Tubulin antibody | AbD22584

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ANTI-TUBULIN hFAB™ Rhodamine Antibody

Product Type
Monoclonal Antibody
HuCAL Fab monovalent
Product Code Applications Pack Size List Price Quantity
200 µl loader

40 µl loader

hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies allow one-step detection of common housekeeping proteins like actin, tubulin, and GAPDH in your samples. These anti-actin, anti-tubulin, and anti-GAPDH antibodies are directly labeled with a rhodamine derivative (Exmax/Emmax = 530 nm/580 nm). The hFAB Rhodamine Antibodies were generated with Human Combinatorial Antibody Library (HuCAL®) Technology, providing exceptional specificity.

These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc™ MP Imaging System, and can be used in multiplex experiments with StarBright™ Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot.

Features and Benefits

Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity
Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting
Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species
Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. and purified using NiNTA affinity chromatography. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain and conjugated to rhodamine - lyophilized
Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at maximum speed on a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
>80% Sucrose
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% Bovine Serum Albumin

Storage Information

1 year at -20oC lyophilized; 6 months at +4oC after resuspension.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
Guaranteed until date of expiry. Please see product label.

More Information

Entrez Gene
GO Terms
GO:0000086 G2/M transition of mitotic cell cycle
GO:0003924 GTPase activity
GO:0005829 cytosol
GO:0005525 GTP binding
GO:0005874 microtubule
GO:0006928 cellular component movement
GO:0007018 microtubule-based movement
GO:0042267 natural killer cell mediated cytotoxicity
GO:0042288 MHC class I protein binding
GO:0051258 protein polymerization
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
For research purposes only

Applications of Tubulin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1,000 1/10,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004163.pdf

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Goat ANTI-MOUSE IgG StarBright™ Blue 700 12004158 WB 400 µl loader
Goat ANTI-RABBIT IgG StarBright™ Blue 700 12004161 WB 400 µl loader
ANTI-ACTIN hFAB™ Rhodamine Antibody 12004163 WB 200 µl loader
ANTI-ACTIN hFAB™ Rhodamine Antibody 12004164 WB 40 µl loader
ANTI-GAPDH hFAB™ Rhodamine Antibody 12004167 WB 200 µl loader
ANTI-GAPDH hFAB™ Rhodamine Antibody 12004168 WB 40 µl loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader

Product Specific References

References for Tubulin antibody

  1. Riera-Tur, I. et al. (2022) Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicity.
    Life Sci Alliance. 5(3):e202101185.
  2. Cotton, T.R. et al. (2021) Structural basis of K63-ubiquitin chain formation by the Gordon-Holmes syndrome RBR E3 ubiquitin ligase RNF216.
    Mol Cell S1097-2765 (21) 01067-4.

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra