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CD44 antibody | IM7

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Rat anti Mouse CD44:RPE

Product Type
Monoclonal Antibody
Clone
IM7
Isotype
IgG2b
Specificity
CD44

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MCA4703PE
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Rat anti Mouse CD44 antibody, clone IM7 recognizes CD44, also known as H-CAM, HUTCH and lymphocyte antigen 24 (Ly-24).

CD44 is a cell surface receptor for hyaluronic acid, although it can also interact with other ligands such as collagens and matrix metalloproteinases. This protein plays a role in a variety of cellular functions, such as adhesion, lymphocyte activation and lymph node homing. It is also involved in cell migration and plays a role in tumor metastasis. CD44 has many distinct isoforms responsible for its functional diversity.

Rat anti Mouse CD44 antibody, clone IM7 recognizes all isoforms of CD44 and has been reported to inhibit a delayed-type hypersensitivity response in vivo and to induce complement-mediated cytotoxicity.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Baboon
Cynomolgus monkey
Rhesus Monkey
Horse
Bovine
Pig
Dog
Cat
Ferret
Mustelid Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells.
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for CD44 antibody

  1. Lesley, J. et al. (1992) Requirements for hyaluronic acid binding by CD44: a role for the cytoplasmic domain and activation by antibody.
    J Exp Med. 175: 257-66.
  2. Camp, R.L. et al. (1993) CD44 is necessary for optimal contact allergic responses but is not required for normal leukocyte extravasation.
    J Exp Med. 178: 497-507.
  3. Brocke, S. et al. (1999) Antibodies to CD44 and integrin alpha4, but not L-selectin, prevent central nervous system inflammation and experimental encephalomyelitis by blocking secondary leukocyte recruitment.
    Proc Natl Acad Sci U S A. 96: 6896-901.
  4. Morrison, H. et al. (2001) The NF2 tumor suppressor gene product, merlin, mediates contact inhibition of growth through interactions with CD44.
    Genes Dev.15: 968-80.
  5. Mckallip, R.J. et al. (2002) Role of CD44 in activation-induced cell death: CD44-deficient mice exhibit enhanced T cell response to conventional and superantigens.
    Int Immunol. 14 (9): 1015-26.
  6. Lesley, J. et al. (2003) Hyaluronan binding by cell surface CD44.
    J Biol Chem. 275: 26967-75.
  7. Katoh, S. et al. (2003) A role for CD44 in an antigen-induced murine model of pulmonary eosinophilia.
    J Clin Invest. 111: 1563-70.
  8. Rutigliano, J.A. et al. (2008) Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection.
    J Immunol Methods. 336: 71-7.
    9. View The Latest Product References
  9. Ariyoshi, W. et al. (2010) Internalization of aggrecan G1 domain neoepitope ITEGE in chondrocytes requires CD44.
    J Biol Chem. 285: 36216-24.
  10. Martín-Villar, E. et al. (2010) Podoplanin associates with CD44 to promote directional cell migration.
    Mol Biol Cell. 21: 4387-99.
  11. Hartley, A.N. & Tarleton, R.L. (2015) Chemokine receptor 7 (CCR7)-expression and IFNγ production define vaccine-specific canine T-cell subsets.
    Vet Immunol Immunopathol. 164 (3-4): 127-36.
  12. Zhu, C. et al. (2020) Antinociceptive effect of intrathecal injection of miR-9-5p modified mouse bone marrow mesenchymal stem cells on a mouse model of bone cancer pain.
    J Neuroinflammation. 17 (1): 85.
  13. Maeda, S. et al. (2021) NAFLD exacerbates cholangitis and promotes cholangiocellular carcinoma in mice.
    Cancer Sci. 112 (4): 1471-80.
  14. Lara, M.L. et al. (2023) Influence of culture conditions on the secretome of mesenchymal stem cells derived from feline adipose tissue: Proteomics approach.
    Biochimie. 211: 78-86.

Flow Cytometry

Functional Assays

Immunohistology - Paraffin

Western Blotting

Synonyms
H-CAM
PGP-1
UniProt
P15379
Entrez Gene
Cd44

MCA4703PE

151208 163096 165382 165804

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