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CD71 antibody | 8D3

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Anti Mouse CD71 Antibody, clone 8D3 thumbnail image 1 Anti Mouse CD71 Antibody, clone 8D3 thumbnail image 2
Anti Mouse CD71 Antibody, clone 8D3 gallery image 1
Figure A. RPE conjugated rat anti mouse TER-119 (MCA6044PE) and FITC conjugated Rat IgG2a isotype control (MCA1212F) Figure B. RPE conjugated rat anti mouse TER-119 (MCA6044PE) and FITC conjugated rat anti mouse CD71 (MCA2474F). All experiments performed on mouse bone marrow. Data acquired on the ZE5™ Cell Analyzer
Anti Mouse CD71 Antibody, clone 8D3 gallery image 2
Figure A. FITC conjugated rat anti mouse TER-119 (MCA6044F) and RPE conjugated Rat IgG2a isotype control (MCA1212PE) Figure B. FITC conjugated rat anti mouse TER-119 (MCA6044F) and RPE conjugated rat anti mouse CD71 (MCA2474PE). All experiments performed on mouse bone marrow. Data acquired on the ZE5™ Cell Analyzer.

Rat anti Mouse CD71:FITC

Rat anti Mouse CD71:Low Endotoxin

Rat anti Mouse CD71:RPE

Product Type
Monoclonal Antibody
Clone
8D3
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA2474F
Datasheet
F
SDS
0.1 mg loader

MCA2474EL
Datasheet
C F FN
SDS
0.5 mg loader

MCA2474PE
Datasheet
F
SDS
100 Tests loader

Rat anti Mouse CD71 antibody, clone 8D3 recognizes mouse CD71, a 763 amino acid ~95 kDa single pass type II cell surface transmembrane glycoprotein, otherwise known as the transferrin receptor. CD71 is a major iron-binding protein, which plays a key role in the transport of iron into cells that require it. In mice, CD71 is widely expressed on a variety of cells, including Sertoli cells and cells which form the blood brain barrier (BBB) in the central nervous system.

Rat anti Mouse CD71 antibody, clone 8D3 recognizes native, soluble and denatured forms of murine CD71. Binding of the 8D3 antibody to CD71 does not inhibit the proliferation of cell lines tested, and does not interfere with the uptake of iron into cells.

Rat anti Mouse CD71 antibody, clone 8D3 has been used as a BBB transporter vector in mice and is suitable for studying CD71 expression, and iron uptake into different tissues, in the mouse (Kissel et al. 1988).

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Mouse transformed endothelioma cell line t.end1.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Lewis rats were fused with the cells of the mouse NS0 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
Q62351
Entrez Gene
Tfrc
GO Terms
GO:0005886 plasma membrane
GO:0008233 peptidase activity
GO:0005381 iron ion transmembrane transporter activity
GO:0016021 integral to membrane
GO:0004998 transferrin receptor activity
GO:0005768 endosome
GO:0006508 proteolysis
GO:0006879 cellular iron ion homeostasis
GO:0006897 endocytosis
GO:0042470 melanosome
GO:0048471 perinuclear region of cytoplasm
GO:0070062 extracellular vesicular exosome
Regulatory
For research purposes only

Applications of CD71 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/100 1/200
Functional Assays
Immunohistology - Frozen
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106
Flow Cytometry
Use 10ul of the suggested working dilution to label 106
Flow Cytometry
Use 10ul of the suggested working dilution to label 106
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F F 100 Tests loader
Rat IgG2a Negative Control:Low Endotoxin MCA1212EL F 0.5 mg loader
Rat IgG2a Negative Control:RPE MCA1212PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader

Product Specific References

References for CD71 antibody

  1. Kissel, K. et al. (1998) Immunohistochemical localization of the murine transferrin receptor (TfR) on blood-tissue barriers using a novel anti-TfR monoclonal antibody.
    Histochem cell Biol. 110: 63-72.
  2. Lee, H.J. et al. (2000) Targeting rat anti-mouse transferrin receptor monoclonal antibodies through blood-brain barrier in mouse.
    J Pharmacol Exp Ther. 292 (3): 1048-52.
  3. Zhang, Y. et al. (2004) Intravenous RNA interference gene therapy targeting the human epidermal growth factor receptor prolongs survival in intracranial brain cancer.
    Clin Cancer Res. 10 (11): 3667-77.
  4. Cabezón I et al. (2015) Trafficking of Gold Nanoparticles Coated with the 8D3 Anti-Transferrin Receptor Antibody at the Mouse Blood-Brain Barrier.
    Mol Pharm. 12 (11): 4137-45.
  5. Lee, H.J. et al. (2002) Imaging gene expression in the brain in vivo in a transgenic mouse model of Huntington's disease with an antisense radiopharmaceutical and drug-targeting technology.
    J Nucl Med. 43 (7): 948-56.
  6. Manich, G. et al. (2013) Study of the transcytosis of an anti-transferrin receptor antibody with a Fab' cargo across the blood-brain barrier in mice.
    Eur J Pharm Sci. 49 (4): 556-64.
  7. Sehlin, D. et al. (2016) Antibody-based PET imaging of amyloid beta in mouse models of Alzheimer's disease.
    Nat Commun. 7: 10759.

Further Reading

  1. Jones AR & Shusta EV (2007) Blood-brain barrier transport of therapeutics via receptor-mediation.
    Pharm Res. 24 (9): 1759-71.
  2. Pardridge WM (2007) Blood-brain barrier delivery of protein and non-viral gene therapeutics with molecular Trojan horses.
    J Control Release. 122 (3): 345-8.
  3. Boado RJ et al. (2009) Engineering and expression of a chimeric transferrin receptor monoclonal antibody for blood-brain barrier delivery in the mouse.
    Biotechnol Bioeng. 102 (4): 1251-8.

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CD71 resources

Applications

These CD71 antibodies can be used for applications including:

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