 with CD28 promotes a number of T-cell activities, whereas the binding of CD86 to CTLA4 inhibits T-cell responses. Rat anti mouse CD86 antibody, clone PO3 has been reported to block some co-stimulatory functions of CD86 (Nakajima et al. 1997). |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Mouse B-cell line, BCL1.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized Sprague Dawley rats were fused with cells of the P3U1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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| Description | Mouse Seroblock FcR | ||||||
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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| Description | Mouse Seroblock FcR | ||||||
References for CD86 antibody
-
Nakajima, A. et al. (1997) Requirement of CD28-CD86 co-stimulation in the interaction between antigen-primed T helper type 2 and B cells.
Int Immunol. 9 (5): 637-44. -
Nozawa, K. et al. (2001) Preferential blockade of CD8(+) T cell responses by administration of anti-CD137 ligand monoclonal antibody results in differential effect on development of murine acute and chronic graft-versus-host diseases.
J Immunol. 167 (9): 4981-6. -
Jin, L.P. et al. (2004) Adoptive transfer of paternal antigen-hyporesponsive T cells induces maternal tolerance to the allogeneic fetus in abortion-prone matings.
J Immunol. 173 (6): 3612-9. -
Bedoret, D. et al. (2009) Lung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice.
J Clin Invest. 119 (12): 3723-38. -
Legutko, A. et al. (2011) Sirtuin 1 Promotes Th2 Responses and Airway Allergy by Repressing Peroxisome Proliferator-Activated Receptor-{gamma} Activity in Dendritic Cells.
J Immunol. 187: 4517-29. -
Lei, Y. et al. (2021) miR-129-5p Ameliorates Ischemic Brain Injury by Binding to SIAH1 and Activating the mTOR Signaling Pathway.
J Mol Neurosci. 71 (9): 1761-71. -
Li, T. et al. (2019) NLRP3 inflammasome-activating arginine-based liposomes promote antigen presentations in dendritic cells.
Int J Nanomedicine. 14: 3503-16. -
Chen, Z. et al. (2023) Vascularized polypeptide hydrogel modulates macrophage polarization for wound healing.
Acta Biomater. 155: 218-34.
View The Latest Product References
-
De Vito, A. et al. (2020) Overexpression of Murine Rnaset2 in a Colon Syngeneic Mouse Carcinoma Model Leads to Rebalance of Intra-Tumor M1/M2 Macrophage Ratio, Activation of T Cells, Delayed Tumor Growth, and Rejection.
Cancers (Basel). 12 (3)Mar 18 [Epub ahead of print]. -
Wang, C. et al. (2024) The Role of TLR7 and TLR9 in the Pathogenesis of Systemic Sclerosis.
Int J Mol Sci. 25 (11): 6133.
- Synonyms
- B7-2
- RRID
- AB_808473
- UniProt
- P42082
- Entrez Gene
- Cd86
- GO Terms
- GO:0002224 toll-like receptor signaling pathway
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0009897 external side of plasma membrane
- GO:0042113 B cell activation
- GO:0043231 intracellular membrane-bounded organelle
MCA2463A647
154821
MCA2463A647T
0215
154822
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