CD49b antibody | DX5

Rat anti Mouse CD49b:FITC

Product Type
Monoclonal Antibody
Clone
DX5
Isotype
IgM
Specificity
CD49b

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MCA2125F
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Rat anti Mouse CD49b monoclonal antibody, clone DX5 recognizes mouse CD49b, also known as Very Late Antigen-2 (VLA-2) or alpha-2 integrin. CD49b is a ~160kDa single pass type I membrane protein expressed by NK cells and a small subset of T-cells (Pellicci et al. 2005).

Rat anti mouse CD49b, clone DX5 stains NK cells from all mouse strains tested including Balb/c mice (Arese et al. 2001). CD49b acts as a receptor for collagen, laminin, fibronectin and E-cadherin (Carafoli et al. 2013).

Target Species
Mouse
Product Form
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgM from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
RIL-2 propagated NK1.1+ cells from C57BL/6 mice.
Approx. Protein Concentrations
IgM concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised Lewis rats were fused with the mouse SP2/0 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC only.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).

References for CD49b antibody

  1. Arase, H. et al. (2001) Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2).
    J Immunol. 167 (3): 1141-4.
  2. Jacobs, N. et al. (2006) Intradermal immune response after infection with Vaccinia virus.
    J Gen Virol. 87: 1157-61.
  3. Yin, Y. et al. (2013) Phosphatidylserine-targeting antibody induces M1 macrophage polarization and promotes myeloid-derived suppressor cell differentiation.
    Cancer Immunol Res. 1 (4): 256-68.
  4. Lin, C.Y. et al. (2008) Combined immunogene therapy of IL-6 and IL-15 enhances anti-tumor activity through augmented NK cytotoxicity.
    Cancer Lett. 272: 285-95.
  5. Thomson, R.C. et al. (2008) Expansion and activation of NK cell populations in a gammaherpesvirus infection.
    Scand J Immunol. 67: 489-95.
  6. Pais, R. et al. (2012) An intracameral injection of antigen induces in situ chemokines and cytokines required for the generation of circulating immunoregulatory monocytes.
    PLoS One. 7: e43182.

Flow Cytometry

Synonyms
Integrin Alpha 2 Chain
VLA-2
RRID
AB_323718
UniProt
Q62469
Entrez Gene
Itga2
GO Terms
GO:0007155 cell adhesion
GO:0004872 receptor activity
GO:0007229 integrin-mediated signaling pathway
GO:0009897 external side of plasma membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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