Browsing Modes for Business Users

IgG antibody | 8A4

ISO 9001 Quality Assurance Manufacturing | Print
Anti Human IgG (Fc) (CH2 Domain) Antibody, clone 8A4 thumbnail image 1
Anti Human IgG (Fc) (CH2 Domain) Antibody, clone 8A4 gallery image 1
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD19 (MCA1940SBV440) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD19 (MCA1940SBV440) and Alexa Fluor 647 conjugated Mouse anti Human IgG (Fc) (CH2 Domain) (MCA2477A647). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human IgG (Fc) (CH2 Domain):Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
8A4
Isotype
IgG1
Specificity
IgG
Region
(Fc) (CH2 DOMAIN)

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
MCA2477A647
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
loader

Mouse anti Human IgG (Fc) (CH2 Domain) antibody, clone 8A4 recognizes an epitope within the CH2 domain of the Fc region of all subclasses of human IgG.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor®647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Human IgG polyclonal.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mouse were fused with cells of the P3-NS1/Ag1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry NT 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
>
List Price Your Price
loader
Description Mouse IgG1 Negative Control:Alexa Fluor® 647

References for IgG antibody

  1. Komatsuda, A. et al. (2008) Monoclonal immunoglobulin deposition disease associated with membranous features.
    Nephrol. Dial. Transplant. 23: 3888-94.
  2. Komatsuda, A. et al. (2013) Proliferative glomerulonephritis with discrete deposition of monoclonal immunoglobulin γ1 C(H) 2 heavy chain and κ light chain: A new variant of monoclonal immunoglobulin deposition disease.
    Pathol Int. 63: 63-7.
  3. Gehlsen, K. et al. (2012) Pharmacokinetics of engineered human monomeric and dimeric CH2 domains.
    MAbs. 4: 466-74.
  4. Kato, H. et al. (2015) Rapid Deterioration of the Renal Function Caused by the Coexistence of Intratubular Amyloidosis and Myeloma Cast Nephropathy.
    Intern Med. 54 (23): 3023-8.

ELISA

UniProt
P01857
P01859
P01860
P01861
Entrez Gene
IGHG1
IGHG2
IGHG3
IGHG4
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response

MCA2477A647

165474

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with IgG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products
Please note