Browsing Modes for Business Users

CD197 antibody | 4B12

Rat anti Mouse CD197:Alexa Fluor® 647

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price

Rat anti Mouse CD197 antibody, clone 4B12 recognizes murine C-C chemokine receptor type 7, also known as CD197, Epstein-Barr virus-induced G-protein coupled receptor 1, CCR7 or MIP-3 beta receptor. CD197 is a 354 amino acid ~43 kDa member of the G-protein coupled receptor family. In mice, CD197 is expressed on most naïve T cells, a subset of memory T cells, B cells, mature dendritic cells, NK cells, a subset of thymocytes and CD34+ macrophage progenitor cells.

CD197 is a functional receptor for chemokines CCL21 and CCL19. The receptor plays an important role in regulating the trafficking and homing of lymphocytes and dendritic cells to secondary lymphoid organs.

Rat anti Mouse CD197 antibody, clone 4B12 does not block the binding of the ligand to CD197.

Target Species
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
CCR7 rat basophilic leukaemia (RBL)-2H3 transfectants.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised Lou/c rats were fused with cells of the mouse X63.Ag8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
For research purposes only
12 months from date of despatch
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:Alexa Fluor® 647 MCA1212A647 F 100 Tests/1ml loader
List Price Your Price
Description Rat IgG2a Negative Control:Alexa Fluor® 647

References for CD197 antibody

  1. Ritter, U. et al. (2004) Analysis of the CCR7 expression on murine bone marrow-derived and spleen dendritic cells.
    J Leukoc Biol. 76 (2): 472-6.
  2. Parodi, B. et al. (2021) Hydroxycarboxylic Acid Receptor 2, a Pleiotropically Linked Receptor for the Multiple Sclerosis Drug, Monomethyl Fumarate. Possible Implications for the Inflammatory Response.
    Front Immunol. 12: 655212.

Entrez Gene
GO Terms
GO:0016021 integral to membrane
GO:0006935 chemotaxis
GO:0006955 immune response
GO:0009897 external side of plasma membrane
GO:0016493 C-C chemokine receptor activity


160555 165240

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with CD197 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products
Please note