CD197 antibody | 4B12
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F ResetRat anti Mouse CD197
- Product Type
- Monoclonal Antibody
- Clone
- 4B12
- Isotype
- IgG2a
- Specificity
- CD197
Antibody Quality and 
Unrivaled 
| Rat anti Mouse CD197 antibody, clone 4B12 recognizes murine C-C chemokine receptor type 7, also known as CD197, Epstein-Barr virus-induced G-protein coupled receptor 1, CCR7 or MIP-3 beta receptor. CD197 is a 354 amino acid ~43 kDa member of the G-protein coupled receptor family. In mice, CD197 is expressed on most naïve T cells, a subset of memory T cells, B cells, mature dendritic cells, NK cells, a subset of thymocytes and CD34+ macrophage progenitor cells. CD197 is a functional receptor for chemokines CCL21 and CCL19. The receptor plays an important role in regulating the trafficking and homing of lymphocytes and dendritic cells to secondary lymphoid organs. Rat anti Mouse CD197 antibody, clone 4B12 does not block the binding of the ligand to CD197. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- CCR7 rat basophilic leukaemia (RBL)-2H3 transfectants.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Lou/c rats were fused with cells of the mouse X63.Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/100 |
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
References for CD197 antibody
-
Ritter, U. et al. (2004) Analysis of the CCR7 expression on murine bone marrow-derived and spleen dendritic cells.
J Leukoc Biol. 76 (2): 472-6.
- Synonyms
- CCR7
- RRID
- AB_566604
- UniProt
- P47774
- Entrez Gene
- Ccr7
- GO Terms
- GO:0016021 integral to membrane
- GO:0006935 chemotaxis
- GO:0006955 immune response
- GO:0009897 external side of plasma membrane
- GO:0016493 C-C chemokine receptor activity
View more products with CD197 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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