Synaptophysin antibody | SP15
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Mouse anti Human Synaptophysin
- Product Type
- Monoclonal Antibody
|Mouse anti Human Synaptophysin antibody, clone SP15 recognizes human synaptophysin, also known as Major synaptic vesicle protein p38. Synaptophysin is a 313 amino acid synaptic vesicle associated protein of ~38kDa containing a single marvel domain. Synaptophysin expression is ubiquitous in synaptic vesicle membranes where it is the most abundant protein and is involved in the regulation of endocytosis (Kwon et al. 2011)
Loss and atypical expression of synaptophysin is noted in conditions such as Huntindon's disease (Goto and Hirano 1990) and Alzheimer's disease (Masliah et al. 1989). Mutations in the synaptophysin gene have been identified and lead to a condition, Mental retardation, X-linked 96 (Tarpey et al. 2009).
Mouse anti human synaptophysin antibody, clone SP15 has been used for the detection of synaptophysin by immunofluorescence, immunohistochemistry and western blotting on murine (Vitry et al. 2009), marmoset (Ramirez et al. 2001), rat (Barr et al. 2004), lapine (Roher et al. 2000) and human tissues (Beach et al. 1997).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Hamster Rat Marmoset
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgM - liquid
- Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Crude human synaptic immunoprecipitate.
- Approx. Protein Concentrations
- Isotype concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NSO mouse myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1||1/25|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
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References for Synaptophysin antibody
Honer, W.G. et al. (1994) Hippocampal synaptic pathology in patients with temporal lobe epilepsy.
Acta Neuropathol. 87 (2): 202-10.
Honer, W.G. et al. (1992) Regional synaptic pathology in Alzheimer's disease.
Neurobiol Aging. 13 (3): 375-82.
Honer, W.G. et al. (1993) Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex.
Brain Res. 609: 9-20.
Honer, W.G. et al. (1992) Characterization of a synaptic antigen of interest in neuropsychiatric illness.
Biol Psychiatry. 31 (2): 147-58.
Honer, W.G. et al. (1989) Monoclonal antibodies to study the brain in schizophrenia.
Brain Res. 500 (1-2): 379-83.
Masliah, E. et al. (1994) Topographical distribution of synaptic-associated proteins in the neuritic plaques of Alzheimer's disease hippocampus.
Acta Neuropathol. 87 (2): 135-42.
Del Bigio, M.R. et al. (2003) Chronic hydrocephalus in rats and humans: white matter loss and behavior changes.
Ann Neurol. 53 (3): 337-46.
Wakabayashi, K. et al. (1994) Synapse alterations in the hippocampalentorhinal formation in Alzheimer's disease with and without Lewy body disease.
Brain Research 667: 24-32.
View The Latest Product References
Dickson, D.W. et al. (1994) Hippocampal sclerosis: a common pathological feature of dementia in very old (> or = 80 years of age) humans.
Acta Neuropathol. 88 (3): 212-21.
Dickson, D.W. et al. (1995) Correlations of synaptic and pathological markers with cognition of the elderly.
Neurobiol Aging 16: 285-98.
Vitry, S. et al. (2010) Enhanced degradation of synaptophysin by the proteasome in mucopolysaccharidosis type IIIB.
Mol Cell Neurosci. 41: 8-18.
Khan, O.H. et al. (2006) Brain damage in neonatal rats following kaolin induction of hydrocephalus.
Exp Neurol. 200 (2): 311-20.
Abud, M.B. et al. (2022) In vivo. study to assess dosage of allogeneic pig retinal progenitor cells: Long-term survival, engraftment, differentiation and safety.
J Cell Mol Med. 26(11):3254-68.
- Entrez Gene
- GO Terms
- GO:0005509 calcium ion binding
- GO:0005215 transporter activity
- GO:0015485 cholesterol binding
- GO:0016188 synaptic vesicle maturation
- GO:0019717 synaptosome
- GO:0030054 cell junction
- GO:0030285 integral to synaptic vesicle membrane
- GO:0048169 regulation of long-term neuronal synaptic plasticity
- GO:0048172 regulation of short-term neuronal synaptic plasticity
- View More GO Terms
- GO:0048499 synaptic vesicle membrane organization
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"View all Anti-Human Products
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