DNA antibody | AC-30-10

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Mouse anti DNA

Product Type
Monoclonal Antibody
Clone
AC-30-10
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0200-0613 C IB IF P 50 µg
Mouse anti DNA antibody, clone AC-30-10 reacts with all forms of native and denatured DNA, in all DNA containing species tested so far. It also reacts with lambda DNA and synthetic DNA. It has also been found to react with the following cell lines, XLKE-A6, RVF-SMC, PTK-2, and HeLa.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken
Mouse
Amphibia
Insect
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by gel filtration.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide, 0.5% Bovine Serum Albumin.
Immunogen
Human DNA.
Approx. Protein Concentrations
IgM concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of DNA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoblotting
Immunofluorescence
Immunohistology - Frozen 1/10
Immunohistology - Paraffin 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Product Specific References

References for DNA antibody

  1. Scheer, U. et al. (1987) High sensitivity immunolocalization of double and single-stranded DNA by a monoclonal antibody.
    Eur J Cell Biol. 43: 358-71.
  2. Hansmann, P. et al. (1986) Ultrastructural localization of DNA in two Cryptomonas species by use of a monoclonal DNA antibody
    Eur J Cell Biol. 42: 152-60