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Fibroblasts/Epithelial Cells antibody | D7-FIB

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Anti Human Fibroblasts/Epithelial Cells Antibody, clone D7-FIB thumbnail image 1 Anti Human Fibroblasts/Epithelial Cells Antibody, clone D7-FIB thumbnail image 2
Anti Human Fibroblasts/Epithelial Cells Antibody, clone D7-FIB gallery image 1
Flow cytometry staining of KG1 cells with Mouse anti Human Fibroblasts/ Epithelial cells (MCA1399G).
Anti Human Fibroblasts/Epithelial Cells Antibody, clone D7-FIB gallery image 2
Published customer image:
Mouse anti Human fibroblasts/ epithelial cells antibody, clone D7-FIB (MCA1399G) used to demonstrate fibroblasts in micromasses by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Composition of micromasses constructed from primary RA FLS and CD14+ PBMCs CD68 staining of macrophages in the micromass. Black arrows indicate CD68+ cells and grey arrows indicate CD68-negative cells. 11-Fibrau staining of FLS in the micromass. Black arrows indicate 11-Fibrau+ cells and grey arrows indicate 11-Fibrau-negative cells.

From: Broeren MGA, Waterborg CEJ, Wiegertjes R, Thurlings RM, Koenders MI, Van Lent PLEM, Van der Kraan PM, Van de Loo FAJ.
A three-dimensional model to study human synovial pathology.
ALTEX. 2019;36(1):18-28.
doi: 10.14573/altex.1804161.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human Fibroblasts/Epithelial Cells:RPE

Product Type
Monoclonal Antibody
Clone
D7-FIB
Isotype
IgG2a
Specificity
Fibroblasts/Epithelial Cells

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Product Code Applications Pack Size List Price Your Price Qty
MCA1399PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
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Mouse anti Human fibroblasts/epithelial cells antibody, clone D7-FIB recognizes a ~112kDa molecule expressed on the cell surface of human fibroblasts. The antigen is not expressed by peripheral blood cells, and is found at low levels on a minority of melanoma cell lines. The epitope recognized by clone D7-FIB is sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase.

Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB also binds to epithelium, myoepthelium, smooth muscle and some leucocytes.

Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (van Osch et al. 2001).

Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is routinely tested in flow cytometry on the KG1 cell line.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Human foreskin fibroblasts.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
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  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

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References for Fibroblasts/Epithelial Cells antibody

  1. Fearns C & Dowdle EB (1992) The desmoplastic response: induction of collagen synthesis by melanoma cells in vitro.
    Int J Cancer. 50 (4): 621-7.
  2. Jayne, D.G. et al. (1999) A three-dimensional in-vitro model for the study of peritoneal tumour metastasis.
    Clin Exp Metastasis. 17 (6): 515-23.
  3. Kelynack, K.J. et al. (2000) Human renal fibroblast contraction of collagen I lattices is an integrin-mediated process.
    Nephrol Dial Transplant. 15 (11): 1766-72.
  4. Todisco, E. et al. (2002) CD40 ligand-stimulated B cell precursor leukemic cells elicit interferon-gamma production by autologous bone marrow T cells in childhood acute lymphoblastic leukemia.
    Leukemia. 16 (10): 2046-54.
  5. Jones. E.A. et al. (2002) Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells.
    Arthritis. Rheum. 46: 3349-60.
  6. Telfer, J.F. and Brock, J.H. (2002) Expression of ferritin, transferrin receptor, and non-specific resistance associated macrophage proteins 1 and 2 (Nramp1 and Nramp2) in the human rheumatoid synovium.
    Ann Rheum Dis. 61: 741-4.
  7. Petrow, P.K. et al. (2002) Characterisation of the cell type-specificity of collagenase 3 mRNA expression in comparison with membrane type 1 matrix metalloproteinase and gelatinase A in the synovial membrane in rheumatoid arthritis.
    Ann Rheum Dis. 61: 391-7.
  8. Pap, T. et al. (2003) Osteoclast-independent bone resorption by fibroblast-like cells
    Arthritis Res Ther. 5: R163-73.
    9. View The Latest Product References
  9. Miranda-Carús, M.E. et al. (2004) Rheumatoid arthritis synovial fluid fibroblasts express TRAIL-R2 (DR5) that is functionally active.
    Arthritis Rheum. 50: 2786-93.
  10. Manoussaka, M.S. et al. (2005) Flow cytometric characterisation of cells of differing densities isolated from human term placentae and enrichment of villous trophoblast cells.
    Placenta. 26 (4): 308-18.
  11. De Bari, C. et al. (2006) Mesenchymal multipotency of adult human periosteal cells demonstrated by single-cell lineage analysis.
    Arthritis Rheum. 54 (4): 1209-21.
  12. Jones, E.A. et al. (2006) Optimization of a flow cytometry-based protocol for detection and phenotypic characterization of multipotent mesenchymal stromal cells from human bone marrow.
    Cytometry B Clin Cytom. 70: 391-9.
  13. English, A. et al. (2007) A comparative assessment of cartilage and joint fat pad as a potential source of cells for autologous therapy development in knee osteoarthritis.
    Rheumatology (Oxford). 46: 1676-83.
  14. Martinez, C. et al. (2007) Human bone marrow mesenchymal stromal cells express the neural ganglioside GD2: a novel surface marker for the identification of MSCs.
    Blood. 109 (10): 4245-8.
  15. Morito, T. et al. (2008) Synovial fluid-derived mesenchymal stem cells increase after intra-articular ligament injury in humans.
    Rheumatology (Oxford). 47 (8): 1137-43.
  16. Nimura, A. et al. (2008) Increased proliferation of human synovial mesenchymal stem cells with autologous human serum: comparisons with bone marrow mesenchymal stem cells and with fetal bovine serum.
    Arthritis Rheum. 58: 501-10.
  17. Scutt, N. et al. (2008) Tissue specific characteristics of cells isolated from human and rat tendons and ligaments.
    J Orthop Surg Res. 3: 32.
  18. De Bari, C. et al. (2008) A biomarker-based mathematical model to predict bone-forming potency of human synovial and periosteal mesenchymal stem cells.
    Arthritis Rheum. 58 (1): 240-50.
  19. Kanayama, M. et al. (2009) Alpha9 integrin and its ligands constitute critical joint microenvironments for development of autoimmune arthritis.
    J Immunol. 182: 8015-25.
  20. Pountos, I. et al. (2011) NSAIDS inhibit in vitro MSC chondrogenesis but not osteogenesis: implications for mechanism of bone formation inhibition in man.
    J Cell Mol Med. 15: 525-34.
  21. Sekiya, I. et al. (2012) Human mesenchymal stem cells in synovial fluid increase in the knee with degenerated cartilage and osteoarthritis.
    J Orthop Res. 30: 943-9.
  22. Behl, B. et al. (2013) Biological effects of cobalt-chromium nanoparticles and ions on dural fibroblasts and dural epithelial cells.
    Biomaterials. 34 (14): 3547-58.
  23. Asano, T. et al. (2014) α9β1 integrin acts as a critical intrinsic regulator of human rheumatoid arthritis.
    Rheumatology (Oxford). 53 (3): 415-24.
  24. Papageorgiou, I. et al. (2014) Interaction of micron and nano-sized particles with cells of the dura mater.
    J Biomed Mater Res B Appl Biomater. 102 (7): 1496-505.
  25. Iyyanki, T. et al. (2015) Harvesting technique affects adipose-derived stem cell yield.
    Aesthet Surg J. 35 (4): 467-76.
  26. Schminke, B. et al. (2015) The pathology of bone tissue during peri-implantitis.
    J Dent Res. 94 (2): 354-61.
  27. Grognuz, A. et al. (2016) Human Fetal Progenitor Tenocytes for Regenerative Medicine.
    Cell Transplant. 25 (3): 463-79.
  28. Broeren, M.G.A. (2019) A three-dimensional model to study human synovial pathology
    ALTEX. 36 (1): 18-28.

Flow Cytometry

Immunohistology - Paraffin

RRID
AB_1055724

MCA1399PE

150141 157597 159129

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