CD273 antibody | TY25
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Rat anti Mouse CD273:Alexa Fluor® 488
Rat anti Mouse CD273:Alexa Fluor® 647
Rat anti Mouse CD273:FITC
Rat anti Mouse CD273:Pacific Blue®
Rat anti Mouse CD273
- Product Type
- Monoclonal Antibody
- Clone
- TY25
- Isotype
- IgG2a
Rat anti Mouse CD273 antibody, clone TY25 recognises mouse CD273, also known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). CD273 is a 247 amino acid, ~42 kDa member of the B7 family. The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4.
CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). Studies suggest that both CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production.
Rat anti Mouse CD273 antibody, clone TY25 is reported to block the binding of CD273 (B7-DC) to it's receptor (Matsumoto et al. 2004). Removal of sodium azide is recommended prior to use in functional assays, the use of EQU003 is recommended for this purpose.
CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). Studies suggest that both CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production.
Rat anti Mouse CD273 antibody, clone TY25 is reported to block the binding of CD273 (B7-DC) to it's receptor (Matsumoto et al. 2004). Removal of sodium azide is recommended prior to use in functional assays, the use of EQU003 is recommended for this purpose.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor®488- liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Pacific Blue® - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- B7-DC transfected RAW264.7 cells.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Lymph node cells from immunised SD rats were fused with the cells of the P3U1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- Q9WUL5 Related reagents
- Entrez Gene
- Pdcd1lg2 Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0042102 positive regulation of T cell proliferation
- GO:0042130 negative regulation of T cell proliferation
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD273 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/5 | 1/10 | |
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | 1/100 | 1/200 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B/C). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B/C). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B/C) - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B/C) - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | 1 ml | C E P WB | ||
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | 0.5 mg | C E IF P WB | ||
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | 0.1 mg | F IF WB | ||
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | 1 mg | F | ||
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | 1 mg | C E P RE | ||
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | 0.5 ml | F | ||
| Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) | STAR71D549GA | 0.1 mg | F IF | ||
| Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) | STAR71D649GA | 0.1 mg | F IF | ||
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | 0.1 mg | F IF WB | ||
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | 0.5 mg | C E P | ||
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | 0.5 ml | F |
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control:Alexa Fluor® 488 | MCA1212A488 | 100 Tests/1ml | F | ||
| Rat IgG2a Negative Control:Alexa Fluor® 647 | MCA1212A647 | 100 Tests/1ml | F | ||
| Rat IgG2a Negative Control:FITC | MCA1212F | 100 Tests | F | ||
| Rat IgG2a Negative Control:Pacific Blue® | MCA1212PB | 100 Tests/1ml | F | ||
| Rat IgG2a Negative Control | MCA1212 | 1 ml | E F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F |
Product Specific References
Source Reference
-
Yamazaki, T. et al. (2002) Expression of programmed death 1 ligands by murine T cells and APC.
J Immunol. 169 (10): 5538-45.
References for CD273 antibody
-
Matsumoto, K. et al. (2004) B7-DC regulates asthmatic response by an IFN-gamma-dependent mechanism.
J Immunol. 172 (4): 2530-41. -
Kanai, T. et al. (2003) Blockade of B7-H1 suppresses the development of chronic intestinal inflammation.
J Immunol. 171 (8): 4156-63. -
Yamazaki, T. et al. (2005) Blockade of B7-H1 on macrophages suppresses CD4+ T cell proliferation by augmenting IFN-gamma-induced nitric oxide production.
J Immunol. 175: 1586-92. -
Furuhashi K et al. (2012) Mouse lung CD103+ and CD11b high dendritic cells preferentially induce distinct CD4+ T-cell responses.
Am J Respir Cell Mol Biol. 46 (2): 165-72. -
López-Medina M et al. (2015) Salmonella induces PD-L1 expression in B cells.
Immunol Lett. pii: S0165-2478(15)30018-3. -
López-Medina M et al. (2015) Salmonella impairs CD8 T cell response through PD-1: PD-L axis.
Immunobiology. pii: S0171-2985(15)30028-0.
