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CD29 antibody | HM beta 1-1

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Anti Mouse CD29 Antibody, clone HM beta 1-1 gallery image 1
Figure A. RPE conjugated rat anti mouse CD3 (MCA500PE) and FITC conjugated hamster IgG isotype control (MCA2356F). Figure B. RPE conjugated rat anti mouse CD3 (MCA500PE) and FITC conjugated hamster anti mouse CD29 (MCA2298F). All experiments performed on red cell lysed murine peripheral blood gated on lymphocytes in the presence of murine SeroBlock (BUF041A).

Hamster anti Mouse CD29:Alexa Fluor® 488

Hamster anti Mouse CD29:Alexa Fluor® 647

Hamster anti Mouse CD29:FITC

Hamster anti Mouse CD29:Low Endotoxin

Hamster anti Mouse CD29

Hamster anti Mouse CD29:RPE

Product Type
Monoclonal Antibody
Clone
HM beta 1-1
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA2298A488T
Datasheet
F
MSDS
25 Tests/0.25ml loader

MCA2298A488
Datasheet
F
MSDS
100 Tests/1ml loader

MCA2298A647T
Datasheet
F
MSDS
25 Tests/0.25ml loader

MCA2298A647
Datasheet
F
MSDS
100 Tests/1ml loader

MCA2298FT
Datasheet
F
MSDS
25 µg loader

MCA2298F
Datasheet
F
MSDS
0.1 mg loader

MCA2298FB
Datasheet
F
MSDS
0.5 mg loader

MCA2298EL
Datasheet
F FN IP
MSDS
0.5 mg loader

MCA2298GA
Datasheet
F IP
MSDS
0.1 mg loader

MCA2298PE
Datasheet
F
MSDS
100 Tests loader

Hamster anti Mouse CD29 antibody, clone HM beta 1-1 recognizes the murine integrin beta 1 subunit (CD29), a ~110 kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. The ligands for these heterodimers include collagen, fibronectin, laminin and vascular adhesion molecule-1. In the immune system beta 1 integrins play an important role in cell adhesion, migration, activation and differentiation.

Hamster anti Mouse CD29 antibody, clone HM beta 1-1 is reported to inhibit beta 1 integrin mediated adhesion (Noto et al. 1995).

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - conjugated to Alexa Fluor®488 - liquid
Product Form
Purified IgG - conjugated to Alexa Fluor®647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Purified mouse VLA-4 antigen.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
Pack Size: 25 µg, 0.1 mg
IgG concentration 0.1 mg/ml
Pack Size: 0.5 mg
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Armenian hamsters were fused with cells of the P3U1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Storage
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.
Guarantee
12 months from date of reconstitution.

More Information

UniProt
P09055
Entrez Gene
Itgb1
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0001701 in utero embryonic development
GO:0001708 cell fate specification
GO:0051726 regulation of cell cycle
GO:0004872 receptor activity
GO:0005178 integrin binding
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0008284 positive regulation of cell proliferation
GO:0008354 germ cell migration
GO:0019717 synaptosome
GO:0031594 neuromuscular junction
GO:0042383 sarcolemma
GO:0042470 melanosome
GO:0045214 sarcomere organization
GO:0045596 negative regulation of cell differentiation
GO:0055007 cardiac muscle cell differentiation
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD29 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/5
Flow Cytometry Neat Pack Size: 25 µg, 0.1 mg
1/25 Pack Size: 0.5 mg		 1/10 Pack Size: 25 µg, 0.1 mg
1/100 Pack Size: 0.5 mg
Flow Cytometry 1/50 1/100
Functional Assays
Immunoprecipitation
Flow Cytometry
Immunoprecipitation
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA F IF WB 0.1 mg loader
Goat F(ab')2 anti Hamster IgG:FITC STAR104F F 0.4 mg loader
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA F IF WB 0.1 mg loader
Goat F(ab')2 anti Hamster IgG:FITC STAR104F F 0.4 mg loader
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Hamster (Armenian) IgG Negative Control:Alexa Fluor® 488 MCA2356A488 F 100 Tests/1ml loader
Hamster (Armenian) IgG Negative Control:Alexa Fluor® 647 MCA2356A647 F 100 Tests/1ml loader
Hamster (Armenian) IgG Negative Control:FITC MCA2356F F 0.1 mg loader
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader
Hamster (Armenian) IgG Negative Control:RPE MCA2356PE F 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader

Positive Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Hamster (Armenian) IgG Negative Control:Low Endotoxin MCA2356EL C F IF 0.5 mg loader
Hamster (Armenian) IgG Negative Control:RPE MCA2356PE F 0.1 mg loader

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for CD29 antibody

  1. Noto, K. et al. (1995) Identification and functional characterization of mouse CD29 with a mAb.
    Int Immunol. 7 (5): 835-42.
  2. Lu, T.Y. et al. (2010) Epithelial cell adhesion molecule regulation is associated with the maintenance of the undifferentiated phenotype of human embryonic stem cells.
    J Biol Chem. 285: 8719-32.
  3. Li S et al. (2010) Upregulation of CXCR4 favoring neural-like cells migration via AKT activation.
    Neurosci Res. 67 (4): 293-9.
  4. Eto, D.S. et al. (2007) Integrin-mediated host cell invasion by type 1-piliated uropathogenic Escherichia coli.
    PLoS Pathog. 3: e100.
  5. Tiede, B.J. et al. (2009) A novel mouse model for non-invasive single marker tracking of mammary stem cells in vivo reveals stem cell dynamics throughout pregnancy.
    PLoS One. 4 (11): e8035.
  6. Kouros-Mehr H (2008) GATA-3 links tumor differentiation and dissemination in a luminal breast cancer model.
    Cancer Cell. 13: 141-52.
  7. Xu, L. et al. (2017) Umbilical cord-derived mesenchymal stem cells on scaffolds facilitate collagen degradation via upregulation of MMP-9 in rat uterine scars.
    Stem Cell Res Ther. 8 (1): 84.
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