CD49b antibody | DX5
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|Rat anti Mouse CD49b monoclonal antibody, clone DX5 recognizes mouse CD49b, also known as Very Late Antigen-2 (VLA-2) or alpha-2 integrin. CD49b is a ~160kDa single pass type I membrane protein expressed by NK cells and a small subset of T-cells (Pellicci et al. 2005).
Rat anti mouse CD49b, clone DX5 stains NK cells from all mouse strains tested including Balb/c mice (Arese et al. 2001). CD49b acts as a receptor for collagen, laminin, fibronectin and E-cadherin (Carafoli et al. 2013).
- Target Species
- Product Form
- Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgM from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- RIL-2 propagated NK1.1+ cells from C57BL/6 mice.
- Approx. Protein Concentrations
- IgM concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised Lewis rats were fused with the mouse SP2/0 myeloma cell line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
References for CD49b antibody
Arase, H. et al. (2001) Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2).
J Immunol. 167 (3): 1141-4.
Jacobs, N. et al. (2006) Intradermal immune response after infection with Vaccinia virus.
J Gen Virol. 87: 1157-61.
Yin, Y. et al. (2013) Phosphatidylserine-targeting antibody induces M1 macrophage polarization and promotes myeloid-derived suppressor cell differentiation.
Cancer Immunol Res. 1 (4): 256-68.
Lin, C.Y. et al. (2008) Combined immunogene therapy of IL-6 and IL-15 enhances anti-tumor activity through augmented NK cytotoxicity.
Cancer Lett. 272: 285-95.
Thomson, R.C. et al. (2008) Expansion and activation of NK cell populations in a gammaherpesvirus infection.
Scand J Immunol. 67: 489-95.
Pais, R. et al. (2012) An intracameral injection of antigen induces in situ chemokines and cytokines required for the generation of circulating immunoregulatory monocytes.
PLoS One. 7: e43182.
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