CD9 antibody | MM2/57
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|Mouse anti Human CD9 antibody, clone MM2/57 recognizes human leukocyte antigen MIC3 also known as MRP-1 or CD9. CD9 is a 228 amino acid multi pass membrane glycoprotein belonging to the tetraspanin family with a molecular weight of ~24 kDa expressed by platelets, monocytes, some lymphocytes and endothelial cells.
Mouse anti Human CD9 antibody, clone MM2/57 recognizes a conserved epitope on CD9 present on a wide range of mammalian species.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Cat Rhesus Monkey Bovine Dog Rabbit Horse Pig Mustelid Expected from Sequence Mink Llama Ferret
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin
- Human platelet membranes
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells from the SP2/0 mouse myeloma line
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519
- For research purposes only
- 12 months from date of despatch
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or email@example.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
References for CD9 antibody
Ed Knapp W. et al. (1989) Leucocyte Typing IV
Oxford University Press.
Boucheix, C. et al. (1991) Molecular cloning of the CD9 antigen. A new family of cell surface proteins.
J Biol Chem. 266 (1): 117-22.
Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
Vet Immunol Immunopathol. 64 (1): 1-13.
Ibrahim,S. et al. (2007) Screening of anti-human leukocyte monoclonal antibodies for reactivity with equine leukocytes
Vet.Immunol Immunopathol. 119: 63-80
Jennings, L. K. et al. (1995) CD9 cluster workshop report: cell surface binding and functional analysis.
In S.F. Sclossman. et al. Editors. 1995. Leucocyte Typing V. White Cell Differentiation Antigens. Oxford University Press, New York, NY. 1249-1251.
Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15.
Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
Vet Immunol Immunopathol. 119: 27-37.
Davis, W.C. et al. (2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.
Vet Immunol Immunopathol. 119: 123-30.
View The Latest Product References
Ferrer, M. et al. (1998) Pattern of expression of tetraspanin antigen genes in Burkitt lymphoma cell lines.
Clin Exp Immunol. 113: 346-52.
Kao, Y.R. et al. (2003) Tumor-associated antigen L6 and the invasion of human lung cancer cells.
Clin Cancer Res. 9: 2807-16.
Müller, T. et al. (2009) A novel embryonic stem cell line derived from the common marmoset monkey (Callithrix jacchus) exhibiting germ cell-like characteristics.
Hum Reprod. 24: 1359-72.
Kubota, H. et al. (2011) Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
FASEB J. 25 (8): 2604-14.
Hogue, I.B. et al. (2011) Gag induces the coalescence of clustered lipid rafts and tetraspanin-enriched microdomains at HIV-1 assembly sites on the plasma membrane.
J Virol. 85 (19): 9749-66.
Löffler, S. et al. (1997) CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus.
J Virol. 71: 42-9.
Meister, R.K. et al. (2007) Progress in the discovery and definition of monoclonal antibodies for use in feline research.
Vet Immunol Immunopathol. 119: 38-46.
Bearden, R.N. et al. (2017) In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
Stem Cell Res Ther. 8 (1): 218.
Jackson, C.E. et al. (2017) Effects of Inhibiting VPS4 Support a General Role for ESCRTs in Extracellular Vesicle Biogenesis.
Biophys J. 113 (6): 1342-1352.
Wąchalska, M. et al. (2020) Palmitoylated mNeonGreen Protein as a Tool for Visualization and Uptake Studies of Extracellular Vesicles
Membranes. 10 (12): 373.
Fish, E.J. et al. (2018) Malignant canine mammary epithelial cells shed exosomes containing differentially expressed microRNA that regulate oncogenic networks.
BMC Cancer. 18 (1): 832.
Fu, T. et al. (2021) Biomimetic vascularized adipose-derived mesenchymal stem cells bone-periosteum graft enhances angiogenesis and osteogenesis in a rabbit spine fusion model.
Stem Cell Res Therapy.15 Sep [Epub ahead of print].
Viswanathan, K. et al. (2017) Quantitative membrane proteomics reveals a role for tetraspanin enriched microdomains during entry of human cytomegalovirus.
PLoS One. 12 (11): e0187899.
- Entrez Gene
- GO Terms
- GO:0005515 protein binding
- GO:0002576 platelet degranulation
- GO:0007155 cell adhesion
- GO:0005887 integral to plasma membrane
- GO:0006928 cellular component movement
- GO:0007342 fusion of sperm to egg plasma membrane
- GO:0030168 platelet activation
- GO:0030913 paranodal junction assembly
- GO:0031092 platelet alpha granule membrane
- View More GO Terms
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