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HLA ABC antibody | W6/32

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Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 1 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 2 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 3 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 4 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 5 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 6 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 7 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 8 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 9 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 10 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 11 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 12 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 13 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 14 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 15 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 16 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 17 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 18 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 19 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 20 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 21 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 22 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 23 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 24 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 25 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 26 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 27 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 28 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 29 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 30 Anti Human HLA ABC Antibody, clone W6/32 thumbnail image 31
Anti Human HLA ABC Antibody, clone W6/32 gallery image 1
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488) and Pacific Blue conjugated Mouse IgG1 isotype control (MCA928PB). Figure B. FITC conjugated Mouse anti Human CD19 (MCA1940F) and Pacific Blue conjugated Mouse anti Human HLA ABC (MCA81PB). All experiments performed on red cell lysed human blood gated on lymphocytes. Data acquired on the ZE5 cell analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 2
Figure A. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and Pacific Blue conjugated Mouse IgG2a isotype control (MCA929PB). Figure B. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and Pacific Blue conjugated Mouse anti Human HLA ABC (MCA81PB). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 3
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488) and RPE-Alexa Fluor 647 conjugated Mouse IgG2a isotype control (MCA929P647). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488) and RPE-Alexa Fluor 647 conjugated Mouse anti Human HLA ABC (MCA81P647). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 4
Published customer image:
Mouse anti Human HLA ABC antibody, clone W6/32 (MCA81R) used to immunoprecipitate all MHC-1 complexes from phagosomal complexes.
Image caption:
HLA-E:peptide complexes are present in phagosomal fractions. (A,B) DC were pulsed with H37Rv-eGFP or CFP102–11 peptide and the homogenate was separated using 27% percoll as described. Each fraction was freeze-thawed and tested for its ability to stimulate D160 1-23 (A) or D160 1-1B (A&B) CD8+ T cell clones in the absence of additional APC. IFN-γ production was measured using ELISPOT. The mean±SEM of duplicate wells is presented. Data are representative of four experiments at various timepoints in A, and four experiments after a one hour peptide pulse in B. (C) Plasma membrane/ER fractions from Mtb–infected DC (HLA-B15+/HLA-B44−) were incubated with HLA-I matched or mismatched CD8+ T cell clones and IFN-γ production was measured using ELISPOT. The mean±SEM of duplicate wells is presented and data are representative of three experiments done similarly. (D,E) Individual fractions were analyzed by flow cytometry to assess HLA-II-PE (plasma membrane) and H37Rv-eGFP fluorescence. Selected fractions are shown (D) including the peak plasma membrane fraction (#8) and phagosomal fractions (#24–27). Prior to flow cytometry, fractions were mixed with a reference latex bead population at a known concentration. Equal numbers of latex bead events were collected for all fractions and used to quantify the number of plasma membrane and phagosome particles (E) as described in Materials and Methods. (F) HLA-I was immunoprecipitated from plasma membrane or phagosome fractions using W6/32. After dilution of the plasma membrane sample to give similar levels of HC10 staining, the presence of HLA-I alleles was assessed using antibodies to pan-HLA-I (HC10), HLA-A2 (HCA2), and HLA-E (MEM-E/02). The numbers below blots indicate the relative intensity of the bands as described in Materials and Methods.
From: Grotzke JE, Harriff MJ, Siler AC, Nolt D, Delepine J, et al. (2009)
The Mycobacterium tuberculosis Phagosome Is a HLA-I Processing Competent Organelle.
PLoS Pathog 5(4): e1000374.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 5
Figure A. Pacific Blue conjugated Mouse anti Human CD3 (MCA463PB). Figure B. Pacific Blue conjugated Mouse anti Human CD3 (MCA463PB) and SBV515 conjugated Mouse anti Human HLA ABC (MCA81SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 6
Published customer image:
FITC conjugated Mouse anti Human HLA ABC antibody, clone W6/32 (MCA81F) used to evaluate HLA-ABC expression on human placenta derived mesenchymal stem cells by flow cytometry.
Image caption:
Phenotypic expression of placenta-derived multipotent stem cells (PDMCs). The results of flow cytometry revealed HLA-ABC+, CD29+, CD44+, CD9+, CD90+, CD105+, SH3+, SH4+, CD166+, CD13+, CD49e+, and CD54+ and HAL-DR-, CD45-, CD34-, Stro-1-, and CD117-. (A,B) markers for MSCs (mesenchymal stem cell) and hematopoietic cells, respectively.

From: Juan CH, Chen MH, Lin FH, Wong CS, Chien CC, Chen MH.
In Vitro Differentiation of Human Placenta-Derived Multipotent Cells into Schwann-Like Cells.
Biomolecules. 2020 Dec 10;10(12):E1657.
doi: 10.3390/biom10121657.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 7
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515) and StarBright Violet 670 conjugated Rat anti Human HLA ABC (MCA81SBV670). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 8
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor conjugated Mouse anti Human CD3 (MCA463A488) and StarBright Violet 440 conjugated Mouse anti Human HLA ABC (MCA81SBV440). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 9
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and StarBright Violet 610 conjugated Mouse anti Human HLA ABC (MCA81SBV610). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 10
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440) and StarBright Violet 570 conjugated Mouse anti Human HLA ABC (MCA81SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 11
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and StarBright Violet 710 conjugated Mouse anti Human HLA ABC (MCA81SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 12
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and StarBright Violet 790 conjugated Mouse anti Human HLA ABC (MCA81SBV790). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 13
Figure A. PE-Cy5 conjugated Mouse anti Human CD3 (MCA463C). Figure B. PE-Cy5 conjugated Mouse anti Human CD3 (MCA463C) and StarBright Violet 475 conjugated Mouse anti Human HLA ABC (MCA81SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 14
Figure A. StarBright Violet 670 conjugated Mouse anti Human CD3 (MCA463SBV670). Figure B. StarBright Violet 670 conjugated Mouse anti Human CD3 (MCA463SBV670) and StarBright UltraViolet 400 conjugated Mouse anti Human HLA ABC (MCA81SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 15
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and StarBright Violet 760 conjugated Mouse anti Human HLA ABC (MCA81SBV760). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 16
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400) and StarBright UltraViolet 665 conjugated Mouse anti Human HLA ABC (MCA81SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 17
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright UltraViolet 445 conjugated Mouse anti Human HLA ABC (MCA81SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 18
Published customer image:
FITC conjugated Mouse anti Human HLA ABC antibody, clone W6/32 (MCA81F) used to illustrate MHC Class I expressing cells in human muscle biopsies by immunofluorescence.
Image caption:
The level of inflammation is increased in the PM patients and the NLRP3/caspase-1/ IL-1β axis is active in their muscle samples. B Immunofluorescence staining showed positive MHC-I staining (grade+ ~ ++) in the PM patients, and negative in the controls (grade−).

From: Xia P, Shao YQ, Yu CC, Xie Y, Zhou ZJ.
NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis.
BMC Immunol. 2022 Aug 14;23(1):39.
doi: 10.1186/s12865-022-00515-2.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 19
Figure A. Pacific Blue conjugated Mouse anti Human CD3 (MCA463PB). Figure B. Pacific Blue conjugated Mouse anti Human CD3 (MCA463PB) and StarBright UltraViolet 795 conjugated Mouse anti human HLA ABC (MCA81SBUV795). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 20
Figure A. PE-Cy5 conjugated Mouse anti Human CD3 (MCA463C). Figure B. PE-Cy5 conjugated Mouse anti Human CD3 (MCA463C) and StarBright UltraViolet 510 conjugated Mouse anti Human HLA ABC (MCA81SBUV510). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 21
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and StarBright UltraViolet 575 conjugated Mouse anti Human HLA ABC (MCA81SBUV575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 22
Figure A. FITC conjugated Mouse anti Human CD3 (MCA463F). Figure B. FITC conjugated Mouse anti Human CD3 (MCA463F) and StarBright UltraViolet 605 conjugated Mouse anti Human HLA ABC (MCA81SBUV605). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 23
Figure A. RPE conjugated Mouse anti Human CD3 (MCA6146PE). Figure B. RPE conjugated Mouse anti Human CD3 (MCA6146PE) and StarBright Blue 580 conjugated Mouse anti Human HLA ABC (MCA81SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 24
Figure A. StarBright Ultra Violet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400). Figure B. StarBright Ultra Violet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400) and StarBright Blue 615 conjugated Mouse anti Human HLA ABC (MCA81SBB615). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 25
Figure A. StarBright Ultra Violet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400). Figure B. StarBright Ultra Violet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400) and StarBright Blue 615 conjugated Mouse anti Human HLA ABC (MCA81SBB615). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 26
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD4 (MCA1267SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD4 (MCA1267SBUV400) and StarBright Blue 675 conjugated Mouse anti Human HLA ABC (MCA81SBB675). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 27
Figure A. APC conjugated Mouse anti Human CD3 (MCA6146APC). Figure B. APC conjugated Mouse anti Human CD3 (MCA6146APC) and StarBright Blue 765 conjugated Mouse anti Human HLA ABC (MCA81SBB765). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 28
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400) and StarBright Blue 810 conjugated Mouse anti Human HLA ABC (MCA81SBB810). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 29
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790) and StarBright Yellow 575 conjugated Mouse anti Human HLA ABC (MCA81SBY575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 30
Figure A. StarBright Ultra Violet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400). Figure B. StarBright Ultra Violet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400) and StarBright Yellow 665 conjugated Mouse anti Human HLA ABC (MCA81SBY665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA ABC Antibody, clone W6/32 gallery image 31
Figure A. StarBright Violet 570 conjugated Mouse anti Human CD3 (MCA463SBV570). Figure B. StarBright Violet 570 conjugated Mouse anti Human CD3 (MCA463SBV570) and StarBright Yellow 800 conjugated Mouse anti Human HLA ABC (MCA81SBY800). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti Human HLA ABC:Pacific Blue®

Product Type
Monoclonal Antibody
Clone
W6/32
Isotype
IgG2a
Specificity
HLA ABC

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MCA81PB
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F 100 Tests/1ml loader
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Mouse anti Human HLA ABC antibody, clone W6/32 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.

Mouse anti Human HLA ABC antibody, clone W6/32 is routinely tested in flow cytometry on human peripheral blood lymphocytes.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Macaque
Bovine
Cynomolgus monkey
Baboon
Rhesus Monkey
Chimpanzee
Gorilla
Shrew
Goat
Dog
Guinea Pig
Rabbit
Mouse
Chicken
Amphibia
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Pacific Blue - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Purified human tonsil lymphocyte membranes.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Pacific Blue® 410 455
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:Pacific Blue® MCA929PB F 100 Tests/1ml loader
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Description Mouse IgG2a Negative Control:Pacific Blue®

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Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
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Description Human Seroblock

Source Reference

  1. Barnstable, C.J. et al. (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis.
    Cell. 14 (1): 9-20.

References for HLA ABC antibody

  1. Brodsky, F.M. & Parham, P. (1982) Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies.
    Immunogenetics. 15 (2): 151-66.
  2. Neefjes, J.J. et al. (1986) A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes.
    Immunogenetics. 23 (5): 341-7.
  3. Stern, P.L. et al. (1987) Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast.
    J Immunol. 138 (4): 1088-91.
  4. Jacobsen, C.N. et al. (1993) Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species.
    Vet Immunol Immunopathol. 39 (4): 461-6.
  5. Verbeek, M.M. et al. (1995) T lymphocyte adhesion to human brain pericytes is mediated via very late antigen-4/vascular cell adhesion molecule-1 interactions.
    J Immunol. 154 (11): 5876-84.
  6. Raftery, M.J. et al. (2002) Hantavirus infection of dendritic cells.
    J Virol. 76: 10724-33.
  7. Dressel, R. et al. (2003) Differential effect of acute and permanent heat shock protein 70 overexpression in tumor cells on lysability by cytotoxic T lymphocytes.
    Cancer Res. 63 (23): 8212-20.
  8. Ishitani, A. et al. (2003) Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition.
    J Immunol. 171 (3): 1376-84.
    9. View The Latest Product References
  9. Giuliani, F. et al. (2003) Vulnerability of human neurons to T cell-mediated cytotoxicity.
    J Immunol. 171: 368-79.
  10. Smith, A.C. et al. (2005) Interaction of the Salmonella-containing vacuole with the endocytic recycling system.
    J Biol Chem. 280: 24634-41.
  11. Zuo, J. et al. (2008) The DNase of gammaherpesviruses impairs recognition by virus-specific CD8+ T cells through an additional host shutoff function.
    J Virol. 82: 2385-93.
  12. Shi, J. et al. (2008) Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
    Blood. 111: 1309-17.
  13. Watling, D. et al. (2008) Multiple kinases in the interferon-gamma response.
    Proc Natl Acad Sci U S A. 105: 6051-6.
  14. Grotzke, J.E. et al. (2009) The Mycobacterium tuberculosis phagosome is a HLA-I processing competent organelle.
    PLoS Pathog. 5: e1000374.
  15. Koch, N. et al. (2009) IL-10 protects monocytes and macrophages from complement-mediated lysis.
    J Leukoc Biol. 86: 155-66.
  16. Spentzou, A. et al. (2010) Viral inhibition assay: a CD8 T cell neutralization assay for use in clinical trials of HIV-1 vaccine candidates.
    J Infect Dis. 201: 720-9.
  17. Vitadello, M. et al. (2010) Myofiber stress-response in myositis: parallel investigations on patients and experimental animal models of muscle regeneration and systemic inflammation.
    Arthritis Res Ther. 12: R52.
  18. Hinrichs, J. et al. (2010) The nature of peptides presented by an HLA class I low expression allele.
    Haematologica. 95: 1373-80.
  19. Fujita, Y. et al. (2010) Bone marrow transplantation restores epidermal basement membrane protein expression and rescues epidermolysis bullosa model mice.
    Proc Natl Acad Sci U S A. 107: 14345-50.
  20. Narita, M. et al. (2010) WT1 peptide vaccination in combination with imatinib therapy for a patient with CML in the chronic phase.
    Int J Med Sci. 7: 72-81.
  21. Zuo, J. et al. (2011) The Epstein-Barr virus-encoded BILF1 protein modulates immune recognition of endogenously processed antigen by targeting major histocompatibility complex class I molecules trafficking on both the exocytic and endocytic pathways.
    J Virol. 85: 1604-14.
  22. Lask, A. et al. (2011) TCR-Independent Killing of B Cell Malignancies by Anti-Third-Party CTLs: The Critical Role of MHC-CD8 Engagement.
    J Immunol. 187: 2006-14.
  23. Jones, D.C. et al. (2011) HLA Class I Allelic Sequence and Conformation Regulate Leukocyte Ig-Like Receptor Binding.
    J Immunol. 186: 2990-7.
  24. Enose-Akahata, Y. et al. (2012) Minocycline modulates antigen-specific CTL activity through inactivation of mononuclear phagocytes in patients with HTLV-I associated neurologic disease.
    Retrovirology. 9: 16.
  25. Badrinath, S. et al. (2012) Position 156 influences the peptide repertoire and tapasin dependency of human leukocyte antigen B*44 allotypes.
    Haematologica. 97: 98-106.
  26. Tannetta, D.S. et al. (2013) Characterisation of syncytiotrophoblast vesicles in normal pregnancy and pre-eclampsia: expression of Flt-1 and endoglin.
    PLoS One. 8 (2): e56754.
  27. Dragovic, R.A. et al. (2015) Isolation of syncytiotrophoblast microvesicles and exosomes and their characterisation by multicolour flow cytometry and fluorescence Nanoparticle Tracking Analysis.
    Methods. 87: 64-74.
  28. Tischer, S. et al. (2016) Discovery of immunodominant T-cell epitopes reveals penton protein as a second immunodominant target in human adenovirus infection.
    J Transl Med. 14 (1): 286.
  29. Praest, P. et al. (2019) A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway.
    Methods Mol Biol. 1988: 187-98.
  30. Juan, C.H. et al. (2020) In Vitro Differentiation of Human Placenta-Derived Multipotent Cells into Schwann-Like Cells.
    Biomolecules. 10 (12): 1657.
  31. Tupova, L. et al. (2020) Interplay of drug transporters P-glycoprotein (MDR1), MRP1, OATP1A2 and OATP1B3 in passage of maraviroc across human placenta.
    Biomed Pharmacother. 129: 110506.
  32. Nguyen, J. et al. (2021) Quantitative contributions of TNF receptor superfamily members to CD8+ T-cell responses.
    Mol Syst Biol. 17 (11): e10560.
  33. Xia, P. et al. (2022) NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis.
    BMC Immunol. 23 (1): 39.
  34. Cacciola, R. et al. (2022) Impact of Anti-Endothelial Cell Antibodies (AECAs) in Patients with Polycythemia Vera and Thrombosis
    Diagnostics. 12 (5): 1077.
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    Metabolites.12 (10): 924.

Flow Cytometry

Functional Assays

Immunofluorescence

MCA81PB

1611

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