CD45 antibody | YW62.3
Rat anti Mouse CD45 antibody, clone YW62.3 (MCA1031GA) used for the detection of microglia in mouse brain by immunohistochemistry on cryostat sections.
Image caption:
Age-related, Tau Induced CD45 activation. Immunohistochemistry was performed for CD45 in rTg4510 and nontransgenic (nTg) littermates aged 1 (A, B, H, I), 5 (C, D, J, K), and 9 (E, F, L, M) months in the anterior cortex (A-F) and hippocampus (H-M). Panels G and N present mean ± S.E.M of % Area for immunostaining of CD45+ microglia in the anterior cortex and hippocampus, respectively. Statistical analysis was performed using 2-way ANOVA followed by Fisher's PLSD multiple comparison test. Area stained for CD45 significantly increased in rTg4510 mice at 9 months of age in the anterior cortex and hippocampus compared with nontransgenic littermates or with younger mice. Lines above relevant bars display significant differences between groups (*p < 0.05), n = 4-5. Sections were digitized and representative images were taken at 40× magnification. Scale bar represents 50 μm.
From: Lee DC, Rizer J, Selenica ML, Reid P, Kraft C, Johnson A, Blair L, Gordon MN, Dickey CA, Morgan D. LPS- induced inflammation exacerbates phospho-tau pathology in rTg4510 mice. J Neuroinflammation. 2010 Sep 16;7:56.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD45 antibody, clone YW62.3 (MCA1031GA) used for the detection of microglia in mouse brain by immunohistochemistry on cryostat sections.
Image caption:
LPS-Induced CD45 Activation in rTg4510 and nontransgenic mice. The microglia marker CD45 was examined by immunohistochemical staining in anterior cortex, hippocampus (CA1), and entorhinal cortex (ECX) after LPS administration. rTg4510 mice or nontransgenic (nTg) littermates were injected unilaterally with LPS or vehicle into the anterior cortex and hippocampus, followed by 1 week survival. Representative images from anterior cortex of nontransgenic (A, C) or rTg4510 mice (B, D) from vehicle- (A, B) or LPS-injected (C, D) mice are shown. Images (F-I) were collected from the CA1 stratum radiatum (CA1) of non-transgenic (F, H) or rTg4510 mice (G, I) after vehicle (F, G) or LPS (H, I) injection. Similar images (K-N) were collected from the entorhinal cortex (ECX) of nontransgenic (K, M) or rTg4510 mice (L, N) after vehicle (K, L) or LPS (M, N) injection. Quantitation of % area containing positive immunostaing is presented for each brain region in E, J, and O (mean ± S.E.M, n=6-8). CD45+ staining increased in both rTg4510 and nontransgenic littermates in anterior cortex, CA1, and ECX of mice treated with LPS compared to vehicle-treated mice. Statistical analysis was performed using 2-way ANOVA followed by Fisher's PLSD multiple comparison test. The asterisk indicates *p < 0.05), n = 6 = 8. Scale bar represents 20 μm.
From: Lee DC, Rizer J, Selenica ML, Reid P, Kraft C, Johnson A, Blair L, Gordon MN, Dickey CA, Morgan D. LPS- induced inflammation exacerbates phospho-tau pathology in rTg4510 mice. J Neuroinflammation. 2010 Sep 16;7:56.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD45 antibody, clone YW62.3 (MCA1031GA) used for the detection of microglia in mouse brain by immunofluorescence on cryostat sections.
Image caption:
Immunofluorescent labeling of microglial activation and phospho-tau in rTg4510 mice treated with LPS. Panels A-C shows immunofluorescent staining of arginase-1 positive microglia (green; A, C) and phospho-tau ser396 (red; B, C) in the anterior cortex. Panels D-F shows YM1 positive microglia (green; D, F) and AT8 (phospho-tauSer202/Thr204) (red; E, F) in the anterior cortex. Panels G-L shows increased CD45 positive microglia (Green; G, I, J, L), phospho-tauSer199/202 (red; H, I), and full-length tau (red; K, L) in the hippocampus CA region. Activated microglia failed to co-label with phospho-tau markers. Images were taken at 20x objective. Scale bar represents 20 μm.
From: Lee DC, Rizer J, Selenica ML, Reid P, Kraft C, Johnson A, Blair L, Gordon MN, Dickey CA, Morgan D.
LPS- induced inflammation exacerbates phospho-tau pathology in rTg4510 mice.
J Neuroinflammation. 2010 Sep 16;7:56.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD45 antibody, clone YW62.3 (MCA1031G) used to label infiltrating leucocytes in murine optic nerve tissue by immunohistochemistry on cryosections.
Image caption:
EAU in the C57BL/6J mouse induces optic nerve head changes detectable on OCT.
Representative images from an eye 15 days post-induction of EAU (D) and matched CFA control (H). Matched histology demonstrates CD45+ infiltration (DAB in brown with haematoxylin counterstain) during EAU (D). With TEFI alone (E), it is possible that changes in disc appearance would erroneously be identified as disease with infiltration in the CFA control, as OCT and immunohistochemistry do not corroborate any evidence of disease.
From: Chu CJ, Herrmann P, Carvalho LS, Liyanage SE, Bainbridge JWB, Ali RR, et al. (2013)
Assessment and In Vivo Scoring of Murine Experimental Autoimmune Uveoretinitis Using Optical Coherence Tomography.
PLoS ONE 8(5): e63002.
doi: 10.1371/journal.pone.0063002
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD45 antibody, clone YW62.3 (MCA1031G) used to label infiltrating leucocytes in murine optic nerve tissue by immunohistochemistry on cryosections.
Image caption:
Abnormal OCT signals in vivo correspond to structural changes and associated cellular infiltrates.
(D) Note the CD45+ cellular infiltrate (DAB in brown with haematoxylin counterstain) around the superficial vessel and under the displaced outer nuclear layer.
From: Chu CJ, Herrmann P, Carvalho LS, Liyanage SE, Bainbridge JWB, Ali RR, et al. (2013)
Assessment and In Vivo Scoring of Murine Experimental Autoimmune Uveoretinitis Using Optical Coherence Tomography.
PLoS ONE 8(5): e63002.
doi: 10.1371/journal.pone.0063002
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD45 antibody, clone YW62.3 (MCA1031G) used to detect CD45 expressing cells by immunofluorescence.
Image caption:
Immune response in tamoxifen-injected iMfn2DA mice.
Representative confocal microscopy images of control and iMfn2DA mouse midbrain 3, 6, and 9 weeks after tamoxifen injection. The brain sections were stained with antibodies against CD45 (red) and TH (blue) (Scale bars: 50 μm).
From: Filograna R, Lee S, Tiklova K, Mennuni M, Jonsson V, Ringnér M, et al. (2021)
Mitochondrial dysfunction in adult midbrain dopamine neurons triggers an early immune response.
PLoS Genet 17(9): e1009822.
doi:10.1371/journal.pgen.1009822
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD45:StarBright UltraViolet 510
- Product Type
- Monoclonal Antibody
- Clone
- YW62.3
- Isotype
- IgG2b
- Specificity
- CD45
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD45 antibody, clone YW62.3 recognizes the murine CD45 cell surface antigen, a single pass type1 transmembrane glycoprotein also known as protein tyrosine phosphatase receptor type C (PTPRC) and originally termed Leucocyte Common Antigen (LCA). CD45 is a 180-220kDa glycoprotein expressed by all leucocytes. CD45 is encoded by 3 alleles in mice, differentially expressed by various inbred strains. The Ly5 gene was originally described with the gene product LY5.1 expressed in C57bl/6 and Ly5.2 expressed in SJL strains (Komura et al. 1975), this was subsequently expanded to include a third allele encoding Ly5.3 (Shen et al. 1986). Further, in 1987 a reversal of nomenclature was instigated resulting in the allele in C57bl/6 becoming Ly5b encoding Ly5.2 and the allele in SJL mice becoming Ly5a encoding Ly5.1 (Morse et al. 1987). Further changes were made in 1992 with Ly5.1 becoming CD45.1 (SJL) and Ly5.2 becoming CD45.2 (C57bl/6). Finally, following work demonstrating homology between the CD45 antigen and a receptor linked protein tyrosine phosphatase the CD45a gene was renamed Ptprca and CD45b renamed Ptprcb (Charbonneau et al. 1988; Zebedee et al. 1991). A number of different isoforms of CD45 are expressed on murine leucocytes depending on the pattern of alternative splicing of 3 exons termed A, B and C encoding regions of ~ 50 amino acids located at the N terminal region of the extracellular portion of CD45. The restricted proteins are termed CD45R with a designation depending on the expressed codon product. (Birkeland et al. 1989). Rat anti mouse CD45 antibody, clone YW62.3 is reactive with all isoforms of murine CD45. N.B. Some reactivity with human tissue has been observed. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to StarBright UltraViolet 510 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Immunogen
- Mouse spleen cells.
- Fusion Partners
- Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright UltraViolet 510 340 513 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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| Description | Mouse Seroblock FcR | ||||||
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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References for CD45 antibody
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Watt, S.M. et al. (1987) Cell-surface markers on haemopoietic precursors. Reagents for the isolation and analysis of progenitor cell subpopulations.
Mol Cell Probes. 1 (4): 297-326. -
Kondo, Y. et al. (2007) Osteopetrotic (op/op) mice have reduced microglia, no Abeta deposition, and no changes in dopaminergic neurons.
J Neuroinflammation. 4: 31. -
Wang, S. et al. (2008) Drak2 contributes to West Nile virus entry into the brain and lethal encephalitis.
J Immunol. 181: 2084-91. -
Chan, D.A. et al (2009) Tumor vasculature is regulated by PHD2-mediated angiogenesis and bone marrow-derived cell recruitment.
Cancer Cell. 15: 527-38. -
Lee, S. et al. (2010) CX3CR1 deficiency alters microglial activation and reduces beta-amyloid deposition in two Alzheimer's disease mouse models.
Am J Pathol. 177: 2549-62. -
Dénes, A. et al. (2010) Chronic systemic infection exacerbates ischemic brain damage via a CCL5 (regulated on activation, normal T-cell expressed and secreted)-mediated proinflammatory response in mice.
J Neurosci. 30: 10086-95. -
Yoshizaki, A. et al. (2010) Cell adhesion molecules regulate fibrotic process via Th1/Th2/Th17 cell balance in a bleomycin-induced scleroderma model.
J Immunol. 185: 2502-15. -
Yang, J. et al. (2010) Evaluation of bone marrow- and brain-derived neural stem cells in therapy of central nervous system autoimmunity.
Am J Pathol. 177: 1989-2001.
View The Latest Product References
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Yang, R. et al. (2010) Successful treatment of experimental glomerulonephritis with IdeS and EndoS, IgG-degrading streptococcal enzymes.
Nephrol Dial Transplant. 25: 2479-86. -
Reed-Geaghan, E.G. et al. (2010) Deletion of CD14 attenuates Alzheimer's disease pathology by influencing the brain's inflammatory milieu.
J Neurosci. 30: 15369-73. -
Paz, H. et al. (2010) The homeobox gene Hhex regulates the earliest stages of definitive hematopoiesis.
Blood. 116: 1254-62. -
Lee, D.C. et al. (2010) LPS- induced inflammation exacerbates phospho-tau pathology in rTg4510 mice.
J Neuroinflammation. 7: 56. -
Lebson, L. et al. (2010) Trafficking CD11b-positive blood cells deliver therapeutic genes to the brain of amyloid-depositing transgenic mice.
J Neurosci. 30: 9651-8. -
Long, G.G. et al. (2010) Hematopoietic Proliferative Lesions in the Spleen of rasH2 Transgenic Mice Treated with MNU.
Toxicol Pathol. 38: 1026-36. -
Drake, C. et al. (2011) Brain inflammation is induced by co-morbidities and risk factors for stroke.
Brain Behav Immun. 25: 1113-22. -
Jawhara, S. et al. (2012) Integrin αXβz is a leukocyte receptor for Candida albicans and is essential for protection against fungal infections.
J Immunol. 189 (5): 2468-77. -
Mills, J.H. et al. (2012) A2A adenosine receptor signaling in lymphocytes and the central nervous system regulates inflammation during experimental autoimmune encephalomyelitis.
J Immunol. 188 (11): 5713-22. -
Zirger, J.M. et al. (2012) Immune-mediated loss of transgene expression from virally transduced brain cells is irreversible, mediated by IFNγ, perforin, and TNFα, and due to the elimination of transduced cells.
Mol Ther. 20 (4): 808-19. -
Yamauchi, S. et al. (2012) Myosin II-dependent exclusion of CD45 from the site of Fcγ receptor activation during phagocytosis.
FEBS Lett. 586: 3229-35. -
Abramowski, D. et al. (2012) Transgenic Expression of Intraneuronal Aβ42 But Not Aβ40 Leads to Cellular Aβ Lesions, Degeneration, and Functional Impairment without Typical Alzheimer's Disease Pathology.
J Neurosci. 32: 1273-83. -
Chu, C.J. et al. (2013) Assessment and in vivo scoring of murine experimental autoimmune uveoretinitis using optical coherence tomography.
PLoS One. 8 (5): e63002. -
Murinello, S. et al. (2014) Fcγ receptor upregulation is associated with immune complex inflammation in the mouse retina and early age-related macular degeneration.
Invest Ophthalmol Vis Sci. 55 (1): 247-58. -
Yazid, S. et al. (2015) Annexin-A1 restricts Th17 cells and attenuates the severity of autoimmune disease.
J Autoimmun. 58: 1-11. -
Benson, C. et al. (2015) Voluntary wheel running delays disease onset and reduces pain hypersensitivity in early experimental autoimmune encephalomyelitis (EAE).
Exp Neurol. 271: 279-90. -
Carbajal, K.S. et al. (2015) Th Cell Diversity in Experimental Autoimmune Encephalomyelitis and Multiple Sclerosis.
J Immunol. 195 (6): 2552-9. -
Kan, M.J. et al. (2015) Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.
J Neurosci. 35 (15): 5969-82. -
Marcos, E. et al. (2016) Dengue encephalitis-associated immunopathology in the mouse model: Implications for vaccine developers and antigens inducer of cellular immune response.
Immunol Lett. 176: 51-6. -
Haile, W.B. et al. (2016) The Janus kinase inhibitor ruxolitinib reduces HIV replication in human macrophages and ameliorates HIV encephalitis in a murine model.
Neurobiol Dis. 92 (Pt B): 137-43. -
Park, S.A. et al. (2016) Deficiency in either COX-1 or COX-2 genes does not affect amyloid beta protein burden in amyloid precursor protein transgenic mice.
Biochem Biophys Res Commun. 478 (1): 286-92. -
Srivastava, A.K. et al. (2016) Co-transplantation of syngeneic mesenchymal stem cells improves survival of allogeneic glial-restricted precursors in mouse brain.
Exp Neurol. 275 Pt 1: 154-61. -
Zhu, C. et al. (2020) Antinociceptive effect of intrathecal injection of miR-9-5p modified mouse bone marrow mesenchymal stem cells on a mouse model of bone cancer pain.
J Neuroinflammation. 17 (1): 85. -
Hargreaves, A. et al. (2021) Tumors modulate fenestrated vascular beds and host endocrine status.
J Appl Toxicol. 41 (12): 1952-65. -
Filograna, R. et al. (2021) Mitochondrial dysfunction in adult midbrain dopamine neurons triggers an early immune response.
PLoS Genet. 17 (9): e1009822. -
Hargreaves, A. et al. (2022) Tumours modulate the systemic vascular response to anti-angiogenic therapy.
J Appl Toxicol. 42 (8): 1371-84. -
Chen, Y.H. et al. (2020) Functionally distinct IFN-γ(+) IL-17A(+) Th cells in experimental autoimmune uveitis: T-cell heterogeneity, migration, and steroid response.
Eur J Immunol. 50 (12): 1941-51. -
Lepland, A. et al. (2024) Therapeutic Tumor Macrophage Reprogramming in Breast Cancer Through a Peptide-Drug Conjugate
bioRχiv: 12 Aug [Epub ahead of print].
- Synonyms
- LCA
- UniProt
- P06800
- Entrez Gene
- Ptprc
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0001915 negative regulation of T cell mediated cytotoxicity
- GO:0001916 positive regulation of T cell mediated cytotoxicity
- GO:0001960 negative regulation of cytokine-mediated signaling pathway
- GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin
- GO:0051726 regulation of cell cycle
- GO:0004725 protein tyrosine phosphatase activity
- GO:0005887 integral to plasma membrane
- GO:0005925 focal adhesion
- View More GO Terms
- GO:0006469 negative regulation of protein kinase activity
- GO:0006470 protein dephosphorylation
- GO:0007159 leukocyte cell-cell adhesion
- GO:0008201 heparin binding
- GO:0009897 external side of plasma membrane
- GO:0019901 protein kinase binding
- GO:0030183 B cell differentiation
- GO:0030890 positive regulation of B cell proliferation
- GO:0031953 negative regulation of protein autophosphorylation
- GO:0034113 heterotypic cell-cell adhesion
- GO:0042098 T cell proliferation
- GO:0042100 B cell proliferation
- GO:0043065 positive regulation of apoptosis
- GO:0043395 heparan sulfate proteoglycan binding
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0045059 positive thymic T cell selection
- GO:0045060 negative thymic T cell selection
- GO:0045121 membrane raft
- GO:0045577 regulation of B cell differentiation
- GO:0045588 positive regulation of gamma-delta T cell differentiation
- GO:0046641 positive regulation of alpha-beta T cell proliferation
- GO:0048304 positive regulation of isotype switching to IgG isotypes
- GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
- GO:0050852 T cell receptor signaling pathway
- GO:0050853 B cell receptor signaling pathway
- GO:0050855 regulation of B cell receptor signaling pathway
- GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
- GO:0051209 release of sequestered calcium ion into cytosol
- GO:0051607 defense response to virus
MCA1031SBUV510
100006294
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