CD80 antibody | RM80
Rat anti Mouse CD80:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- RM80
- Isotype
- IgG2a
- Specificity
- CD80
Rat anti Mouse CD80 antibody, clone RM80 recognizes mouse CD80 (B7-1), a ~60 kDa cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells, and expression may be significantly increased upon B lymphocytes by LPS and by IL-4. CD80 has been identified as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4), two structurally similar molecules expressed on T cells. CD28 and CTLA4 are two receptors that have essential but opposing functions in T-cell stimulation. The Interaction of CD80 with CD28 stimulates and sustains T cell responses, whereas the interaction of CD80 with CTLA4 is reported to inhibit T-cell responses. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin - Immunogen
- BCL1 cells expressing CD80.
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Fusion Partners
- Spleen cells from immunized SD rats were fused with cells of the P3U1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/5 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Rat IgG2a Negative Control:Alexa Fluor® 647 | MCA1212A647 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
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Description | Rat IgG2a Negative Control:Alexa Fluor® 647 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse Seroblock FcR | ||||||
Mouse Seroblock FcR | BUF041B | F | 0.5 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse Seroblock FcR |
Source Reference
-
Nuriya, S. et al. (1996) The differential role of CD86 and CD80 co-stimulatory molecules in the induction and the effector phases of contact hypersensitivity.
Int Immunol. 8 (6): 917-26.
References for CD80 antibody
-
Nakajima, A. et al. (1997) Requirement of CD28-CD86 co-stimulation in the interaction between antigen-primed T helper type 2 and B cells.
Int Immunol. 9 (5): 637-44. -
Nozawa, K. et al. (2001) Preferential blockade of CD8(+) T cell responses by administration of anti-CD137 ligand monoclonal antibody results in differential effect on development of murine acute and chronic graft-versus-host diseases.
J Immunol. 167 (9): 4981-6. -
Jin LP et al. (2004) Adoptive transfer of paternal antigen-hyporesponsive T cells induces maternal tolerance to the allogeneic fetus in abortion-prone matings.
J Immunol. 173 (6): 3612-9. -
Inada, T. et al. (2009) Vaccines using dendritic cells, differentiated with propofol, enhance antitumor immunity in mice.
Immunopharmacol Immunotoxicol. 31 (1): 150-7. -
Bedoret, D. et al. (2009) Lung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice.
J Clin Invest. 119 (12): 3723-38. -
Deng, J. et al. (2010) Dendritic cells loaded with ultrasound-ablated tumour induce in vivo specific antitumour immune responses.
Ultrasound Med Biol. 36 (3): 441-8. -
Legutko, A. et al. (2011) Sirtuin 1 Promotes Th2 Responses and Airway Allergy by Repressing Peroxisome Proliferator-Activated Receptor-{gamma} Activity in Dendritic Cells.
J Immunol. 187: 4517-29. -
He, Y.T. et al. (2016) In vitro generation of cytotoxic T lymphocyte response using dendritic cell immunotherapy in osteosarcoma.
Oncol Lett. 12 (2): 1101-6.
View The Latest Product References
-
Kim, I. et al. (2016) Immunological characterization of de novo and recall alloantibody suppression by CTLA4Ig in a mouse model of allosensitization.
Transpl Immunol. 38: 84-92. -
Curina, G. et al. (2018) Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis. REV1 vaccine produced in bioreactor.
Vet Immunol Immunopathol. 198: 44-53.
- Synonyms
- B7-1
- RRID
- AB_915220
- UniProt
- Q00609
- Entrez Gene
- Cd80
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0009897 external side of plasma membrane
- GO:0031295 T cell costimulation
- GO:0046641 positive regulation of alpha-beta T cell proliferation
MCA2462A647
151602MCA2462A647T
151603If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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