CD152 antibody | 1B8
Hamster anti Mouse CD152:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
- Clone
- 1B8
- Isotype
- IgG
- Specificity
- CD152
Hamster anti Mouse CD152 antibody, clone 1B8 recognizes mouse CD152, also known as CTLA-4 (cytotoxic T-lymphocyte protein 4), a structural homolog for CD28. CD152 binds the same ligands as CD28, namely CD80 (B7.1) and CD86 (B7.2), but with much higher affinity. However, while binding of CD28 enhances T cell function, binding of CD152 inhibits it. CD152 is not detected on naïve T-cells and is induced upon T-cell activation. In mice, lack of CD152 leads to massive lymphoproliferative disorder leading to tissue destruction and autoimmunity, suggesting a critical role for CD152 in limiting T-cell responses. CD152 has been shown to reduce IL-2 production after ligation and can inhibit T-cell expansion. This regulatory role and the high binding affinity of CD152 make it a potential therapy for autoimmune diseases such as rheumatoid arthritis. Hamster anti Mouse CD152 antibody, clone 1B8 may be used to enhance proliferation and pro-inflammatory cytokines of lymph node cells. It may also be used to enhance tumor immunity. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor 488 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Extracellular portion of mouse CD152 fused to a mouse IgG2a.
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Fusion Partners
- Lymph cells from immunized Armenian hamsters were fused with cells of the murine SP2/0 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Hamster (Armenian) IgG Negative Control:Alexa Fluor® 488 | MCA2356A488 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Hamster (Armenian) IgG Negative Control:Alexa Fluor® 488 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse Seroblock FcR | ||||||
Mouse Seroblock FcR | BUF041B | F | 0.5 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse Seroblock FcR |
References for CD152 antibody
-
Walunas, T. L. et al. (1994) CTLA-4 can function as a negative regulator of T cell activation.
Immunity 1: 405-413. -
Verhagen, J. et al. (2013) CTLA-4 controls the thymic development of both conventional and regulatory T cells through modulation of the TCR repertoire.
Proc Natl Acad Sci U S A. 110 (3): E221-30.
Further Reading
-
Karandikar, N.J. et al. (1996) CTLA-4: a negative regulator of autoimmune disease.
J Exp Med. 184 (2): 783-8.
- Synonyms
- CTLA-4
- RRID
- AB_10673813
- UniProt
- P09793
- Entrez Gene
- Ctla4
- GO Terms
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0009897 external side of plasma membrane
MCA4636A488
154385If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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