CD273 antibody | TY25
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Rat anti Mouse CD273
- Product Type
- Monoclonal Antibody
- Clone
- TY25
- Isotype
- IgG2a
- Specificity
- CD273
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD273 antibody, clone TY25 recognises mouse CD273, also known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). CD273 is a 247 amino acid, ~42 kDa member of the B7 family. The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4. CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production (Schmidt et al.2015). Rat anti Mouse CD273 antibody, clone TY25 is reported to block the binding of CD273 (B7-DC) to it's receptor (Matsumoto et al. 2004). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- B7-DC transfected RAW264.7 cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Lymph node cells from immunized SD rats were fused with the cells of the P3U1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | 1/200 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
Source Reference
-
Yamazaki, T. et al. (2002) Expression of programmed death 1 ligands by murine T cells and APC.
J Immunol. 169 (10): 5538-45.
References for CD273 antibody
-
Kanai, T. et al. (2003) Blockade of B7-H1 suppresses the development of chronic intestinal inflammation.
J Immunol. 171 (8): 4156-63. -
Matsumoto, K. et al. (2004) B7-DC regulates asthmatic response by an IFN-gamma-dependent mechanism.
J Immunol. 172 (4): 2530-41. -
Yamazaki, T. et al. (2005) Blockade of B7-H1 on macrophages suppresses CD4+ T cell proliferation by augmenting IFN-gamma-induced nitric oxide production.
J Immunol. 175: 1586-92. -
Furuhashi K et al. (2012) Mouse lung CD103+ and CD11b high dendritic cells preferentially induce distinct CD4+ T-cell responses.
Am J Respir Cell Mol Biol. 46 (2): 165-72. -
Lopez-Medina, M. et al. (2015) Salmonella induces PD-L1 expression in B cells.
Immunol Lett. 167 (2): 131-40. -
López-Medina, M. et al. (2015) Salmonella impairs CD8 T cell response through PD-1: PD-L axis.
Immunobiology. 220 (12): 1369-80.
- Synonyms
- PD-L2
- RRID
- AB_905919
- UniProt
- Q9WUL5
- Entrez Gene
- Pdcd1lg2
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0042102 positive regulation of T cell proliferation
- GO:0042130 negative regulation of T cell proliferation
MCA2465
150703
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