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CD154 antibody | 39H5

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Hamster anti Mouse CD154:FITC

Product Type
Monoclonal Antibody
Clone
39H5
Isotype
IgG
Specificity
CD154

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Product Code Applications Pack Size List Price Your Price Qty
MCA1521F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Hamster anti Mouse CD154 antibody, clone 39H5 recognizes the murine CD40 ligand, also known as CD40L or CD154. CD154 is a ~39 kDa cell surface glycoprotein . CD154 is expressed by activated CD4+ve T lymphocytes.

Hamster anti Mouse CD154 antibody, clone 39H5 is routinely tested in flow cytometry on mouse CD40L transfected L cells.

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
L cells transfected with CD40L.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/BUF041B).

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control:FITC MCA2356F F 0.1 mg loader
>
List Price Your Price
loader
Description Hamster (Armenian) IgG Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

References for CD154 antibody

  1. Roy, A. et al. (2001) Increased efficiency of gamma-irradiated versus mitomycin C-treated feeder cells for the expansion of normal human cells in long-term cultures
    J Hematother Stem Cell Res. 2001 Dec;10(6): 873-80.
  2. Dong, L. et al. (2003) An immunostimulatory oligodeoxynucleotide containing a cytidine-guanosine motif protects senescence-accelerated mice from lethal influenza virus by augmenting the T helper type 1 response
    J Gen Virol. 84: 1623-8.
  3. Hogg, K.G. et al. (2003) IL-10 regulates early IL-12-mediated immune responses induced by the radiation-attenuated schistosome vaccine.
    Int Immunol. 15 (12): 1451-9.
  4. McGregor, C.M. et al. (2004) CD154 is a negative regulator of autoaggressive CD8+ T cells in type 1 diabetes.
    Proc Natl Acad Sci U S A. 101 (25): 9345-50.
  5. Mazar, J. et al. (2005) CD40 ligand (CD154) takes part in regulation of the transition to mononuclear cell dominance during peritonitis.
    Kidney Int. 67 (4): 1340-9.
  6. Neron. S, et al. (2005) Differential responses of human B-lymphocyte subpopulations to graded levels of CD40-CD154 interaction.
    Immunology. 2005 Dec;116(4):454-63.
  7. Hacker, U.T. et al. (2006) Gene transfer preferentially selects MHC class I positive tumour cells and enhances tumour immunogenicity.
    Cancer Immunol Immunother. 55 (5): 547-57.
  8. Serba, S. et al. (2008) Transfection with CD40L induces tumour suppression by dendritic cell activation in an orthotopic mouse model of pancreatic adenocarcinoma.
    Gut. 57 (3): 344-51.
    9. View The Latest Product References
  9. Crother, T.R. et al. (2012) Plasmacytoid dendritic cells play a role for effective innate immune responses during Chlamydia pneumoniae infection in mice.
    PLoS One. 7 (10): e48655.

Flow Cytometry

Synonyms
CD40 Ligand
RRID
AB_321591
UniProt
P27548
Entrez Gene
Cd40lg
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0007165 signal transduction
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005174 CD40 receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0030168 platelet activation
GO:0030183 B cell differentiation
GO:0042100 B cell proliferation
GO:0045190 isotype switching
GO:0048305 immunoglobulin secretion
GO:0051023 regulation of immunoglobulin secretion

MCA1521F

158679 1708

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