Mouse anti Human tPA antibody, clone D12-9A7,
recognizes tissue type plasminogen activator (tPA) also known as PLAT. tPA is a serine protease that in the intravascular space, catalyzes the conversion of plasminogen to plasmin. tPA is expressed in various cell types including endothelial cells, glial cells and neurons. Within the brain tPA plays a role in synaptic plasticity, detection and adaptation to metabolic stress, modulation of the blood-brain barrier permeability and remodeling of the extracellular matrix (Jeanneret & Yepes 2017
The biotinylated Mouse anti Human tPA antibody, clone D12-9A7 (MCA6283B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human tPA antibody, clone D11-7G8 (MCA6282GA
) as the capture antibody.
- Target Species
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- Purified native human tPA
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Tpa antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
|Mouse anti Human Tpa
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra