DNA antibody | AC-30-10
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Mouse anti DNA antibody, clone AC-30-10 reacts with all forms of native and denatured DNA, in all DNA containing species tested so far. It also reacts with lambda DNA and synthetic DNA. It has also been found to react with the following cell lines, XLKE-A6, RVF-SMC, PTK-2, and HeLa.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chicken Mouse Amphibia Expected from Sequence Insect Expected from Sequence Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgM - liquid
- Preparation
- Purified IgM prepared by gel filtration.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.05% Bovine Serum Albumin - Immunogen
- Human DNA.
- Approx. Protein Concentrations
- IgM concentration 0.1 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of DNA antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoblotting | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | 1/10 | ||
| Immunohistology - Paraffin | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti Mouse IgM:FITC | HCA040F | F | 0.1 mg |
|
|
| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | C E P WB | 1 ml |
|
|
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | C E WB | 1 mg |
|
|
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg |
|
Product Specific References
References for DNA antibody
-
Scheer, U. et al. (1987) High sensitivity immunolocalization of double and single-stranded DNA by a monoclonal antibody.
Eur J Cell Biol. 43: 358-71. -
Hansmann, P. et al. (1986) Ultrastructural localization of DNA in two Cryptomonas species by use of a monoclonal DNA antibody
Eur J Cell Biol. 42: 152-60
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
