CD27 antibody | LG.3A10

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Hamster anti Mouse CD27:Biotin

Hamster anti Mouse CD27:FITC

Hamster anti Mouse CD27

Hamster anti Mouse CD27:RPE

Product Type
Monoclonal Antibody
Clone
LG.3A10
Isotype
IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4701B C F IP 0.1 mg
MCA4701F F 0.1 mg
MCA4701 C F IP 0.25 mg
MCA4701PE F 0.1 mg
Hamster anti Mouse CD27 antibody, clone LG.3A10 recogniszes mouse, rat and human CD27, a member of the tumour necrosis factor receptor superfamily. It is expressed in the thymus and spleen, but not in non-lymphoid tissues. CD27 acts as a ligand for CD70, which is expressed in T and B cells, and acts as a costimulatory signal in lymphocyte proliferation and immunoglobulin production.

CD27 lacks a death domain in its short cytoplasmic tail, but can induce apoptosis by binding with the apoptosis regulatory protein Siva (also known as CD27-binding protein).

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Approx. Protein Concentrations
IgG concentration 0.2mg/ml
Fusion Partners
Spleen cells from immunized Armenian Hamsters were fused with cells of the murine SP2/0 non-secreting myeloma.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P41272 Related reagents
P26842 Related reagents
Q501W2 Related reagents
Entrez Gene
CD27 Related reagents
Cd27 Related reagents
GO Terms
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0005576 extracellular region
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0006916 anti-apoptosis
GO:0006917 induction of apoptosis
GO:0007166 cell surface receptor linked signaling pathway
GO:0008588 release of cytoplasmic sequestered NF-kappaB
GO:0016064 immunoglobulin mediated immune response
GO:0043027 caspase inhibitor activity
GO:0045579 positive regulation of B cell differentiation
GO:0046330 positive regulation of JNK cascade
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0009986 cell surface
GO:0042100 B cell proliferation
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045582 positive regulation of T cell differentiation
GO:0048305 immunoglobulin secretion
Regulatory
For research purposes only

Applications of CD27 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20 1/50
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry 1/20 1/50
Flow Cytometry 1/10 1/20
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry 1/5 1/20
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat F(ab')2 anti Hamster IgG:DyLight®549 STAR104D549GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:DyLight®649 STAR104D649GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA 0.1 mg F IF WB
Goat F(ab')2 anti Hamster IgG:FITC STAR104F 0.4 mg F
Goat anti Hamster IgG:Biotin STAR79B 0.8 mg E F
Goat anti Hamster IgG:FITC STAR79F 0.7 mg F
Goat anti Hamster IgG:HRP STAR79P 0.8 mg E

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Hamster (Armenian) IgG Negative Control:FITC MCA2356F 0.1 mg F
Hamster (Armenian) IgG Negative Control MCA2356 0.25 mg F
Hamster (Armenian) IgG Negative Control:RPE MCA2356PE 0.1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Product Specific References

Source Reference

  1. Gravestein, L.A. et al. (1995) Novel mAbs reveal potent co-stimulatory activity of murine CD27.
    Int Immunol. 7: 551-7.

References for CD27 antibody

  1. Gravestein, L.A. et al. (1996) CD27 cooperates with the pre-T cell receptor in the regulation of murine T cell development.
    J Exp Med. 184 (2): 675-85.