CD197 antibody | 4B12

Rat anti Mouse CD197

Product Type
Monoclonal Antibody
Clone
4B12
Isotype
IgG2a
Specificity
CD197

Product Code Applications Pack Size List Price Your Price Qty
MCA2367GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP 0.1 mg loader
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loader
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Rat anti Mouse CD197 antibody, clone 4B12 recognizes murine C-C chemokine receptor type 7, also known as CD197, Epstein-Barr virus-induced G-protein coupled receptor 1, CCR7 or MIP-3 beta receptor. CD197 is a 354 amino acid ~43 kDa member of the G-protein coupled receptor family. In mice, CD197 is expressed on most naïve T cells, a subset of memory T cells, B cells, mature dendritic cells, NK cells, a subset of thymocytes and CD34+ macrophage progenitor cells.

CD197 is a functional receptor for chemokines CCL21 and CCL19. The receptor plays an important role in regulating the trafficking and homing of lymphocytes and dendritic cells to secondary lymphoid organs.

Rat anti Mouse CD197 antibody, clone 4B12 does not block the binding of the ligand to CD197.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
CCR7 rat basophilic leukaemia (RBL)-2H3 transfectants.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Lou/c rats were fused with cells of the mouse X63.Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/100
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

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References for CD197 antibody

  1. Ritter, U. et al. (2004) Analysis of the CCR7 expression on murine bone marrow-derived and spleen dendritic cells.
    J Leukoc Biol. 76 (2): 472-6.

Synonyms
CCR7
RRID
AB_566604
UniProt
P47774
Entrez Gene
Ccr7
GO Terms
GO:0016021 integral to membrane
GO:0006935 chemotaxis
GO:0006955 immune response
GO:0009897 external side of plasma membrane
GO:0016493 C-C chemokine receptor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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