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CD29 antibody | HM beta 1-1

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Anti Mouse CD29 Antibody, clone HM beta 1-1 thumbnail image 1
Anti Mouse CD29 Antibody, clone HM beta 1-1 gallery image 1
Figure A. RPE conjugated rat anti mouse CD3 (MCA500PE) and FITC conjugated hamster IgG isotype control (MCA2356F). Figure B. RPE conjugated rat anti mouse CD3 (MCA500PE) and FITC conjugated hamster anti mouse CD29 (MCA2298F). All experiments performed on red cell lysed murine peripheral blood gated on lymphocytes in the presence of murine SeroBlock (BUF041A).

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Hamster anti Mouse CD29:FITC

Product Type
Monoclonal Antibody
Clone
HM beta 1-1
Isotype
IgG
Specificity
CD29

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Product Code Applications Pack Size List Price Your Price Qty
MCA2298F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
MCA2298FT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 25 µg loader
List Price Your Price
loader

Hamster anti Mouse CD29 antibody, clone HM beta 1-1 recognizes the murine integrin beta 1 subunit (CD29), a ~110 kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. The ligands for these heterodimers include collagen, fibronectin, laminin and vascular adhesion molecule-1. In the immune system beta 1 integrins play an important role in cell adhesion, migration, activation and differentiation.

Hamster anti Mouse CD29 antibody, clone HM beta 1-1 is reported to inhibit beta 1 integrin mediated adhesion (Noto et al. 1995).

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Purified mouse VLA-4 antigen.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized Armenian hamsters were fused with cells of the P3U1 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control:FITC MCA2356F F 0.1 mg loader
>
List Price Your Price
loader
Description Hamster (Armenian) IgG Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

Source Reference

  1. Noto, K. et al. (1995) Identification and functional characterization of mouse CD29 with a mAb.
    Int Immunol. 7 (5): 835-42.

References for CD29 antibody

  1. Eto, D.S. et al. (2007) Integrin-mediated host cell invasion by type 1-piliated uropathogenic Escherichia coli.
    PLoS Pathog. 3: e100.
  2. Kouros-Mehr H (2008) GATA-3 links tumor differentiation and dissemination in a luminal breast cancer model.
    Cancer Cell. 13: 141-52.
  3. Tiede, B.J. et al. (2009) A novel mouse model for non-invasive single marker tracking of mammary stem cells in vivo reveals stem cell dynamics throughout pregnancy.
    PLoS One. 4 (11): e8035.
  4. Li S et al. (2010) Upregulation of CXCR4 favoring neural-like cells migration via AKT activation.
    Neurosci Res. 67 (4): 293-9.
  5. Lu, T.Y. et al. (2010) Epithelial cell adhesion molecule regulation is associated with the maintenance of the undifferentiated phenotype of human embryonic stem cells.
    J Biol Chem. 285: 8719-32.
  6. Xu, L. et al. (2017) Umbilical cord-derived mesenchymal stem cells on scaffolds facilitate collagen degradation via upregulation of MMP-9 in rat uterine scars.
    Stem Cell Res Ther. 8 (1): 84.
  7. Hou, B. et al. (2018) Comparison of the Effects of BMSC-derived Schwann Cells and Autologous Schwann Cells on Remyelination Using a Rat Sciatic Nerve Defect Model.
    Int J Biol Sci. 14 (13): 1910-1922.
  8. Porwal, K. et al. (2019) Increased bone marrow-specific adipogenesis by clofazimine causes impaired fracture healing, osteopenia and osteonecrosis without extra-skeletal effects in rats.
    Toxicol Sci. kfz172.
    9. View The Latest Product References
  9. Zhu, C. et al. (2020) Antinociceptive effect of intrathecal injection of miR-9-5p modified mouse bone marrow mesenchymal stem cells on a mouse model of bone cancer pain.
    J Neuroinflammation. 17 (1): 85.
  10. Farahat, M. et al. (2022) Mechanotransductive Mechanisms of Biomimetic Hydrogel Cues Modulating Meckel's Cartilage Degeneration.
    Adv Biol (Weinh). 2022: e2101315.

Flow Cytometry

Immunofluorescence

Synonyms
Integrin Beta 1 Chain
RRID
AB_566688
UniProt
P09055
Entrez Gene
Itgb1
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0001701 in utero embryonic development
GO:0001708 cell fate specification
GO:0051726 regulation of cell cycle
GO:0004872 receptor activity
GO:0005178 integrin binding
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0008284 positive regulation of cell proliferation
GO:0008354 germ cell migration
GO:0019717 synaptosome
GO:0031594 neuromuscular junction
GO:0042383 sarcolemma
GO:0042470 melanosome
GO:0045214 sarcomere organization
GO:0045596 negative regulation of cell differentiation
GO:0055007 cardiac muscle cell differentiation

MCA2298F

0812 159869

MCA2298FT

154383

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