DNA antibody | AC-30-10

Mouse anti DNA

Product Type
Monoclonal Antibody
Clone
AC-30-10
Isotype
IgM
Specificity
DNA

Product Code Applications Pack Size List Price Your Price Qty
0200-0613
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C IB IF P 50 µg loader
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loader
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Mouse anti DNA antibody, clone AC-30-10 reacts with all forms of native and denatured DNA, in all DNA containing species tested so far. It also reacts with lambda DNA and synthetic DNA. It has also been found to react with the following cell lines, XLKE-A6, RVF-SMC, PTK-2, and HeLa.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken
Mouse
Amphibia Expected from Sequence
Insect Expected from Sequence
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by gel filtration from ascites
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.05% Bovine Serum Albumin
Immunogen
Human DNA.
Approx. Protein Concentrations
IgM concentration 0.1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoblotting
Immunofluorescence
Immunohistology - Frozen 1/10
Immunohistology - Paraffin 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for DNA antibody

  1. Scheer, U. et al. (1987) High sensitivity immunolocalization of double and single-stranded DNA by a monoclonal antibody.
    Eur J Cell Biol. 43: 358-71.
  2. Hansmann, P. et al. (1986) Ultrastructural localization of DNA in two Cryptomonas species by use of a monoclonal DNA antibody
    Eur J Cell Biol. 42: 152-60

RRID
AB_2092752
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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