CD27 antibody | LG.3A10

Hamster anti Mouse CD27

Product Type
Monoclonal Antibody
Clone
LG.3A10
Isotype
IgG
Specificity
CD27

Product Code Applications Pack Size List Price Your Price Qty
MCA4701
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SDS Safety Datasheet SDS
C F IP 0.25 mg loader
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Hamster anti Mouse CD27 antibody, clone LG.3A10 recognizes mouse, rat and human CD27, a member of the tumor necrosis factor receptor superfamily. It is expressed in the thymus and spleen, but not in non-lymphoid tissues. CD27 acts as a ligand for CD70, which is expressed in T and B cells, and acts as a co-stimulatory signal in lymphocyte proliferation and immunoglobulin production.

CD27 lacks a death domain in its short cytoplasmic tail, but can induce apoptosis by binding with the apoptosis regulatory protein Siva (also known as CD27-binding protein).

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Fusion Partners
Armenian hamster spleen cells from immunized animals were fused with cells of the murine SP2/0 non-secreting myeloma.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA F IF WB 0.1 mg loader
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Description Goat F(ab')2 anti Hamster IgG:Dylight®800
Goat F(ab')2 anti Hamster IgG:FITC STAR104F F 0.4 mg loader
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Description Goat F(ab')2 anti Hamster IgG:FITC
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
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Description Goat anti Hamster IgG:Biotin
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
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Description Goat anti Hamster IgG:FITC
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader
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Description Goat anti Hamster IgG:HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader
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Description Hamster (Armenian) IgG Negative Control

Source Reference

  1. Gravestein, L.A. et al. (1995) Novel mAbs reveal potent co-stimulatory activity of murine CD27.
    Int Immunol. 7: 551-7.

References for CD27 antibody

  1. Gravestein, L.A. et al. (1996) CD27 cooperates with the pre-T cell receptor in the regulation of murine T cell development.
    J Exp Med. 184 (2): 675-85.
  2. Sakanishi, T. & Yagita, H. (2010) Anti-tumor effects of depleting and non-depleting anti-CD27 monoclonal antibodies in immune-competent mice.
    Biochem Biophys Res Commun. 393 (4): 829-35.

RRID
AB_2073423
UniProt
P41272
P26842
Q501W2
Entrez Gene
CD27
Cd27
GO Terms
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0005576 extracellular region
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0006916 anti-apoptosis
GO:0006917 induction of apoptosis
GO:0007166 cell surface receptor linked signaling pathway
GO:0008588 release of cytoplasmic sequestered NF-kappaB
GO:0016064 immunoglobulin mediated immune response
GO:0043027 caspase inhibitor activity
GO:0045579 positive regulation of B cell differentiation
GO:0046330 positive regulation of JNK cascade
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0009986 cell surface
GO:0042100 B cell proliferation
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045582 positive regulation of T cell differentiation
GO:0048305 immunoglobulin secretion
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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