CD87 antibody | VIM-5
Mouse anti Human CD87:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- VIM-5
- Isotype
- IgG1
- Specificity
- CD87
Mouse anti Human CD87 antibody, clone VIM-5 recognizes human CD87, also known as urokinase plasminogen activator receptor (uPAR), a 50-65 kDa single chain glycosylphostidylinsitol (GPI) anchored glycoprotein, expressed by granulocytes, tissue macrophages, monocytes, endothelial cells, fibroblasts and mesenchymal and epithelial tumour cells. CD87 specifically binds and activates urokinase plasminogen activator (uPA), a serine protease which converts the inactive receptor-bound proenzyme plasminogen into plasmin, triggering the proteolysis cascade of fibrinolysis. The interaction between uPAR and uPA is also known to play a role in cancer cell invasion and metastasis. Clone VIM-5 is reported to block urokinase binding. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin - Immunogen
- THP-1 cells (Human acute monocytic leukaemia cells).
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock |
References for CD87 antibody
-
Gadd, S.J. et al. (1990) M5, a phosphoinositol-linked human myelomonocytic activation-associated antigen.
Clin Exp Immunol. 80 (2): 252-6. -
Sillaber, C. et al. (1997) Molecular and functional characterization of the urokinase receptor on human mast cells.
J Biol Chem. 272 (12): 7824-32.
Further Reading
-
Mondino, A. & Blasi, F. (2004) uPA and uPAR in fibrinolysis, immunity and pathology.
Trends Immunol. 25 (8): 450-5.
MCA2516A647
153388MCA2516A647T
153389If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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