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EMR3 antibody | 3D7

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Hamster anti Human EMR3:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
3D7
Isotype
IgG
Specificity
EMR3

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Product Code Applications Pack Size List Price Your Price Qty
MCA2476A647
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SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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Hamster anti Human EMR3 antibody, clone 3D7 recognizes human epidermal growth factor (EGF) module-containing mucin-like hormone receptor 3 (EMR3), a ~56 kDa member of the EGF-7 transmembrane (TM7) family of adhesion receptors. EMR3 is expressed at the cell surface as a heterodimer.

EMR3 is predominantly expressed on granulocytes, and at lower levels on mature myeloid cells, monocytes and dendritic cells. EMR3 is absent on lymphocytes, haematopoietic stem cells and myeloid progenitors. EMR3 is up-regulated during late stages of neutrophil differentiation and is a marker for terminally differentiated cells (Matmati et al.2007).

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen
ARHO-EMR3-CD97 (EGF1) transfectants.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized Armenian hamster were fused with cells of the mouse SP2/0 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control:Alexa Fluor® 647 MCA2356A647 F 100 Tests/1ml loader
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List Price Your Price
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Description Hamster (Armenian) IgG Negative Control:Alexa Fluor® 647

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

Source Reference

  1. Matmati, M. et al. (2007) The human EGF-TM7 receptor EMR3 is a marker for mature granulocytes.
    J Leukoc Biol. 81 (2): 440-8.

References for EMR3 antibody

  1. Drewniak, A. etal. (2009) Changes in gene expression of granulocytes during in vivo granulocyte colony-stimulating factor/dexamethasone mobilization for transfusion purposes.
    Blood. 113: 5979-98.
  2. Gazendam, R.P. et al. (2016) Impaired killing of Candida albicans. by granulocytes mobilized for transfusion purposes: a role for granule components.
    Haematologica. 101 (5): 587-96.
  3. van de Geer, A. et al. (2017) Characterization of buffy coat-derived granulocytes for clinical use: a comparison with granulocyte colony-stimulating factor/dexamethasone-pretreated donor-derived products.
    Vox Sang. 112 (2): 173-82.
  4. Hansen, M. et al. (2018) Efficient production of erythroid, megakaryocytic and myeloid cells, using single cell-derived iPSC colony differentiation.
    Stem Cell Res. 29: 232-44.

Further Reading

  1. Carpenter, G. & Cohen, S. (1990) Epidermal growth factor.
    J Biol Chem. 265 (14): 7709-12.

RRID
AB_872001
UniProt
Q9BY15
Entrez Gene
EMR3
GO Terms
GO:0005886 plasma membrane
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0004930 G-protein coupled receptor activity
GO:0005615 extracellular space
GO:0007218 neuropeptide signaling pathway

MCA2476A647

150514

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