CD154 antibody | 39H5

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Hamster anti Mouse CD154:FITC

Product Type: Monoclonal Antibody
Clone: 39H5
Isotype: IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
MCA1521F Datasheet	F SDS	0.1 mg	Log in		Get Quote

Hamster anti Mouse CD154 antibody, clone 39H5 recognizes the murine CD40 ligand, also known as CD40L or CD154. CD154 is a ~39 kDa cell surface glycoprotein . CD154 is expressed by activated CD4+ve T lymphocytes.

Hamster anti Mouse CD154 antibody, clone 39H5 is routinely tested in flow cytometry on mouse CD40L transfected L cells.

Product Details

Target Species

Mouse

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Immunogen

L cells transfected with CD40L.

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Guarantee: 12 months from date of despatch

More Information

UniProt: P27548
Entrez Gene: Cd40lg
GO Terms: GO:0005886 plasma membrane; GO:0016021 integral to membrane; GO:0007165 signal transduction; GO:0005125 cytokine activity; GO:0005164 tumor necrosis factor receptor binding; GO:0005174 CD40 receptor binding; GO:0005615 extracellular space; GO:0006916 anti-apoptosis; GO:0006954 inflammatory response; GO:0030168 platelet activation; GO:0030183 B cell differentiation; GO:0042100 B cell proliferation; GO:0045190 isotype switching; GO:0048305 immunoglobulin secretion; GO:0051023 regulation of immunoglobulin secretion
Regulatory: For research purposes only

Applications of CD154 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Hamster (Armenian) IgG Negative Control:FITC	MCA2356F	F	0.1 mg	Log in		Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Mouse Seroblock FcR	BUF041A	F	0.1 mg	Log in		Get Quote
Mouse Seroblock FcR	BUF041B	F	0.5 mg	Log in		Get Quote

Application Based External Images

Flow Cytometry

Product Specific References

References for CD154 antibody

Hogg, K.G. et al. (2003) IL-10 regulates early IL-12-mediated immune responses induced by the radiation-attenuated schistosome vaccine.
Int Immunol. 15 (12): 1451-9.
McGregor, C.M. et al. (2004) CD154 is a negative regulator of autoaggressive CD8+ T cells in type 1 diabetes.
Proc Natl Acad Sci U S A. 101 (25): 9345-50.
Dong, L. et al. (2003) An immunostimulatory oligodeoxynucleotide containing a cytidine-guanosine motif protects senescence-accelerated mice from lethal influenza virus by augmenting the T helper type 1 response
J Gen Virol. 84: 1623-8.
Neron. S, et al. (2005) Differential responses of human B-lymphocyte subpopulations to graded levels of CD40-CD154 interaction.
Immunology. 2005 Dec;116(4):454-63.
Roy, A. et al. (2001) Increased efficiency of gamma-irradiated versus mitomycin C-treated feeder cells for the expansion of normal human cells in long-term cultures
J Hematother Stem Cell Res. 2001 Dec;10(6): 873-80.
Mazar, J. et al. (2005) CD40 ligand (CD154) takes part in regulation of the transition to mononuclear cell dominance during peritonitis.
Kidney Int. 67: 1340-9.
Hacker, U.T. et al. (2006) Gene transfer preferentially selects MHC class I positive tumour cells and enhances tumour immunogenicity.
Cancer Immunol Immunother. 55 (5): 547-57.
Mazar, J. et al. (2005) CD40 ligand (CD154) takes part in regulation of the transition to mononuclear cell dominance during peritonitis.
Kidney Int. 67 (4): 1340-9.
Serba, S. et al. (2008) Transfection with CD40L induces tumour suppression by dendritic cell activation in an orthotopic mouse model of pancreatic adenocarcinoma.
Gut. 57 (3): 344-51.
Crother, T.R. et al. (2012) Plasmacytoid dendritic cells play a role for effective innate immune responses during Chlamydia pneumoniae infection in mice.
PLoS One. 7 (10): e48655.

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