EPS15 antibody | CD01/1C2
IP-MS Validated
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WB IP ResetMouse anti EPS15
- Product Type
- PrecisionAb Monoclonal
- Clone
- CD01/1C2
- Isotype
- IgG1
- Format
- Purified
- Specificity
- EPS15
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| Mouse anti EPS15 antibody recognizes epidermal growth factor receptor substrate 15 (EPS15), also known as protein AF-1p. EPS15 is an endocytic accessory protein which plays a role in the internalization of ligand-indicible receptors, notably EGFR, by interacting with the clathrin assembly adaptors AP2 and EPSIN1 (Milesi et al. 2019). EGFR activation induces sequential EPS15 phosphorylation, activating translocation of EPS15 from endosomes to the cytosol. The C-terminus of EPS15 contains two ubiqitin-interacting motifs, which can bind EGFR directly (Haugen et al. 2017). Because of its role in terminating signaling from growth factors, EPS15 is involved with several malignancies. Overexpression of EPS15 has been found to be associated with a favourable clinical outcome in patients with breast cancer (Dai and Zhang 2015). |
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- Target Species
- Human
- Western Blotting
- Mouse anti EPS15 antibody detects a band of approximately 135 kDa in HeLa cell lysates
- Species Cross-Reactivity
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Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli-derived recombinant protein, aa 492-896 of human EPS15
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation |  |
||
| Western Blotting | |
1/1000 | 1/10,000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
- UniProt
- P42566
- Entrez Gene
- EPS15
- GO Terms
- GO:0005886 plasma membrane
- GO:0005509 calcium ion binding
- GO:0005829 cytosol
- GO:0005905 coated pit
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0008283 cell proliferation
- GO:0015031 protein transport
- GO:0017124 SH3 domain binding
- GO:0031901 early endosome membrane
- View More GO Terms
- GO:0032456 endocytic recycling
- GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
- GO:0048268 clathrin coat assembly
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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