CD29 antibody | HM beta 1-1
Hamster anti Mouse CD29:Alexa Fluor® 488
Hamster anti Mouse CD29:Alexa Fluor® 647
Hamster anti Mouse CD29:FITC
Hamster anti Mouse CD29:Low Endotoxin
Hamster anti Mouse CD29
Hamster anti Mouse CD29:RPE
- Product Type
- Monoclonal Antibody
- Clone
- HM beta 1-1
- Isotype
- IgG
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA2298A488 | F | 100 Tests/1ml |
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| MCA2298A488T | F | 25 Tests/0.25ml |
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| MCA2298A647 | F | 100 Tests/1ml |
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| MCA2298A647T | F | 25 Tests/0.25ml |
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| MCA2298F | F | 0.1 mg |
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| MCA2298FB | F | 0.5 mg |
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| MCA2298FT | F | 25 µg |
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| MCA2298EL | F FN IP | 0.5 mg |
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| MCA2298GA | F IP | 0.1 mg |
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| MCA2298PE | F | 100 Tests |
|
Hamster anti Mouse CD29 antibody, clone HM beta 1-1 recognizes the murine integrin beta 1 subunit (CD29), a ~110 kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. The ligands for these heterodimers include collagen, fibronectin, laminin and vascular adhesion molecule-1. In the immune system beta 1 integrins play an important role in cell adhesion, migration, activation and differentiation.
Hamster anti Mouse CD29 antibody, clone HM beta 1-1 is reported to inhibit beta 1 integrin mediated adhesion (Noto et al. 1995).
Hamster anti Mouse CD29 antibody, clone HM beta 1-1 is reported to inhibit beta 1 integrin mediated adhesion (Noto et al. 1995).
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - conjugated to Alexa Fluor®488 - liquid
- Product Form
- Purified IgG - conjugated to Alexa Fluor®647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Purified mouse VLA-4 antigen.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- Pack Size: 0.1 mg, 25 µgIgG concentration 0.1 mg/mlPack Size: 0.5 mgIgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Armenian hamsters were fused with cells of the P3U1 mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P09055
- Entrez Gene
- Itgb1
- GO Terms
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0001701 in utero embryonic development
- GO:0001708 cell fate specification
- GO:0051726 regulation of cell cycle
- GO:0004872 receptor activity
- GO:0005178 integrin binding
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0008284 positive regulation of cell proliferation
- GO:0008354 germ cell migration
- GO:0019717 synaptosome
- GO:0031594 neuromuscular junction
- GO:0042383 sarcolemma
- GO:0042470 melanosome
- GO:0045214 sarcomere organization
- GO:0045596 negative regulation of cell differentiation
- GO:0055007 cardiac muscle cell differentiation
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD29 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | Neat Pack Size: 0.1 mg, 25 µg 1/25 Pack Size: 0.5 mg |
1/10 Pack Size: 0.1 mg, 25 µg 1/100 Pack Size: 0.5 mg |
|
| Flow Cytometry | 1/50 | 1/100 | |
| Functional Assays | |||
| Immunoprecipitation | |||
| Flow Cytometry | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat | 1/5 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat F(ab')2 anti Hamster IgG:Dylight®800 | STAR104D800GA | F IF WB | 0.1 mg |
|
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| Goat F(ab')2 anti Hamster IgG:FITC | STAR104F | F | 0.4 mg |
|
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| Goat anti Hamster IgG:Biotin | STAR79B | E F | 0.8 mg |
|
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| Goat anti Hamster IgG:FITC | STAR79F | F | 0.7 mg |
|
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| Goat anti Hamster IgG:HRP | STAR79P | E | 0.8 mg |
|
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| Goat F(ab')2 anti Hamster IgG:Dylight®800 | STAR104D800GA | F IF WB | 0.1 mg |
|
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| Goat F(ab')2 anti Hamster IgG:FITC | STAR104F | F | 0.4 mg |
|
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| Goat anti Hamster IgG:Biotin | STAR79B | E F | 0.8 mg |
|
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| Goat anti Hamster IgG:FITC | STAR79F | F | 0.7 mg |
|
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| Goat anti Hamster IgG:HRP | STAR79P | E | 0.8 mg |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control:Alexa Fluor® 488 | MCA2356A488 | F | 100 Tests/1ml |
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| Hamster (Armenian) IgG Negative Control:Alexa Fluor® 647 | MCA2356A647 | F | 100 Tests/1ml |
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| Hamster (Armenian) IgG Negative Control:FITC | MCA2356F | F | 0.1 mg |
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| Hamster (Armenian) IgG Negative Control | MCA2356 | F | 0.25 mg |
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| Hamster (Armenian) IgG Negative Control | MCA2356 | F | 0.25 mg |
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| Hamster (Armenian) IgG Negative Control:RPE | MCA2356PE | F | 100 Tests/1ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
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| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
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| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
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| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
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| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
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| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
Positive Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control:Low Endotoxin | MCA2356EL | C F IF | 0.5 mg |
|
|
| Hamster (Armenian) IgG Negative Control:RPE | MCA2356PE | F | 100 Tests/1ml |
|
Product Specific References
References for CD29 antibody
-
Noto, K. et al. (1995) Identification and functional characterization of mouse CD29 with a mAb.
Int Immunol. 7 (5): 835-42. -
Lu, T.Y. et al. (2010) Epithelial cell adhesion molecule regulation is associated with the maintenance of the undifferentiated phenotype of human embryonic stem cells.
J Biol Chem. 285: 8719-32. -
Li S et al. (2010) Upregulation of CXCR4 favoring neural-like cells migration via AKT activation.
Neurosci Res. 67 (4): 293-9. -
Eto, D.S. et al. (2007) Integrin-mediated host cell invasion by type 1-piliated uropathogenic Escherichia coli.
PLoS Pathog. 3: e100. -
Tiede, B.J. et al. (2009) A novel mouse model for non-invasive single marker tracking of mammary stem cells in vivo reveals stem cell dynamics throughout pregnancy.
PLoS One. 4 (11): e8035. -
Kouros-Mehr H (2008) GATA-3 links tumor differentiation and dissemination in a luminal breast cancer model.
Cancer Cell. 13: 141-52. -
Xu, L. et al. (2017) Umbilical cord-derived mesenchymal stem cells on scaffolds facilitate collagen degradation via upregulation of MMP-9 in rat uterine scars.
Stem Cell Res Ther. 8 (1): 84. -
Hou, B. et al. (2018) Comparison of the Effects of BMSC-derived Schwann Cells and Autologous Schwann Cells on Remyelination Using a Rat Sciatic Nerve Defect Model.
Int J Biol Sci. 14 (13): 1910-1922. -
Porwal, K. et al. (2019) Increased bone marrow-specific adipogenesis by clofazimine causes impaired fracture healing, osteopenia and osteonecrosis without extra-skeletal effects in rats.
Toxicol Sci. kfz172. -
Zhu, C. et al. (2020) Antinociceptive effect of intrathecal injection of miR-9-5p modified mouse bone marrow mesenchymal stem cells on a mouse model of bone cancer pain.
J Neuroinflammation. 17 (1): 85.
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