Tubulin antibody | AbD22584
Rhodamine conjugated Human anti tubulin antibody, clone AbD22584 (12004166) used as a loading control for western blotting on HeLa cell lysates.
Image caption:
β proteins impair autophagy.
(A) Western blot for LC3B-II in HeLa cell lysates under control conditions and upon treatment with 50 μM chloroquine. Tubulin was used as a loading control. (B) Quantification of LC3B-II levels. All conditions were normalized to mCherry (n = 6 independent experiments; one-way ANOVA with Tukey’s multiple-comparisons test).
From: Riera-Tur I, Schäfer T, Hornburg D, Mishra A, da Silva Padilha M, Fernández-Mosquera L, Feigenbutz D, Auer P, Mann M, Baumeister W, Klein R, Meissner F, Raimundo N, Fernández-Busnadiego R, Dudanova I.
Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicity.
Life Sci Alliance. 2021 Dec 21;5(3):e202101185.
doi: 10.26508/lsa.202101185.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Human FAB anti tubulin antibody, clone AbD22584 (12004166) used as a loading control for human cell line lysates in western blotting.
Image caption:
dCas9-based transcriptional activators display variable expression and transactivation potencies based upon fused effector system, cell type and targeted locus. (B) Expression levels of indicated dCas9-based transcriptional activators 72 h after transient transfection into HEK293T (top), HeLa (middle), or K562 (bottom) cells.
From:Wang K, Escobar M, Li J, Mahata B, Goell J, Shah S, Cluck M, Hilton IB. Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions.
Nucleic Acids Res. 2022 Aug 12;50(14):7842-55.
doi: 10.1093/nar/gkac582.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rhodamine conjugated anti tubulin antibody, clone AbD22584 (1204166) used as a loading control for western blotting analysis of PDZ proteins.
Image caption:
IPDZ proteins interact with GFP-SARS-CoV-2 E protein via PDZ/PBM. GFP, GFP-SARS-CoV-2 E protein (E wt) or a version lacking the PBM (E stop) were transfected into the indicated cell line (HEK-293T or THP-1), together with a construct encoding a tagged version of the PDZ proteins (A) syntenin (HA-syntenin; (B) ZO-2 (Myc-ZO-2); or (C) IL-16 (Myc-IL-16.) Cells were lysed (see Section 4) and the whole cell lysate (WCL) was analyzed for expression of the GFP-E versions and for that of the PDZ proteins using the indicated Ab (GFP, HA, ZO-2, or IL-16) by Western blot. Tubulin was used as loading control (left). GFP and GFP-E proteins were immunoprecipitated using the GFP-trap system, and immunoprecipitates analyzed by Western blot (right). In the case of Ab against GFP, syntenin, ZO-2 and IL-16 were used.
From: Ávila-Flores A, Sánchez-Cabezón JJ, Ochoa-Echeverría A, Checa AI, Rosas-García J, Téllez-Araiza M, Casado S, Liébana R, Santos-Mendoza T, Mérida I.
Identification of Host PDZ-Based Interactions with the SARS-CoV-2 E Protein in Human Monocytes.
Int J Mol Sci. 2023 Aug 14;24(16):12793.
doi: 10.3390/ijms241612793..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti-tubulin hFAB™ Rhodamine antibody (12004167) used as a loading control in western blotting of skin cell lysates.
Image caption:
ALLN inhibits the fibrogenic activity of cultured human dermal fibroblasts stimulated with TGF-β1. Normal human dermal fibroblasts were pretreated with DMSO as control or various concentrations of DMSO-diluted ALLN for 1 h, followed by stimulation with 10 ng/ml recombinant human TGF-β1 for an additional 24 h. After harvesting, protein expression of the indicated molecules were evaluated by western blotting (B). Values were normalized to tubulin levels and are shown as relative levels (mean ± SEM). All values represent mean ± SEM; n = 5 each group; *, p ≤ 0.05; **, p ≤ 0.01, ***, p ≤ 0.001.
From: Kasamatsu H, Chino T, Hasegawa T, Utsunomiya N, Utsunomiya A, Yamada M, Oyama N, Hasegawa M.
A cysteine proteinase inhibitor ALLN alleviates bleomycin-induced skin and lung fibrosis.
Arthritis Res Ther. 2023 Aug 25;25(1):156.
doi: 10.1186/s13075-023-03130-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rhodamine conjugated Human anti tubulin antibody (12004165) used as a western blotting loading control for the analysis of phosphorylation in human NSCLC cell line lysates.
Image caption:
The bivalent inhibitor 4 suppresses EGFR phosphorylation across diverse human NSCLC cell lines.
Impact of 4 on EGFR phosphorylation (pY1068) in a H1975, b H3255, and c HCC827 cell line models. a Dosing of 4 in H1975 cells also diminishes phosphorylation of ERK1/2 and ATK downstream signaling kinases. Western blot experiments performed after 6-hour treatments and representative of at least three independent experiments (N = 3).
From: Wittlinger F, et al.
Linking ATP and allosteric sites to achieve superadditive binding with bivalent EGFR kinase inhibitors.
Commun Chem. 2024 Feb 20;7(1):38.
doi: 10.1038/s42004-024-01108-3.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rhodamine conjugated anti tubulin hFAB™ antibody (12004165) used as a loading control for evaluation of NRF2 signaling in HaCaT cell lysates by western blotting.
Image caption:
Calceolarioside E (CAL) activates NRF2 signaling in UVA/UVB-photoaged HaCaT cells. Western blot of STAT1 (A) and NRF2 (B) 24 h after UVA/UVB irradiation and 1 hr pre-treatment with HRE (1, 5, and 10 μg/mL), MYC, or CAL (1, 5, and 10 μM) and representative bands from the Western blot analysis (C). The results are presented as the mean ± SEM from three independent experiments. ** p < 0.01 compared to the UVA/UVB group.
From: Stoykova ID, Koycheva IK, Binev BK, Mihaylova LV, Benina MY, Alipieva KI, Georgiev MI.
Myconoside and Calceolarioside E Restrain UV-Induced Skin Photoaging by Activating NRF2-Mediated Defense Mechanisms.
Int J Mol Sci. 2024 Feb 19;25(4):2441.
doi: 10.3390/ijms25042441.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rhodamine conjugated Human anti Human Tubulin antibody (12004165) used to tubulin as a loading control in western blotting.
Image caption:
Blocking SVIP’s interaction with VCP rescues toxicity.
SVIP-mCherry with multiple site mutations in the VCP-interacting motif (VIMm-SVIP-mCherry) was generated and co-expressed with R155H-VCP-GFP in HEK293T cells. A Co-immunoprecipitation results depict that the VIMm-SVIP-mCherry does not interact with WT-VCP, but does with R155H-VCP and rescues R155H-VCP protein expression.
From:Ramzan F, Kumar A, Abrar F, Gray RAV, Campbell ZE, Liao LMQ, Dang A, Akanni O, Guyn C, Martin DDO.
Fatty links between multisystem proteinopathy and small VCP-interacting protein.
Cell Death Discov. 2024 Aug 8;10(1):358.
doi: 10.1038/s41420-024-02118-9.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
ANTI-TUBULIN hFAB™ Rhodamine Antibody
- Product Type
- Monoclonal Antibody
- Clone
- AbD22584
- Isotype
- HuCAL Fab monovalent
- Specificity
- Tubulin
Antibody Quality and Performance Guarantee
| hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies allow one-step detection of common housekeeping proteins like actin, tubulin, and GAPDH in your samples. These anti-actin, anti-tubulin, and anti-GAPDH antibodies are directly labeled with a rhodamine derivative (Exmax/Emmax = 530 nm/580 nm). The hFAB Rhodamine Antibodies were generated with Human Combinatorial Antibody Library (HuCAL®) Technology, providing exceptional specificity. These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc™ MP Imaging System, and can be used in multiplex experiments with StarBright™ Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot. Features and Benefits Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A monovalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. and purified using NiNTA affinity chromatography. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain and conjugated to rhodamine - lyophilized
- Reconstitution
- Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at maximum speed on a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- >80% Sucrose
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% bovine serum albumin - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Rhodamine 530 580 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
1 year at -20°C lyophilized; 6 months at +4°C after resuspension.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1,000 | 1/10,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
-
12004166
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004163.pdf. -
12004165
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004163.pdf
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for Tubulin antibody
-
Riera-Tur, I. et al. (2022) Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicity.
Life Sci Alliance. 5(3):e202101185. -
Cotton, T.R. et al. (2021) Structural basis of K63-ubiquitin chain formation by the Gordon-Holmes syndrome RBR E3 ubiquitin ligase RNF216.
Mol Cell S1097-2765 (21) 01067-4. -
Cejas, R.B. et al. (2022) Impact of DYRK1A Expression on TNNT2 Splicing and Daunorubicin Toxicity in Human iPSC-Derived Cardiomyocytes.
Cardiovasc Toxicol. May 21 [Epub ahead of print]. -
Wang, K. et al. (2022) Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions.
Nucleic Acids Res. 50 (14): 7842-55. -
Voelkl, K. et al. (2023) Neuroprotective effects of hepatoma-derived growth factor in models of Huntington’s disease
BioRχiv. 24 Feb Preprint. -
Ávila-Flores, A. et al. (2023) Identification of Host PDZ-Based Interactions with the SARS-CoV-2 E Protein in Human Monocytes.
Int J Mol Sci. 24 (16): 12793. -
Kasamatsu, H. et al. (2023) A cysteine proteinase inhibitor ALLN alleviates bleomycin-induced skin and lung fibrosis.
Arthritis Res Ther. 25 (1): 156. -
Hsia, O. et al. (2024) Targeted protein degradation via intramolecular bivalent glues.
Nature. 627 (8002): 204-11.
View The Latest Product References
-
Wittlinger, F. et al. (2024) Linking ATP and allosteric sites to achieve superadditive binding with bivalent EGFR kinase inhibitors.
Commun Chem. 7 (1): 38. -
Stoykova, I.D. et al. (2024) Myconoside and Calceolarioside E Restrain UV-Induced Skin Photoaging by Activating NRF2-Mediated Defense Mechanisms.
Int J Mol Sci. 25 (4) :2441. -
Tomoshige, S. et al. (2024) A small-molecule degron with a phenylpropionic acid scaffold.
Bioorg Med Chem. 109: 117789. -
Yamada, A. et al. (2024) Cellular memory function from 3D to 2D: Three-dimensional high density collagen microfiber cultures induce their resistance to reactive oxygen species
Mater Today Bio 26: 101097. -
Gautam, R. et al. (2024) Proton pump inhibitors modulate esophageal epithelial barrier function and crosstalk with eosinophils.
bioRχiv. Aug 23 [Epub ahead of print]. -
Ramzan, F. et al. (2024) Fatty links between multisystem proteinopathy and small VCP-interacting protein.
Cell Death Discov. 10 (1): 358.
- UniProt
- P07437
- Entrez Gene
- TUBB
- GO Terms
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0003924 GTPase activity
- GO:0005829 cytosol
- GO:0005525 GTP binding
- GO:0005874 microtubule
- GO:0006928 cellular component movement
- GO:0007018 microtubule-based movement
- GO:0042267 natural killer cell mediated cytotoxicity
- GO:0042288 MHC class I protein binding
- View More GO Terms
- GO:0051258 protein polymerization
View more products with TUBULIN specificity
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