SEC61B antibody | rAB01-4H3

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Mouse anti SEC61B

Product Type
PrecisionAb Monoclonal
Clone
rAB01-4H3
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00905
Datasheet
WB
SDS
100 µl loader

Mouse anti SEC61B antibody recognizes protein transport protein Sec61 subunit beta.

SEC61B is a component of the Sec61 translocon (Chiusa et al. 2019), a complex in the endoplasmic reticulum (ER) that mediates membrane insertion of membrane proteins of organelles associated with endocytosis and exocytosis (Lang et al. 2017). The gene encoding SEC61B is associated with polycystic liver diseases, a group of inherited disorders characterized by multiple hepatic cysts (Cornec-Le Gall et al. 2018; Lee-Law et al. 2019).

Product Details

Target Species
Human
Western Blotting
Mouse anti SEC61B antibody detects a band of approximately 12 kDa in HepG2 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Recombinant IgG prepared by affinity chromatography on Protein A from cell culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli--derived recombinant protein of amino acids 2-70 of human SEC61B
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
P60468
Entrez Gene
SEC61B
GO Terms
GO:0000060 protein import into nucleus, translocation
GO:0016021 integral to membrane
GO:0030433 ER-associated protein catabolic process
GO:0030970 retrograde protein transport, ER to cytosol
GO:0031205 endoplasmic reticulum Sec complex
GO:0048408 epidermal growth factor binding
GO:0055085 transmembrane transport
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of SEC61B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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