Synaptophysin antibody | SP15
Loss and atypical expression of synaptophysin is noted in conditions such as Huntindon's disease (Goto and Hirano 1990) and Alzheimer's disease (Masliah et al. 1989). Mutations in the synaptophysin gene have been identified and lead to a condition, Mental retardation, X-linked 96 (Tarpey et al. 2009).
Mouse anti human synaptophysin antibody, clone SP15 has been used for the detection of synaptophysin by immunofluorescence, immunohistochemistry and western blotting on murine (Vitry et al. 2009), marmoset (Ramirez et al. 2001), rat (Barr et al. 2004), lapine (Roher et al. 2000) and human tissues (Beach et al. 1997).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Hamster Rat Marmoset
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgM - liquid
- Purified IgM prepared by ammonium sulphate precipitation
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Crude human synaptic immunoprecipitate.
- Approx. Protein Concentrations
- Isotype concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NSO mouse myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- P08247 Related reagents
- Entrez Gene
- SYP Related reagents
- GO Terms
- GO:0005509 calcium ion binding
- GO:0005215 transporter activity
- GO:0015485 cholesterol binding
- GO:0016188 synaptic vesicle maturation
- GO:0019717 synaptosome
- GO:0030054 cell junction
- GO:0030285 integral to synaptic vesicle membrane
- GO:0048169 regulation of long-term neuronal synaptic plasticity
- GO:0048172 regulation of short-term neuronal synaptic plasticity
- GO:0048499 synaptic vesicle membrane organization
- For research purposes only
Applications of Synaptophysin antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1||1/25|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Human anti Mouse IgM:FITC||HCA040F||F||0.1 mg|
|Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)||STAR138A||C E P WB||1 ml|
|Goat anti Mouse IgG/A/M:Alk. Phos.||STAR87A||C E WB||1 mg|
|Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)||STAR87P||E||1 mg|
Product Specific References
References for Synaptophysin antibody
Honer, W.G. et al. (1994) Hippocampal synaptic pathology in patients with temporal lobe epilepsy.
Acta Neuropathol. 87 (2): 202-10.
Honer, W.G. et al. (1992) Regional synaptic pathology in Alzheimer's disease.
Neurobiol Aging. 13 (3): 375-82.
Honer, W.G. et al. (1993) Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex.
Brain Res. 609: 9-20.
Honer, W.G. et al. (1992) Characterization of a synaptic antigen of interest in neuropsychiatric illness.
Biol Psychiatry. 31 (2): 147-58.
Honer, W.G. et al. (1989) Monoclonal antibodies to study the brain in schizophrenia.
Brain Res. 500 (1-2): 379-83.
Masliah, E. et al. (1994) Topographical distribution of synaptic-associated proteins in the neuritic plaques of Alzheimer's disease hippocampus.
Acta Neuropathol. 87 (2): 135-42.
Simpson, I.A. et al. (1994) Decreased concentrations of GLUT1 and GLUT3 glucose transporters in the brains of patients with Alzheimer's disease.
Ann Neurol. 35 (5): 546-51.
Wakabayashi, K. et al. (1994) Synapse alterations in the hippocampalentorhinal formation in Alzheimer's disease with and without Lewy body disease.
Brain Research 667: 24-32.
Dickson, D.W. et al. (1994) Hippocampal sclerosis: a common pathological feature of dementia in very old (> or = 80 years of age) humans.
Acta Neuropathol. 88 (3): 212-21.
Dickson, D.W. et al. (1995) Correlations of synaptic and pathological markers with cognition of the elderly.
Neurobiol Aging 16: 285-98.
Vitry, S. et al. (2010) Enhanced degradation of synaptophysin by the proteasome in mucopolysaccharidosis type IIIB.
Mol Cell Neurosci. 41: 8-18.
Khan, O.H. et al. (2006) Brain damage in neonatal rats following kaolin induction of hydrocephalus.
Exp Neurol. 200 (2): 311-20.
Del Bigio, M.R. et al. (2003) Chronic hydrocephalus in rats and humans: white matter loss and behavior changes.
Ann Neurol. 53 (3): 337-46.