CD321 antibody | H202-106
Rat anti Mouse CD321:Biotin
- Product Type
- Monoclonal Antibody
- Clone
- H202-106
- Isotype
- IgG1
- Specificity
- CD321
Rat anti Mouse CD321 antibody, clone H202-106 recognizes murine CD321, also known as junctional adhesion molecule 1 (JAM-1). CD321 is a 274 amino acid ~32-4 1kDa single pass, type I transmembrane glycoprotein, which shares similarities with related proteins JAM-2 and JAM-3. CD321 is a multifunctional protein primarily expressed by platelets, endothelial and epithelial cells. The CD321 protein co-localises with tight junction molecules in both epithelial and endothelial cells and plays an important role in the regulation of junctional integrity and permeability. In addition, CD321 is a ligand for the integrin LFA-1 and is also involved in the transmigration of leucocytes. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to biotin - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- MTE1/MTE2 stromal cell lines.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Cells from immunized Lou rats were fused with cells of the X63 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/5 |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse Seroblock FcR | ||||||
Mouse Seroblock FcR | BUF041B | F | 0.5 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse Seroblock FcR |
References for CD321 antibody
-
Malergue, F. et al. (1998) A novel immunoglobulin superfamily junctional molecule expressed by antigen presenting cells, endothelial cells and platelets.
Mol Immunol. 35 (17): 1111-9. -
Ebnet, K. et al. (2000) Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1.
J Biol Chem. 275 (36): 27979-88. -
Aurrand-lions, M. et al. (2001) Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members.
Blood. 98 (13): 3699-707. -
Prestwich, R.J. et al. (2009) Immune-mediated antitumor activity of reovirus is required for therapy and is independent of direct viral oncolysis and replication.
Clin Cancer Res. 15 (13): 4374-4381. -
Morita, Y. et al. (2010) Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment.
J Exp Med. 207 (6): 1173-82. -
Schmitt, M.M. et al. (2014) Endothelial junctional adhesion molecule-a guides monocytes into flow-dependent predilection sites of atherosclerosis.
Circulation. 129 (1): 66-76. -
Narni-Mancinelli, E. et al.. (2017) Complement factor P is a ligand for the natural killer cell-activating receptor NKp46.
Sci Immunol; 2(10): eaam9628. -
Nagatake, T. et al. (2020) Selective expression of claudin-5 in thymic endothelial cells regulates the blood-thymus barrier and T-cell export.
Int Immunol. Oct 10 dxaa069 [Epub ahead of print].
View The Latest Product References
- Synonyms
- JAM-1
- RRID
- AB_616673
- UniProt
- O88792
- Entrez Gene
- F11r
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005923 tight junction
- GO:0030855 epithelial cell differentiation
- GO:0044419 interspecies interaction between organisms
MCA2346B
0515 164290If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Mouse ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up