CD223 antibody | C9B7W
Rat anti Mouse CD223:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
- Clone
- C9B7W
- Isotype
- IgG1
- Specificity
- CD223
Rat anti Mouse CD223 antibody, clone C9B7W recognizes murine lymphocyte activation gene-3 (LAG-3), a ~70 kDa activation-induced cell surface molecule that is also referred to as CD223. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion. Clone C9B7W recognizes an epitope within the D2 domain of CD223. Rat anti Mouse CD223 antibody, clone C9B7W is reported to block the in vitro function of murine LAG-3 but does not block binding of LAG-3 to MHC class II (Workman et al. 2002). |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Murine CD223 Ig fusion protein.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Cells from immunized Lewis rats were fused with cells of the Sp/20 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/5 |
Source Reference
-
Workman, C.J. et al. (2002) Phenotypic analysis of the murine CD4-related glycoprotein, CD223 (LAG-3).
Eur J Immunol. 32 (8): 2255-63.
References for CD223 antibody
-
Workman, C.J. et al. (2002) Cutting edge: molecular analysis of the negative regulatory function of lymphocyte activation gene-3.
J Immunol. 169 (10): 5392-5. -
Workman, C.J. & Vignali, D.A. (2005) Negative regulation of T cell homeostasis by lymphocyte activation gene-3 (CD223).
J Immunol. 174 (2): 688-95. -
Byrne, K.T. et al. (2011) Autoimmune melanocyte destruction is required for robust CD8+ memory T cell responses to mouse melanoma.
J Clin Invest. 121 (5): 1797-809. -
Ordway, D. et al. (2007) The hypervirulent Mycobacterium tuberculosis strain HN878 induces a potent TH1 response followed by rapid down-regulation.
J Immunol. 179: 522-31. -
Hu, Z. et al. (2013) Regulatory CD8+ T cells associated with erosion of immune surveillance in persistent virus infection suppress in vitro and have a reversible proliferative defect.
J Immunol. 191 (1): 312-22. -
Iwasaki, Y. et al. (2013) Egr-2 transcription factor is required for Blimp-1-mediated IL-10 production in IL-27-stimulated CD4+ T cells.
Eur J Immunol. 43: 1063-73. -
Woo, S.R. et al. (2010) Differential subcellular localization of the regulatory T-cell protein LAG-3 and the coreceptor CD4.
Eur J Immunol. 40: 1768-77.
- Synonyms
- LAG-3
- RRID
- AB_566649
- UniProt
- Q61790
- Entrez Gene
- Lag3
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0042289 MHC class II protein binding
- GO:0045085 negative regulation of interleukin-2 biosynthetic process
- GO:0045954 positive regulation of natural killer cell mediated cytotoxicity
- GO:0050868 negative regulation of T cell activation
MCA2386A488
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