Tubulin antibody | AbD22584
Rhodamine conjugated Human anti tubulin antibody, clone AbD22584 (12004166) used as a loading control for western blotting on HeLa cell lysates.
Image caption:
β proteins impair autophagy.
(A) Western blot for LC3B-II in HeLa cell lysates under control conditions and upon treatment with 50 μM chloroquine. Tubulin was used as a loading control. (B) Quantification of LC3B-II levels. All conditions were normalized to mCherry (n = 6 independent experiments; one-way ANOVA with Tukey’s multiple-comparisons test).
From: Riera-Tur I, Schäfer T, Hornburg D, Mishra A, da Silva Padilha M, Fernández-Mosquera L, Feigenbutz D, Auer P, Mann M, Baumeister W, Klein R, Meissner F, Raimundo N, Fernández-Busnadiego R, Dudanova I.
Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicity.
Life Sci Alliance. 2021 Dec 21;5(3):e202101185.
doi: 10.26508/lsa.202101185.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies allow one-step detection of common housekeeping proteins like actin, tubulin, and GAPDH in your samples. These anti-actin, anti-tubulin, and anti-GAPDH antibodies are directly labeled with a rhodamine derivative (Exmax/Emmax = 530 nm/580 nm). The hFAB Rhodamine Antibodies were generated with Human Combinatorial Antibody Library (HuCAL®) Technology, providing exceptional specificity.
These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc™ MP Imaging System, and can be used in multiplex experiments with StarBright™ Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot.
Features and Benefits
Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity
Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting
Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species
Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins
These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc™ MP Imaging System, and can be used in multiplex experiments with StarBright™ Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot.
Features and Benefits
Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity
Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting
Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species
Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. and purified using NiNTA affinity chromatography. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain and conjugated to rhodamine - lyophilized
- Reconstitution
- Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at maximum speed on a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- >80% Sucrose
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% Bovine Serum Albumin
Storage Information
- Storage
- 1 year at -20oC lyophilized; 6 months at +4oC after resuspension.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P07437
- Entrez Gene
- TUBB
- GO Terms
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0003924 GTPase activity
- GO:0005829 cytosol
- GO:0005525 GTP binding
- GO:0005874 microtubule
- GO:0006928 cellular component movement
- GO:0007018 microtubule-based movement
- GO:0042267 natural killer cell mediated cytotoxicity
- GO:0042288 MHC class I protein binding
- GO:0051258 protein polymerization
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences. - Regulatory
- For research purposes only
Applications of Tubulin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1,000 | 1/10,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004163.pdf
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat ANTI-MOUSE IgG StarBright™ Blue 700 | 12004158 | WB | 400 µl |
|
|
| Goat ANTI-RABBIT IgG StarBright™ Blue 700 | 12004161 | WB | 400 µl |
|
|
| ANTI-ACTIN hFAB™ Rhodamine Antibody | 12004163 | WB | 200 µl |
|
|
| ANTI-ACTIN hFAB™ Rhodamine Antibody | 12004164 | WB | 40 µl |
|
|
| ANTI-GAPDH hFAB™ Rhodamine Antibody | 12004167 | WB | 200 µl |
|
|
| ANTI-GAPDH hFAB™ Rhodamine Antibody | 12004168 | WB | 40 µl |
|
|
| Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) | STAR117D800GA | F IF WB | 0.1 mg |
|
Product Specific References
References for Tubulin antibody
-
Riera-Tur, I. et al. (2022) Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicity.
Life Sci Alliance. 5(3):e202101185. -
Cotton, T.R. et al. (2021) Structural basis of K63-ubiquitin chain formation by the Gordon-Holmes syndrome RBR E3 ubiquitin ligase RNF216.
Mol Cell S1097-2765 (21) 01067-4.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
