DJ-1 antibody | AbD03055

Human anti Human DJ-1 (Oxidized at C106)

Product Type
Monoclonal Antibody
Clone
AbD03055
Isotype
HuCAL Fab bivalent
Specificity
DJ-1
Region
(OXIDIZED AT C106)

Product Code Applications Pack Size List Price Your Price Qty
HCA024
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SDS Safety Datasheet SDS
E WB * 0.1 mg loader
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loader
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Human anti Human DJ-1 antibody, clone AbD03055 recognizes the human DJ-1 oncogene only when it is oxidized on cysteine C106. DJ-1 plays roles in transcriptional regulation and reaction to anti-oxidative stress. The oxidation status of DJ-1, particularly at C106, modulates the function of DJ-1 (Canet-Avilés et al. 2004). De-regulation of DJ-1 oxidation appears to be connected to the onset of conditions such as Parkinson's Disease (Abou-Sleiman et al. 2003).

Target Species
Human
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
DJ-1 peptide oxidized at C106 (sequence LIAAIC(SO3)AGPTA).
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
By purchasing this product, you agree that your Name and Affiliation will be sent to Dr. Ariga, Hokkaido University, who first published this antibody in the journal Neuroscience Letters (Vol. 404, pp.166-169, 2006).
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1
  1. 1Detection via the Histidine Tag is recommended for Western Blotting applications. Bio-Rad suggest product MCA5995P for this purpose
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader
List Price Your Price
loader
Description Mouse anti Penta Histidine Tag:HRP

References for DJ-1 antibody

  1. Ooe, H. et al. (2006) Establishment of specific antibodies that recognize C106-oxidized DJ-1.
    Neurosci Lett. 404 (1-2): 166-9.
  2. Shadrach KG et al. (2013) DJ-1-dependent regulation of oxidative stress in the retinal pigment epithelium (RPE).
    PLoS One. 8 (7): e67983.
  3. Andres-Mateos, E. et al. (2007) DJ-1 gene deletion reveals that DJ-1 is an atypical peroxiredoxin-like peroxidase.
    Proc Natl Acad Sci U S A. 104 (37): 14807-12.
  4. Bitar, M.S. et al. (2012) Decline in DJ-1 and decreased nuclear translocation of Nrf2 in Fuchs endothelial corneal dystrophy.
    Invest Ophthalmol Vis Sci. 53 (9): 5806-13.
  5. Zhang C et al. (2008) Role of NonO-histone interaction in TNFalpha-suppressed prolyl-4-hydroxylase alpha1.
    Biochim Biophys Acta. 1783 (8): 1517-28.
  6. Kim, J. et al. (2015) DJ-1 protects against undernutrition-induced atrophy through inhibition of the MAPK-ubiquitin ligase pathway in myoblasts.
    Life Sci. 143: 50-7.

Western Blotting

Synonyms
PARK7
RRID
AB_2160114
UniProt
Q99497
Entrez Gene
PARK7
GO Terms
GO:0005515 protein binding
GO:0005634 nucleus
GO:0008219 cell death
GO:0032091 negative regulation of protein binding
GO:0060765 regulation of androgen receptor signaling pathway
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
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