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CD9 antibody | MM2/57

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Anti Human CD9 Antibody, clone MM2/57 thumbnail image 1 Anti Human CD9 Antibody, clone MM2/57 thumbnail image 2 Anti Human CD9 Antibody, clone MM2/57 thumbnail image 3 Anti Human CD9 Antibody, clone MM2/57 thumbnail image 4 Anti Human CD9 Antibody, clone MM2/57 thumbnail image 5 Anti Human CD9 Antibody, clone MM2/57 thumbnail image 6 Anti Human CD9 Antibody, clone MM2/57 thumbnail image 7 Anti Human CD9 Antibody, clone MM2/57 thumbnail image 8 Anti Human CD9 Antibody, clone MM2/57 thumbnail image 9
Anti Human CD9 Antibody, clone MM2/57 gallery image 1
Figure A. FITC conjugated Mouse anti Human CD61 (MCA2588F) and Alexa Fluor 700 conjugated Mouse IgG2b isotype control (MCA691A700). Figure B. FITC conjugated Mouse anti Human CD61 (MCA2588F) and Alexa Fluor 700 conjugated Mouse anti Human CD9 (MCA469A700). All experiments performed on human blood gated on live single platelets, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD9 Antibody, clone MM2/57 gallery image 2
Figure A. FITC conjugated Mouse anti Human CD14 (MCA596F) and RPE conjugated Mouse IgG2b isotype control (MCA691PE). Figure B. FITC conjugated Mouse anti Human CD14 (MCA596F) and RPE conjugated Mouse anti Human CD9 (MCA469PE). All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A)
Anti Human CD9 Antibody, clone MM2/57 gallery image 3
Staining of acetone fixed frozen human tonsil with Mouse anti Human CD9 (MCA469GA).
Anti Human CD9 Antibody, clone MM2/57 gallery image 4
Published customer image:
Mouse anti Human CD9 antibody, clone MM2/57 (MCA469GA) used as part of a panel of antibodies to demonstrate the phenotype of canine mesenchymal stem cells by flow cytometry together with mesenchymal markers CD44, CD90 and CD105. Negative results for lineage cell markers CD34, CD45 and STRO-1 are shown.
Image caption:
b. Representative histograms demonstrating positive and negative staining of marrow cMSCs from a single donor

From: Bearden RN, Huggins SS, Cummings KJ, Smith R, Gregory CA, Saunders WB.
In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
Stem Cell Res Ther. 2017 Oct 3;8(1):218.
Anti Human CD9 Antibody, clone MM2/57 gallery image 5
Published customer image:
Mouse anti Human CD9 antibody, clone MM2/57 (MCA649G) used for the evaluation of CD9 expression in canine cell line lysates by western blotting.
Image caption:
Insert image caption

From: Fish EJ, Irizarry KJ, DeInnocentes P, Ellis CJ, Prasad N, Moss AG, Curt Bird R.
Malignant canine mammary epithelial cells shed exosomes containing differentially expressed microRNA that regulate oncogenic networks.
BMC Cancer. 2018 Aug 20;18(1):832.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD9 Antibody, clone MM2/57 gallery image 6
Published customer image:
Mouse anti Human CD9 antibody, clone MM2/57 (MCA469G) used for the evaluation of CD9 expression in lysates from human melanoma derived extracellular vesicles by immunoblotting.
Image caption:
Localization of palmNG in EVs. Various amounts of EVs from MJS or MJSpalmNG cells were dot-blotted in a dose range on a nitrocellulose membrane and analyzed by immunoblotting with the use of anti-mNeonGreen or anti-CD9 antibody in the presence or absence of a detergent.

From: Wąchalska, M. et al.
Palmitoylated mNeonGreen Protein as a Tool for Visualization and Uptake Studies of Extracellular Vesicles.
Membranes 2020, 1(12),373.
10.3390/membranes10120373..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human CD9 Antibody, clone MM2/57 gallery image 7
Published customer image:
Mouse anti Human CD9 antibody, clone MM2/57 (MCA469G) used to evaluate exosome levels in virally infected MDCK cell lysates by western blotting.
Image caption:
Identification and characterization of exosomes.(c) Immunoblotting of exosomal marker (CD9) and IAV matrix protein (M1). Exosomes secreted by mock–infected (mock 1, 2) and IAV infected (flu 1, 2) cells; IAV–influenza virions; MDCK–mock–infected (−) and IAV infected cells (+).

From: Kwasnik M, Socha W, Czech B, Wasiak M, Rola J, Rozek W.
Protein-Coding Region Derived Small RNA in Exosomes from Influenza A Virus-Infected Cells.
Int J Mol Sci. 2023 Jan 3;24(1):867.
doi: 10.3390/ijms24010867.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human CD9 Antibody, clone MM2/57 gallery image 8
Published customer image:
Mouse anti Human CD9 antibody, clone MM2/57 (MCA469G) used to label extracellular vesicles (EVs) from MDBK and MJS cells by western blotting.
Image caption:
Alphaherpesvirus gB localizes to EVs-enriched fractions isolated from virus-infected MJS cells and BoHV-1-infected MDBK. OptiPrep gradient-purification of EVs-enriched and virion-enriched fractions from HSV-1-infected MJS (A), BoHV-1-infected MJS (B), PRV-infected MJS (C), and BoHV-1-infected MDBK (D) supernatants. MJS cells were infected with HSV-1 at a multiplicity of infection (moi) of 0.1, with BoHV-1/PRV at an moi of 0.5; MDBK cells were infected at an moi of 0.1. After 50 h post-infection, 0.5 mL fractions were collected from 6%–18% iodixanol (Optiprep) gradient by ultracentrifugation. The 40 μL samples were analyzed in denaturing (for viral glycoprotein C-gC, glycoprotein B-gB, glycoprotein D-gD, for HSV-1, glycoprotein E-gE, for BoHV-1 and PRV or EVs marker Alix) or non-denaturing (for EVs markers CD63 and CD9) SDS-PAGE, and immunoblotted with specific mAbs or polyclonal anti-PRV gB serum. Uninfected (ø) or virus-infected cell lysates (cl) were analyzed as controls. Bottom panels represent virus titres in each fraction measured by plaque assay.

From: Grabowska K, Wąchalska M, Graul M, Rychłowski M, Bieńkowska-Szewczyk K, Lipińska AD.
Alphaherpesvirus gB Homologs Are Targeted to Extracellular Vesicles, but They Differentially Affect MHC Class II Molecules.
Viruses. 2020 Apr 10;12(4):429.
doi: 10.3390/v12040429.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human CD9 Antibody, clone MM2/57 gallery image 9
Published customer image:
Insert meta-description and applications
Image caption:
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From: Milburn JV, Hoog AM, Winkler S, van Dongen KA, Leitner J, Patzl M, Saalmüller A, de Luca K, Steinberger P, Mair KH, Gerner W.
Expression of CD9 on porcine lymphocytes and its relation to T cell differentiation and cytokine production.
Dev Comp Immunol. 2021 Aug;121:104080.
doi: 10.1016/j.dci.2021.104080.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Human CD9:Alexa Fluor® 488

Product Type
Monoclonal Antibody
Clone
MM2/57
Isotype
IgG2b
Specificity
CD9

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Product Code Applications Pack Size List Price Your Price Qty
MCA469A488
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
loader

Mouse anti Human CD9 antibody, clone MM2/57 recognizes human leukocyte antigen MIC3 also known as MRP-1 or CD9. CD9 is a 228 amino acid multi pass membrane glycoprotein belonging to the tetraspanin family with a molecular weight of ~24 kDa expressed by platelets, monocytes, some lymphocytes and endothelial cells.

Mouse anti Human CD9 antibody, clone MM2/57 recognizes a conserved epitope on CD9 present on a wide range of mammalian species.

Clone MM2/57 has shown weak cross reactivity to porcine CD9 (Milburn, et al. 2021).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cat
Rhesus Monkey
Bovine
Dog
Rabbit
Horse
Pig
Mustelid Expected from Sequence
Mink
Llama
Ferret
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Human platelet membranes
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells from the SP2/0 mouse myeloma line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®488 495 519
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2b Negative Control:Alexa Fluor® 488 MCA691A488 F 100 Tests/1ml loader
List Price Your Price
loader
Description Mouse IgG2b Negative Control:Alexa Fluor® 488

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD9 antibody

  1. Ed Knapp W. et al. (1989) Leucocyte Typing IV
    Oxford University Press.
  2. Jennings, L. K. et al. (1995) CD9 cluster workshop report: cell surface binding and functional analysis.
    In S.F. Sclossman. et al. Editors. 1995. Leucocyte Typing V. White Cell Differentiation Antigens. Oxford University Press, New York, NY. 1249-1251.
  3. Löffler, S. et al. (1997) CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus.
    J Virol. 71: 42-9.
  4. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
    Vet Immunol Immunopathol. 64 (1): 1-13.
  5. Ferrer, M. et al. (1998) Pattern of expression of tetraspanin antigen genes in Burkitt lymphoma cell lines.
    Clin Exp Immunol. 113: 346-52.
  6. Kao, Y.R. et al. (2003) Tumor-associated antigen L6 and the invasion of human lung cancer cells.
    Clin Cancer Res. 9: 2807-16.
  7. Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
    Vet Immunol Immunopathol. 119: 27-37.
  8. Davis, W.C. et al. (2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.
    Vet Immunol Immunopathol. 119: 123-30.
    9. View The Latest Product References
  9. Meister, R.K. et al. (2007) Progress in the discovery and definition of monoclonal antibodies for use in feline research.
    Vet Immunol Immunopathol. 119: 38-46.
  10. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
    Vet Immunol Immunopathol. 132:109-15.
  11. Müller, T. et al. (2009) A novel embryonic stem cell line derived from the common marmoset monkey (Callithrix jacchus) exhibiting germ cell-like characteristics.
    Hum Reprod. 24: 1359-72.
  12. Kubota, H. et al. (2011) Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
    FASEB J. 25 (8): 2604-14.
  13. Hogue, I.B. et al. (2011) Gag induces the coalescence of clustered lipid rafts and tetraspanin-enriched microdomains at HIV-1 assembly sites on the plasma membrane.
    J Virol. 85 (19): 9749-66.
  14. Viswanathan, K. et al. (2017) Quantitative membrane proteomics reveals a role for tetraspanin enriched microdomains during entry of human cytomegalovirus.
    PLoS One. 12 (11): e0187899.
  15. Bearden, R.N. et al. (2017) In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
    Stem Cell Res Ther. 8 (1): 218.
  16. Jackson, C.E. et al. (2017) Effects of Inhibiting VPS4 Support a General Role for ESCRTs in Extracellular Vesicle Biogenesis.
    Biophys J. 113 (6): 1342-1352.
  17. Fish, E.J. et al. (2018) Malignant canine mammary epithelial cells shed exosomes containing differentially expressed microRNA that regulate oncogenic networks.
    BMC Cancer. 18 (1): 832.
  18. Wąchalska, M. et al. (2020) Palmitoylated mNeonGreen Protein as a Tool for Visualization and Uptake Studies of Extracellular Vesicles.
    Membranes (Basel). 10 (12): 373.
  19. Fu, T.S. et al. (2023) Biomimetic vascularized adipose-derived mesenchymal stem cells bone-periosteum graft enhances angiogenesis and osteogenesis in a male rabbit spine fusion model.
    Bone Joint Res. 12 (12): 722-33.
  20. Kwasnik, M. et al. (2023) Protein-Coding Region Derived Small RNA in Exosomes from Influenza A Virus-Infected Cells.
    Int J Mol Sci. 24 (1): 867.
  21. Grabowska, K. et al. (2020) Alphaherpesvirus gB Homologs Are Targeted to Extracellular Vesicles, but They Differentially Affect MHC Class II Molecules.
    Viruses. 12 (4): 429.
  22. Milburn, J.V. et al. (2021) Expression of CD9 on porcine lymphocytes and its relation to T cell differentiation and cytokine production.
    Dev Comp Immunol. 121: 104080.

Flow Cytometry

Synonyms
MRP-1
UniProt
P21926
Entrez Gene
CD9
GO Terms
GO:0005515 protein binding
GO:0002576 platelet degranulation
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0006928 cellular component movement
GO:0007342 fusion of sperm to egg plasma membrane
GO:0030168 platelet activation
GO:0030913 paranodal junction assembly
GO:0031092 platelet alpha granule membrane

MCA469A488

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