DNA antibody | AC-30-10

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Mouse anti DNA

Product Type: Monoclonal Antibody
Clone: AC-30-10
Isotype: IgM
Specificity: DNA

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Secondary Antibodies
References (2)
Applications
Storage Information

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0200-0613 Datasheet Datasheet SDS SDS	C IB IF P	50 µg		Log in	Get Quote
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Mouse anti DNA antibody, clone AC-30-10 reacts with all forms of native and denatured DNA, in all DNA containing species tested so far. It also reacts with lambda DNA and synthetic DNA. It has also been found to react with the following cell lines, XLKE-A6, RVF-SMC, PTK-2, and HeLa.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Chicken

Mouse

Amphibia Expected from Sequence

Insect Expected from Sequence

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgM - liquid
Preparation: Purified IgM prepared by gel filtration from ascites
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
0.05% Bovine Serum Albumin
Immunogen: Human DNA.
Approx. Protein Concentrations: IgM concentration 0.1 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Chicken
Mouse
Amphibia	Expected from Sequence
Insect	Expected from Sequence
Rat

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoblotting
Immunofluorescence
Immunohistology - Frozen			1/10
Immunohistology - Paraffin			1/10

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for DNA antibody

Scheer, U. et al. (1987) High sensitivity immunolocalization of double and single-stranded DNA by a monoclonal antibody.
Eur J Cell Biol. 43: 358-71.
Hansmann, P. et al. (1986) Ultrastructural localization of DNA in two Cryptomonas species by use of a monoclonal DNA antibody
Eur J Cell Biol. 42: 152-60