CDC42 antibody | 16C2-C10

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Mouse anti CDC42

Product Type
PrecisionAb Monoclonal
Clone
16C2-C10
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00871
Datasheet
WB
SDS
100 µl loader

Mouse anti CDC42 antibody recognizes cell division control protein 42 homolog (CDC42), also known as G25K GTP-binding protein.

CDC42 is a small ubiquitously-expressed GTPase of the Rho family and has been implicated in a range of cellular processes including cytoskeleton organization and membrane trafficking, and achieves this by regulating multiple signaling pathways. It cycles between an inactive GDP-bound state and an active GTP-bound state through exchange of GDP for GTP (Arias-Romero and Chernoff 2013). During development, CDC42 is important for formation of the pancreas, blood cells, eyes, and skin (Xiao et al. 2018). CDC42 has been closely associated with cancer, including processes of cell cycle progression, migration and invasion, tumor growth, angiogenesis and transformation (del Mar Maldonado and Dharmawardhane 2018). Abnormal CDC42 expression has been found in most human cancers and acts to promote metastasis (Xiao et al. 2018).

Mouse anti CDC24 antibody recognizes epitope sequence corresponding to amino acids 137 to 142 of CDC24.

Product Details

Target Species
Human
Western Blotting
Anti CDC42 detects a band of approximately 22 kDa in HEK293 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to CDC42 amino acids 121 to 150
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P60953
Entrez Gene
CDC42
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0003924 GTPase activity
GO:0030036 actin cytoskeleton organization
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0005525 GTP binding
GO:0007163 establishment or maintenance of cell polarity
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007264 small GTPase mediated signal transduction
GO:0007411 axon guidance
GO:0030175 filopodium
GO:0030225 macrophage differentiation
GO:0031274 positive regulation of pseudopodium assembly
GO:0031295 T cell costimulation
GO:0031333 negative regulation of protein complex assembly
GO:0031996 thioesterase binding
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
GO:0042692 muscle cell differentiation
GO:0051056 regulation of small GTPase mediated signal transduction
GO:0051149 positive regulation of muscle cell differentiation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CDC42 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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