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Synaptophysin antibody | SP15

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Anti Human Synaptophysin Antibody, clone SP15 thumbnail image 1 Anti Human Synaptophysin Antibody, clone SP15 thumbnail image 2
Anti Human Synaptophysin Antibody, clone SP15 gallery image 1
Immunohistochemical staining of formalin fixed, paraffin embedded human pancreas with Mouse anti Human synaptophysin antibody, clone SP15 (MCA1307).
Anti Human Synaptophysin Antibody, clone SP15 gallery image 2
Published customer image:
Mouse anti Human Synaptophysin antibody, clone SP15 (MCA1307) used to identify synaptic vesicles in pig retina by immunofluorescence.
Image caption:
 Bar graph showing the effect of dose on pRPC engraftment (A). Photomicrograph of 5-μm section of retina taken at 12 weeks after treatment showing processes of donor cells (arrowheads) stratified in a specific band in the outer plexiform layer (OPL) (*). Green: pRPC; Red: synaptophysin. (B). Recoverin (REC) GFP DAPI histology at 12 weeks of survival. It is worth noting that the incidence of GFP/ REC-positive cells was higher in integrated cells as compared to those remaining in the subretinal space (C–J).

From: Abud MB, Baranov P, Patel S, Hicks CA, Isaac DLC, Louzada RN, Dromel P, Singh D, Sinden J, Ávila MP, Young M.
In vivo study to assess dosage of allogeneic pig retinal progenitor cells: Long-term survival, engraftment, differentiation and safety.
J Cell Mol Med. 2022 Apr 28 [Epub ahead of print].
doi: 10.1111/jcmm.17332. .
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti Human Synaptophysin

Product Type
Monoclonal Antibody
Clone
SP15
Isotype
IgM
Specificity
Synaptophysin

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Product Code Applications Pack Size List Price Your Price Qty
MCA1307
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 0.2 mg loader
List Price Your Price
loader

Mouse anti Human Synaptophysin antibody, clone SP15 recognizes human synaptophysin, also known as Major synaptic vesicle protein p38. Synaptophysin is a 313 amino acid synaptic vesicle associated protein of ~38kDa containing a single marvel domain. Synaptophysin expression is ubiquitous in synaptic vesicle membranes where it is the most abundant protein and is involved in the regulation of endocytosis (Kwon et al. 2011)

Loss and atypical expression of synaptophysin is noted in conditions such as Huntindon's disease (Goto and Hirano 1990) and Alzheimer's disease (Masliah et al. 1989). Mutations in the synaptophysin gene have been identified and lead to a condition, Mental retardation, X-linked 96 (Tarpey et al. 2009).

Mouse anti human synaptophysin antibody, clone SP15 has been used for the detection of synaptophysin by immunofluorescence, immunohistochemistry and western blotting on murine (Vitry et al. 2009), marmoset (Ramirez et al. 2001), rat (Barr et al. 2004), lapine (Roher et al. 2000) and human tissues (Beach et al. 1997).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Hamster
Rat
Marmoset
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Immunogen
Crude human synaptic immunoprecipitate.
Approx. Protein Concentrations
Isotype concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NSO mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1 1/25
Western Blotting 1/500
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Pancreas

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References for Synaptophysin antibody

  1. Honer, W.G. et al. (1994) Hippocampal synaptic pathology in patients with temporal lobe epilepsy.
    Acta Neuropathol. 87 (2): 202-10.
  2. Honer, W.G. et al. (1992) Regional synaptic pathology in Alzheimer's disease.
    Neurobiol Aging. 13 (3): 375-82.
  3. Honer, W.G. et al. (1993) Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex.
    Brain Res. 609: 9-20.
  4. Honer, W.G. et al. (1992) Characterization of a synaptic antigen of interest in neuropsychiatric illness.
    Biol Psychiatry. 31 (2): 147-58.
  5. Honer, W.G. et al. (1989) Monoclonal antibodies to study the brain in schizophrenia.
    Brain Res. 500 (1-2): 379-83.
  6. Masliah, E. et al. (1994) Topographical distribution of synaptic-associated proteins in the neuritic plaques of Alzheimer's disease hippocampus.
    Acta Neuropathol. 87 (2): 135-42.
  7. Del Bigio, M.R. et al. (2003) Chronic hydrocephalus in rats and humans: white matter loss and behavior changes.
    Ann Neurol. 53 (3): 337-46.
  8. Wakabayashi, K. et al. (1994) Synapse alterations in the hippocampalentorhinal formation in Alzheimer's disease with and without Lewy body disease.
    Brain Research 667: 24-32.
    9. View The Latest Product References
  9. Dickson, D.W. et al. (1994) Hippocampal sclerosis: a common pathological feature of dementia in very old (> or = 80 years of age) humans.
    Acta Neuropathol. 88 (3): 212-21.
  10. Dickson, D.W. et al. (1995) Correlations of synaptic and pathological markers with cognition of the elderly.
    Neurobiol Aging 16: 285-98.
  11. Vitry, S. et al. (2010) Enhanced degradation of synaptophysin by the proteasome in mucopolysaccharidosis type IIIB.
    Mol Cell Neurosci. 41: 8-18.
  12. Khan, O.H. et al. (2006) Brain damage in neonatal rats following kaolin induction of hydrocephalus.
    Exp Neurol. 200 (2): 311-20.
  13. Abud, M.B. et al. (2022) In vivo. study to assess dosage of allogeneic pig retinal progenitor cells: Long-term survival, engraftment, differentiation and safety.
    J Cell Mol Med. 26(11):3254-68.

RRID
AB_2198861
UniProt
P08247
Entrez Gene
SYP
GO Terms
GO:0005509 calcium ion binding
GO:0005215 transporter activity
GO:0015485 cholesterol binding
GO:0016188 synaptic vesicle maturation
GO:0019717 synaptosome
GO:0030054 cell junction
GO:0030285 integral to synaptic vesicle membrane
GO:0048169 regulation of long-term neuronal synaptic plasticity
GO:0048172 regulation of short-term neuronal synaptic plasticity
GO:0048499 synaptic vesicle membrane organization

MCA1307

148299

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