CD25 antibody | PC61.5.3
Rat anti Mouse CD25:StarBright Violet 790
- Product Type
- Monoclonal Antibody
- Clone
- PC61.5.3
- Isotype
- IgG1
- Specificity
- CD25
Antibody Quality and Performance Guarantee
Figure B. FITC conjugated Rat anti Mouse CD4 (MCA4635F) and RPE conjugated rat anti mouse CD25 (MCA1260PE).
All experiments performed on murine splenocytes in the presence of murine SeroBlock (BUF041A).
Figure B. StarBright Violet 515 conjugated Rat anti Mouse CD19 (MCA1439SBV515) and StarBright UltraViolet 575 conjugated Rat anti Mouse CD25 (MCA1260SBUV575).
All experiments performed on mouse splenocytes treated with Concanavalin A for 24hrs gated on live single cells, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.
Filter by Application:
F Reset| Rat anti Mouse CD25 antibody, clone PC61.5.3 reacts with the low affinity alpha chain of the interleukin-2 receptor present on activated T and B cells in mice. Rat anti Mouse CD25 antibody, clone PC61.5.3 is reported to inhibit IL-2 binding and IL-2 dependent proliferation. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to StarBright Violet 790 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Immunogen
- B6.1 CTL cell line.
- Approx. Protein Concentrations
- For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- Fusion Partners
- Spleen cells from immunized OFA rats were fused with cells of the P3X63Ag8.653 mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 790 402 782 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
Neat |
- 1Staining at lower temperatures generally requires longer incubation to reach antibody binding equilibrium, and this effect is more pronounced for StarBright Dyes due to their larger size. For optimum staining, Bio-Rad recommends incubating samples either overnight at 4°C or 1 hour at room temperature.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse Seroblock FcR | ||||||
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse Seroblock FcR | ||||||
References for CD25 antibody
-
Ceredig, R. et al. (1985) Expression of interleukin-2 receptor as a differentiation marker on intrathymic stem cells.
Nature 314: 98-100. -
Lowenthal, J.W. et al. (1985) High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61.
J Immunol. 135 (6): 3988-94. -
Hashimoto, N. et al. (1986) Dissociation of interleukin 2-dependent and -independent B cell proliferation with monoclonal anti-interleukin 2 receptor antibody.
Eur J Immunol. 16 (3): 317-20. -
Moreau, J.L. et al. (1987) Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site.
Eur J Immunol. 17 (7): 929-35. -
Yaqoob, P. & Calder, P.C. (1997) Glutamine requirement of proliferating T lymphocytes.
Nutrition. 13 (7-8): 646-51. -
Scotland, R.S. et al. (2011) Sex differences in resident immune cell phenotype underlie more efficient acute inflammatory responses in female mice.
Blood. 118 (22): 5918-27. -
Karali, D. et al. (2016) T cell regulation by Phlomis lanata protein extracts in mice.
Pharm Biol. 54 (2): 207-14. -
Szuster-Ciesielska, A. et al. (2019) Immunogenic Evaluation of Ribosomal P-Protein Antigen P0, P1, and P2 and Pentameric Protein Complex P0-(P1-P2)2 of Plasmodium falciparum in a Mouse Model.
J Immunol Res. 2019: 9264217.
View The Latest Product References
-
Curina, G. et al. (2018) Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis. REV1 vaccine produced in bioreactor.
Vet Immunol Immunopathol. 198: 44-53. -
Arad, T. et al. (2021) CD200 -dependent and -independent immune-modulatory functions of neural stem cells.
Stem Cell Res. 56: 102559. -
Roca, C.P. et al. (2023) A cross entropy test allows quantitative statistical comparison of t-SNE and UMAP representations
Cell Reports Methods. 3 (1): 100390.
- Synonyms
- IL-2R Alpha Chain
- UniProt
- P01590
- Entrez Gene
- Il2ra
- GO Terms
- GO:0016021 integral to membrane
- GO:0004911 interleukin-2 receptor activity
- GO:0006924 activation-induced cell death of T cells
- GO:0009897 external side of plasma membrane
- GO:0042104 positive regulation of activated T cell proliferation
- GO:0042130 negative regulation of T cell proliferation
- GO:0043065 positive regulation of apoptosis
- GO:0046013 regulation of T cell homeostatic proliferation
- GO:0050728 negative regulation of inflammatory response
- View More GO Terms
MCA1260SBV790
100005498
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