Synaptophysin antibody | SP15

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Mouse anti Human Synaptophysin

Product Type
Monoclonal Antibody
Clone
SP15
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1307T P* 20 µg
MCA1307 E P* WB 0.2 mg
Mouse anti Human Synaptophysin antibody, clone SP15 recognizes human synaptophysin, also known as Major synaptic vesicle protein p38. Synaptophysin is a 313 amino acid synaptic vesicle associated protein of ~38kDa containing a single marvel domain. Synaptophysin expression is ubiquitous in synaptic vesicle membranes where it is the most abundant protein and is involved in the regulation of endocytosis (Kwon et al. 2011)

Loss and atypical expression of synaptophysin is noted in conditions such as Huntindon's disease (Goto and Hirano 1990) and Alzheimer's disease (Masliah et al. 1989). Mutations in the synaptophysin gene have been identified and lead to a condition, Mental retardation, X-linked 96 (Tarpey et al. 2009).

Mouse anti human synaptophysin antibody, clone SP15 has been used for the detection of synaptophysin by immunofluorescence, immunohistochemistry and western blotting on murine (Vitry et al. 2009), marmoset (Ramirez et al. 2001), rat (Barr et al. 2004), lapine (Roher et al. 2000) and human tissues (Beach et al. 1997).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Hamster
Rat
Marmoset
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by gel filtration from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Crude human synaptic immunoprecipitate.
Approx. Protein Concentrations
Isotype concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NSO mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P08247 Related reagents
Entrez Gene
SYP Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0005215 transporter activity
GO:0015485 cholesterol binding
GO:0016188 synaptic vesicle maturation
GO:0019717 synaptosome
GO:0030054 cell junction
GO:0030285 integral to synaptic vesicle membrane
GO:0048169 regulation of long-term neuronal synaptic plasticity
GO:0048172 regulation of short-term neuronal synaptic plasticity
GO:0048499 synaptic vesicle membrane organization
Regulatory
For research purposes only

Applications of Synaptophysin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1 1/25
Western Blotting 1/500
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Pancreas

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Product Specific References

References for Synaptophysin antibody

  1. Honer, W.G. et al. (1994) Hippocampal synaptic pathology in patients with temporal lobe epilepsy.
    Acta Neuropathol. 87 (2): 202-10.
  2. Honer, W.G. et al. (1992) Regional synaptic pathology in Alzheimer's disease.
    Neurobiol Aging. 13 (3): 375-82.
  3. Honer, W.G. et al. (1993) Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex.
    Brain Res. 609: 9-20.
  4. Honer, W.G. et al. (1992) Characterization of a synaptic antigen of interest in neuropsychiatric illness.
    Biol Psychiatry. 31 (2): 147-58.
  5. Honer, W.G. et al. (1989) Monoclonal antibodies to study the brain in schizophrenia.
    Brain Res. 500 (1-2): 379-83.
  6. Masliah, E. et al. (1994) Topographical distribution of synaptic-associated proteins in the neuritic plaques of Alzheimer's disease hippocampus.
    Acta Neuropathol. 87 (2): 135-42.
  7. Simpson, I.A. et al. (1994) Decreased concentrations of GLUT1 and GLUT3 glucose transporters in the brains of patients with Alzheimer's disease.
    Ann Neurol. 35 (5): 546-51.
  8. Wakabayashi, K. et al. (1994) Synapse alterations in the hippocampalentorhinal formation in Alzheimer's disease with and without Lewy body disease.
    Brain Research 667: 24-32.
  9. Dickson, D.W. et al. (1994) Hippocampal sclerosis: a common pathological feature of dementia in very old (> or = 80 years of age) humans.
    Acta Neuropathol. 88 (3): 212-21.
  10. Dickson, D.W. et al. (1995) Correlations of synaptic and pathological markers with cognition of the elderly.
    Neurobiol Aging 16: 285-98.
  11. Vitry, S. et al. (2010) Enhanced degradation of synaptophysin by the proteasome in mucopolysaccharidosis type IIIB.
    Mol Cell Neurosci. 41: 8-18.
  12. Khan, O.H. et al. (2006) Brain damage in neonatal rats following kaolin induction of hydrocephalus.
    Exp Neurol. 200 (2): 311-20.
  13. Del Bigio, M.R. et al. (2003) Chronic hydrocephalus in rats and humans: white matter loss and behavior changes.
    Ann Neurol. 53 (3): 337-46.
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