CD154 antibody | 39H5

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Hamster anti Mouse CD154:FITC

Product Type
Monoclonal Antibody
Clone
39H5
Isotype
IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1521F F 0.1 mg
Hamster anti Mouse CD154 antibody, clone 39H5 recognizes the murine CD40 ligand, also known as CD40L or CD154. CD154 is a ~39 kDa cell surface glycoprotein . CD154 is expressed by activated CD4+ve T lymphocytes.

Hamster anti Mouse CD154 antibody, clone 39H5 is routinely tested in flow cytometry on mouse CD40L transfected L cells.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
L cells transfected with CD40L.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P27548 Related reagents
Entrez Gene
Cd40lg Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0007165 signal transduction
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005174 CD40 receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0030168 platelet activation
GO:0030183 B cell differentiation
GO:0042100 B cell proliferation
GO:0045190 isotype switching
GO:0048305 immunoglobulin secretion
GO:0051023 regulation of immunoglobulin secretion
Regulatory
For research purposes only

Applications of CD154 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Hamster (Armenian) IgG Negative Control:FITC MCA2356F 0.1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD154 antibody

  1. Hogg, K.G. et al. (2003) IL-10 regulates early IL-12-mediated immune responses induced by the radiation-attenuated schistosome vaccine.
    Int Immunol. 15 (12): 1451-9.
  2. McGregor, C.M. et al. (2004) CD154 is a negative regulator of autoaggressive CD8+ T cells in type 1 diabetes.
    Proc Natl Acad Sci U S A. 101 (25): 9345-50.
  3. Dong, L. et al. (2003) An immunostimulatory oligodeoxynucleotide containing a cytidine-guanosine motif protects senescence-accelerated mice from lethal influenza virus by augmenting the T helper type 1 response
    J Gen Virol. 84: 1623-8.
  4. Neron. S, et al. (2005) Differential responses of human B-lymphocyte subpopulations to graded levels of CD40-CD154 interaction.
    Immunology. 2005 Dec;116(4):454-63.
  5. Roy, A. et al. (2001) Increased efficiency of gamma-irradiated versus mitomycin C-treated feeder cells for the expansion of normal human cells in long-term cultures
    J Hematother Stem Cell Res. 2001 Dec;10(6): 873-80.
  6. Mazar, J. et al. (2005) CD40 ligand (CD154) takes part in regulation of the transition to mononuclear cell dominance during peritonitis.
    Kidney Int. 67: 1340-9.
  7. Hacker, U.T. et al. (2006) Gene transfer preferentially selects MHC class I positive tumour cells and enhances tumour immunogenicity.
    Cancer Immunol Immunother. 55 (5): 547-57.
  8. Mazar, J. et al. (2005) CD40 ligand (CD154) takes part in regulation of the transition to mononuclear cell dominance during peritonitis.
    Kidney Int. 67 (4): 1340-9.
  9. Serba, S. et al. (2008) Transfection with CD40L induces tumour suppression by dendritic cell activation in an orthotopic mouse model of pancreatic adenocarcinoma.
    Gut. 57 (3): 344-51.
  10. Crother, T.R. et al. (2012) Plasmacytoid dendritic cells play a role for effective innate immune responses during Chlamydia pneumoniae infection in mice.
    PLoS One. 7 (10): e48655.