Browsing Modes for Business Users

CD69 antibody | H1.2F3

Product Code Applications Pack Size List Price Your Price Qty
MCA1580FT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Hamster anti Mouse CD69 antibody, clone H1.2F3 recognizes the murine CD69 cell surface antigen, an ~85 kDa dimeric glycoprotein. CD69 is expressed rapidly on the surface of T cells, B cells and NK cells following activation.

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Immunogen
Murine dendritic epidermal Y245 cell line.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/BUF041B).

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control:FITC MCA2356F F 0.1 mg loader
>
List Price Your Price
loader
Description Hamster (Armenian) IgG Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

Source Reference

  1. Yokoyama, W.M. et al. (1988) Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation.
    J Immunol. 141 (2): 369-76.

References for CD69 antibody

  1. Iwashiro, M. et al. (2001) Immunosuppression by CD4+ regulatory T cells induced by chronic retroviral infection.
    Proc Natl Acad Sci U S A. 98 (16): 9226-30.
  2. Karrer, U. et al. (2003) Memory inflation: continuous accumulation of antiviral CD8+ T cells over time.
    J Immunol. 170 (4): 2022-9.
  3. Marshall-Clarke, S. et al. (2003) A differential requirement for phosphoinositide 3-kinase reveals two pathways for inducible upregulation of major histocompatibility complex class II molecules and CD86 expression by murine B lymphocytes.
    Immunology. 109 (1): 102-8.
  4. Chalifour, A. et al. (2004) Direct bacterial protein PAMP recognition by human NK cells involves TLRs and triggers alpha-defensin production.
    Blood. 104 (6): 1778-83.
  5. Cohen-sfady, M. et al. (2005) Heat shock protein 60 activates B cells via the TLR4-MyD88 pathway.
    J Immunol. 175 (6): 3594-602.
  6. Hickman, H.D. et al. (2008) Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes.
    Nat Immunol. 9 (2): 155-65.
  7. Kvakan, H. et al. (2009) Regulatory T cells ameliorate angiotensin II-induced cardiac damage.
    Circulation. 119 (22): 2904-12.

Flow Cytometry

Synonyms
AIM
RRID
AB_2075078
UniProt
P37217
Entrez Gene
Cd69
GO Terms
GO:0016021 integral to membrane
GO:0005529 sugar binding
GO:0009897 external side of plasma membrane

MCA1580FT

020614 162960 165233

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with CD69 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products
Please note