CD71 antibody | 8D3
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|Rat anti Mouse CD71 antibody, clone 8D3 recognizes mouse CD71, a 763 amino acid ~95 kDa single pass type II cell surface transmembrane glycoprotein, otherwise known as the transferrin receptor. CD71 is a major iron-binding protein, which plays a key role in the transport of iron into cells that require it. In mice, CD71 is widely expressed on a variety of cells, including Sertoli cells and cells which form the blood brain barrier (BBB) in the central nervous system.
Rat anti Mouse CD71 antibody, clone 8D3 recognizes native, soluble and denatured forms of murine CD71. Binding of the 8D3 antibody to CD71 does not inhibit the proliferation of cell lines tested, and does not interfere with the uptake of iron into cells.
Rat anti Mouse CD71 antibody, clone 8D3 has been used as a BBB transporter vector in mice and is suitable for studying CD71 expression, and iron uptake into different tissues, in the mouse (Kissel et al. 1988).
- Target Species
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- Mouse transformed endothelioma cell line t.end1.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised Lewis rats were fused with the cells of the mouse NS0 myeloma cell line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106
References for CD71 antibody
Kissel, K. et al. (1998) Immunohistochemical localization of the murine transferrin receptor (TfR) on blood-tissue barriers using a novel anti-TfR monoclonal antibody.
Histochem cell Biol. 110: 63-72.
Lee, H.J. et al. (2000) Targeting rat anti-mouse transferrin receptor monoclonal antibodies through blood-brain barrier in mouse.
J Pharmacol Exp Ther. 292 (3): 1048-52.
Zhang, Y. et al. (2004) Intravenous RNA interference gene therapy targeting the human epidermal growth factor receptor prolongs survival in intracranial brain cancer.
Clin Cancer Res. 10 (11): 3667-77.
Cabezón I et al. (2015) Trafficking of Gold Nanoparticles Coated with the 8D3 Anti-Transferrin Receptor Antibody at the Mouse Blood-Brain Barrier.
Mol Pharm. 12 (11): 4137-45.
Lee, H.J. et al. (2002) Imaging gene expression in the brain in vivo in a transgenic mouse model of Huntington's disease with an antisense radiopharmaceutical and drug-targeting technology.
J Nucl Med. 43 (7): 948-56.
Manich, G. et al. (2013) Study of the transcytosis of an anti-transferrin receptor antibody with a Fab' cargo across the blood-brain barrier in mice.
Eur J Pharm Sci. 49 (4): 556-64.
Sehlin, D. et al. (2016) Antibody-based PET imaging of amyloid beta in mouse models of Alzheimer's disease.
Nat Commun. 7: 10759.
Sehlin, D. et al. (2017) Pharmacokinetics, biodistribution and brain retention of a bispecific antibody-based PET radioligand for imaging of amyloid-β.
Sci Rep. 7 (1): 17254.
View The Latest Product References
Jones AR & Shusta EV (2007) Blood-brain barrier transport of therapeutics via receptor-mediation.
Pharm Res. 24 (9): 1759-71.
Pardridge WM (2007) Blood-brain barrier delivery of protein and non-viral gene therapeutics with molecular Trojan horses.
J Control Release. 122 (3): 345-8.
Boado RJ et al. (2009) Engineering and expression of a chimeric transferrin receptor monoclonal antibody for blood-brain barrier delivery in the mouse.
Biotechnol Bioeng. 102 (4): 1251-8.
- Transferrin Receptor
- Entrez Gene
- GO Terms
- GO:0005886 plasma membrane
- GO:0008233 peptidase activity
- GO:0005381 iron ion transmembrane transporter activity
- GO:0016021 integral to membrane
- GO:0004998 transferrin receptor activity
- GO:0005768 endosome
- GO:0006508 proteolysis
- GO:0006879 cellular iron ion homeostasis
- GO:0006897 endocytosis
- View More GO Terms
- GO:0042470 melanosome
- GO:0048471 perinuclear region of cytoplasm
- GO:0070062 extracellular vesicular exosome
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