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IgA antibody | IgA5-3B

Mouse anti Monkey IgA:Biotin

Product Type
Monoclonal Antibody
Clone
IgA5-3B
Isotype
IgG1
Specificity
IgA

Product Code Applications Pack Size List Price Your Price Qty
MCA2553B
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Mouse anti Monkey IgA antibody, clone IgA5-3B recognizes monkey IgA and does not cross-react with IgG or IgM.

Clone IgA5-3B has been shown to detect secreted IgA in cat saliva and rhesus monkey jejunal washes.

Target Species
Monkey
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cat
Rhesus Monkey
Dog
Human
Marmoset
Spotted Hyena
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Purified IgA from serum.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used in a direct ELISA or as a detection antibody in a sandwich ELISA together with product code MCA1891 as a capture reagent.

References for IgA antibody

  1. Grant, C.K. (1995) Immunoglobulin changes associated with feline immunodeficiency virus infections. In: Feline immunology and immunodeficiency Eds. Willett, B.J. and Jarrett, O.
    Oxford University Press pp. 170-189.
  2. Grant, C.K. (1995) Purification and characterization of feline IgM and IgA isotypes and three subclasses of IgG. In: Feline immunology and immunodeficiency, Eds. Willett, B.J. and Jarrett, O.
    Oxford University Press pp. 95-107.
  3. Beatty, J.A. et al. (1998) Feline immunodeficiency virus (FIV)-associated lymphoma: a potential role for immune dysfunction in tumourigenesis.
    Vet Immunol Immunopathol. 65 (2-4): 309-22.
  4. Flies, A.S. et al. (2012) Development of a hyena immunology toolbox.
    Vet Immunol Immunopathol. 145 (1-2): 110-9.
  5. Holbrook, B.C. et al. (2015) Nonhuman primate infants have an impaired respiratory but not systemic IgG antibody response following influenza virus infection.
    Virology. 476: 124-33.

RRID
AB_808605

MCA2553B

149031 156197 157312 165610

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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