CD44 antibody | 5035-41.1D
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F ResetMouse anti Mouse CD44:FITC
- Product Type
- Monoclonal Antibody
- Clone
- 5035-41.1D
- Isotype
- IgG1
- Specificity
- CD44
Antibody Quality and Performance Guarantee
| Mouse anti Mouse CD44 antibody, clone 5035-41.1D recognizes human CD44, also known as Lymphocyte antigen 24, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Phagocytic glycoprotein I (PGP-1), Hermes antigen or Hyaluronate receptor. CD44 is found on >90% bone marrow cells and spleen cells, 56% of thymus cells and 90% of lymph node cells. Mouse anti Mouse CD44 antibody, clone 5035-41.1D recognizes the following strains of mice:- C57BL/6, C57BL/10, C57/L, C58A, AKR, 129, SJL, NZB, C3H, CE, CBA/H. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- MCA1014F: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- MCA1014FB, MCA1014FT: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Spleen cells from B6PL-Ly-2a / Ly-3a B6-Ly-1a mice.
- Approx. Protein Concentrations
- Pack Size: 0.1 mg, 25 µgIgG concentration 0.1mg/mlPack Size: 0.5 mgIgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse P3-NS1-1-Ag4 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | Neat Pack Size: 0.1 mg, 25 µg 1/50 Pack Size: 0.5 mg |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse Seroblock FcR | ||||||
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse Seroblock FcR | ||||||
Source Reference
-
Sutton, V.R. et al. (1987) Genetic and biochemical characterization of antigens encoded by the Ly-24 (Pgp-1) locus.
J Immunogenet. 14 (1): 43-57.
References for CD44 antibody
-
A, A. et al. (2001) Cell surface-expressed moesin-like receptor regulates T cell interactions with tissue components and binds an adhesion-modulating IL-2 peptide generated by elastase.
J Immunol. 166 (5): 3052-60. -
Qian, K. et al. (2015) Effects of Tanshinone IIA on osteogenic differentiation of mouse bone marrow mesenchymal stem cells.
Naunyn Schmiedebergs Arch Pharmacol. 388 (11): 1201-9.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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