CD273 antibody | TY25
|Rat anti Mouse CD273 antibody, clone TY25 recognises mouse CD273, also known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). CD273 is a 247 amino acid, ~42 kDa member of the B7 family. The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4.
CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production (Schmidt et al.2015).
Rat anti Mouse CD273 antibody, clone TY25 is reported to block the binding of CD273 (B7-DC) to it's receptor (Matsumoto et al. 2004).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- B7-DC transfected RAW264.7 cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Lymph node cells from immunised SD rats were fused with the cells of the P3U1 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Yamazaki, T. et al. (2002) Expression of programmed death 1 ligands by murine T cells and APC.
J Immunol. 169 (10): 5538-45.
References for CD273 antibody
Matsumoto, K. et al. (2004) B7-DC regulates asthmatic response by an IFN-gamma-dependent mechanism.
J Immunol. 172 (4): 2530-41.
Kanai, T. et al. (2003) Blockade of B7-H1 suppresses the development of chronic intestinal inflammation.
J Immunol. 171 (8): 4156-63.
Yamazaki, T. et al. (2005) Blockade of B7-H1 on macrophages suppresses CD4+ T cell proliferation by augmenting IFN-gamma-induced nitric oxide production.
J Immunol. 175: 1586-92.
Furuhashi K et al. (2012) Mouse lung CD103+ and CD11b high dendritic cells preferentially induce distinct CD4+ T-cell responses.
Am J Respir Cell Mol Biol. 46 (2): 165-72.
López-Medina M et al. (2015) Salmonella induces PD-L1 expression in B cells.
Immunol Lett. pii: S0165-2478(15)30018-3.
López-Medina M et al. (2015) Salmonella impairs CD8 T cell response through PD-1: PD-L axis.
Immunobiology. pii: S0171-2985(15)30028-0.
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