HLA ABC antibody | W6/32

Mouse anti Human HLA ABC:StarBright Violet 790

Product Type
Monoclonal Antibody

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F 100 Tests/0.5ml loader
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Mouse anti Human HLA ABC antibody, clone W6/32 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.

Mouse anti Human HLA ABC antibody, clone W6/32 is routinely tested in flow cytometry on human peripheral blood lymphocytes.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Rhesus Monkey
Guinea Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to StarBright Violet 790 - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Purified human tonsil lymphocyte membranes.
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 790 401 782
For research purposes only
12 months from date of despatch
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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References for HLA ABC antibody

  1. Zuo, J. et al. (2008) The DNase of gammaherpesviruses impairs recognition by virus-specific CD8+ T cells through an additional host shutoff function.
    J Virol. 82: 2385-93.
  2. Raftery, M.J. et al. (2002) Hantavirus infection of dendritic cells.
    J Virol. 76: 10724-33.
  3. Shi, J. et al. (2008) Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
    Blood. 111: 1309-17.
  4. Giuliani, F. et al. (2003) Vulnerability of human neurons to T cell-mediated cytotoxicity.
    J Immunol. 171: 368-79.
  5. Grotzke, J.E. et al. (2009) The Mycobacterium tuberculosis phagosome is a HLA-I processing competent organelle.
    PLoS Pathog. 5: e1000374.
  6. Koch, N. et al. (2009) IL-10 protects monocytes and macrophages from complement-mediated lysis.
    J Leukoc Biol. 86: 155-66.
  7. Smith, A.C. et al. (2005) Interaction of the Salmonella-containing vacuole with the endocytic recycling system.
    J Biol Chem. 280: 24634-41.
  8. Watling, D. et al. (2008) Multiple kinases in the interferon-gamma response.
    Proc Natl Acad Sci U S A. 105: 6051-6.
  9. View The Latest Product References
  10. Lask, A. et al. (2011) TCR-Independent Killing of B Cell Malignancies by Anti-Third-Party CTLs: The Critical Role of MHC-CD8 Engagement.
    J Immunol. 187: 2006-14.
  11. Barnstable, C.J. et al. (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis.
    Cell. 14 (1): 9-20.
  12. Jacobsen, C.N. et al. (1993) Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species.
    Vet Immunol Immunopathol. 39 (4): 461-6.
  13. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49 (2): 97-110.
  14. Neefjes, J.J. et al. (1986) A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes.
    Immunogenetics. 23 (5): 341-7.
  15. Stern, P.L. et al. (1987) Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast.
    J Immunol. 138 (4): 1088-91.
  16. Verbeek, M.M. et al. (1995) T lymphocyte adhesion to human brain pericytes is mediated via very late antigen-4/vascular cell adhesion molecule-1 interactions.
    J Immunol. 154 (11): 5876-84.
  17. Tanabe, M. et al. (1992) Structural and functional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain.
    J Immunol. 148 (10): 3202-9.
  18. Ishitani, A. et al. (2003) Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition.
    J Immunol. 171 (3): 1376-84.
  19. Dressel, R. et al. (2003) Differential effect of acute and permanent heat shock protein 70 overexpression in tumor cells on lysability by cytotoxic T lymphocytes.
    Cancer Res. 63 (23): 8212-20.
  20. Brodsky, F.M. & Parham, P. (1982) Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies.
    Immunogenetics. 15 (2): 151-66.
  21. Hinrichs, J. et al. (2010) The nature of peptides presented by an HLA class I low expression allele.
    Haematologica. 95: 1373-80.
  22. Jones, D.C. et al. (2011) HLA Class I Allelic Sequence and Conformation Regulate Leukocyte Ig-Like Receptor Binding.
    J Immunol. 186: 2990-7.
  23. Spentzou, A. et al. (2010) Viral inhibition assay: a CD8 T cell neutralization assay for use in clinical trials of HIV-1 vaccine candidates.
    J Infect Dis. 201: 720-9.
  24. Fujita, Y. et al. (2010) Bone marrow transplantation restores epidermal basement membrane protein expression and rescues epidermolysis bullosa model mice.
    Proc Natl Acad Sci U S A. 107: 14345-50.
  25. Grotzke, J.E. et al. (2009) The Mycobacterium tuberculosis phagosome is a HLA-I processing competent organelle.
    PLoS Pathog. 5: e1000374.
  26. Narita, M. et al. (2010) WT1 peptide vaccination in combination with imatinib therapy for a patient with CML in the chronic phase.
    Int J Med Sci. 7: 72-81.
  27. Vitadello, M. et al. (2010) Myofiber stress-response in myositis: parallel investigations on patients and experimental animal models of muscle regeneration and systemic inflammation.
    Arthritis Res Ther. 12: R52.
  28. Zuo, J. et al. (2011) The Epstein-Barr virus-encoded BILF1 protein modulates immune recognition of endogenously processed antigen by targeting major histocompatibility complex class I molecules trafficking on both the exocytic and endocytic pathways.
    J Virol. 85: 1604-14.
  29. Enose-Akahata, Y. et al. (2012) Minocycline modulates antigen-specific CTL activity through inactivation of mononuclear phagocytes in patients with HTLV-I associated neurologic disease.
    Retrovirology. 9: 16.
  30. Badrinath, S. et al. (2012) Position 156 influences the peptide repertoire and tapasin dependency of human leukocyte antigen B*44 allotypes.
    Haematologica. 97: 98-106.
  31. Tischer, S. et al. (2016) Discovery of immunodominant T-cell epitopes reveals penton protein as a second immunodominant target in human adenovirus infection.
    J Transl Med. 14 (1): 286.
  32. Dragovic, R.A. et al. (2015) Isolation of syncytiotrophoblast microvesicles and exosomes and their characterisation by multicolour flow cytometry and fluorescence Nanoparticle Tracking Analysis.
    Methods. 87: 64-74.
  33. Praest, P. et al. (2019) A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway.
    Methods Mol Biol. 1988: 187-98.
  34. Tannetta, D.S. et al. (2013) Characterisation of syncytiotrophoblast vesicles in normal pregnancy and pre-eclampsia: expression of Flt-1 and endoglin.
    PLoS One. 8 (2): e56754.
  35. Juan, C.H. et al. (2020) In Vitro Differentiation of Human Placenta-Derived Multipotent Cells into Schwann-Like Cells.
    Biomolecules. 10 (12) Dec 10 [Epub ahead of print].
  36. Tupova, L. et al. (2020) Interplay of drug transporters P-glycoprotein (MDR1), MRP1, OATP1A2 and OATP1B3 in passage of maraviroc across human placenta.
    Biomed Pharmacother. 129: 110506.
  37. Nguyen, J. et al. (2021) Quantitative contributions of TNF receptor superfamily members to CD8+ T-cell responses.
    Mol Syst Biol. 17 (11): e10560.
  38. Cacciola, R. et al. (2022) Impact of Anti-Endothelial Cell Antibodies (AECAs) in Patients with Polycythemia Vera and Thrombosis
    Diagnostics. 12 (5): 1077.
  39. Xia, P. et al. (2022) NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis.
    BMC Immunol. 23 (1): 39.

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