CD154 antibody | 39H5

Hamster anti Mouse CD154 antibody, clone 39H5 is routinely tested in flow cytometry on mouse CD40L transfected L cells.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- L cells transfected with CD40L.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P27548
- Entrez Gene
- Cd40lg
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0007165 signal transduction
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005174 CD40 receptor binding
- GO:0005615 extracellular space
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- GO:0030168 platelet activation
- GO:0030183 B cell differentiation
- GO:0042100 B cell proliferation
- GO:0045190 isotype switching
- GO:0048305 immunoglobulin secretion
- GO:0051023 regulation of immunoglobulin secretion
- Regulatory
- For research purposes only
Applications of CD154 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Hamster (Armenian) IgG Negative Control:FITC | MCA2356F | F | 0.1 mg |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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|
Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Product Specific References
References for CD154 antibody
-
Hogg, K.G. et al. (2003) IL-10 regulates early IL-12-mediated immune responses induced by the radiation-attenuated schistosome vaccine.
Int Immunol. 15 (12): 1451-9. -
McGregor, C.M. et al. (2004) CD154 is a negative regulator of autoaggressive CD8+ T cells in type 1 diabetes.
Proc Natl Acad Sci U S A. 101 (25): 9345-50. -
Dong, L. et al. (2003) An immunostimulatory oligodeoxynucleotide containing a cytidine-guanosine motif protects senescence-accelerated mice from lethal influenza virus by augmenting the T helper type 1 response
J Gen Virol. 84: 1623-8. -
Neron. S, et al. (2005) Differential responses of human B-lymphocyte subpopulations to graded levels of CD40-CD154 interaction.
Immunology. 2005 Dec;116(4):454-63. -
Roy, A. et al. (2001) Increased efficiency of gamma-irradiated versus mitomycin C-treated feeder cells for the expansion of normal human cells in long-term cultures
J Hematother Stem Cell Res. 2001 Dec;10(6): 873-80. -
Mazar, J. et al. (2005) CD40 ligand (CD154) takes part in regulation of the transition to mononuclear cell dominance during peritonitis.
Kidney Int. 67: 1340-9. -
Hacker, U.T. et al. (2006) Gene transfer preferentially selects MHC class I positive tumour cells and enhances tumour immunogenicity.
Cancer Immunol Immunother. 55 (5): 547-57. -
Mazar, J. et al. (2005) CD40 ligand (CD154) takes part in regulation of the transition to mononuclear cell dominance during peritonitis.
Kidney Int. 67 (4): 1340-9. -
Serba, S. et al. (2008) Transfection with CD40L induces tumour suppression by dendritic cell activation in an orthotopic mouse model of pancreatic adenocarcinoma.
Gut. 57 (3): 344-51. -
Crother, T.R. et al. (2012) Plasmacytoid dendritic cells play a role for effective innate immune responses during Chlamydia pneumoniae infection in mice.
PLoS One. 7 (10): e48655.
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