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CD85j antibody | 4F9

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Anti Human CD85j Antibody, clone 4F9 thumbnail image 1
Anti Human CD85j Antibody, clone 4F9 gallery image 1
Figure A. StarBright Blue 580 conjugated Mouse anti Human CD19 (MCA1940SBB580) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. StarBright Blue 580 conjugated Mouse anti Human CD19 (MCA1940SBB580) and Alexa Fluor 647 conjugated Mouse anti Human CD85j (MCA2515A647). All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD85j:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
4F9
Isotype
IgG1
Specificity
CD85j

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Product Code Applications Pack Size List Price Your Price Qty
MCA2515A647
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SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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Mouse anti Human CD85j antibody, clone 4F9 recognizes CD85j, a member of the leukocyte immunoglobulin-like receptor (LIR) family.

CD85j is a receptor for MHC Class I molecules and ligand binding results in inhibitory signals and down-regulation of the immune response.

CD85j is expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells, T-cells and natural killer (NK) cells.

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Monocyte derived dendritic cells.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse X63-Ag8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
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Description Mouse IgG1 Negative Control:Alexa Fluor® 647

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

Further Reading

  1. Colonna, M. et al. (1999) A novel family of Ig-like receptors for HLA class I molecules that modulate function of lymphoid and myeloid cells.
    J Leukoc Biol. 66 (3): 375-81.
  2. Borges, L. et al. (1997) A family of human lymphoid and myeloid Ig-like receptors, some of which bind to MHC class I molecules.
    J Immunol. 159 (11): 5192-6.

Synonyms
LILRB1
RRID
AB_915232
UniProt
Q8NHL6
Entrez Gene
LILRB1
GO Terms
GO:0005886 plasma membrane
GO:0009615 response to virus
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0008157 protein phosphatase 1 binding
GO:0050776 regulation of immune response
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