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CD84 antibody | CD84.1.21

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Anti Human CD84 Antibody, clone CD84.1.21 thumbnail image 1
Anti Human CD84 Antibody, clone CD84.1.21 gallery image 1
Figure A. Alexa Fluor 700 conjugated mouse anti human CD14 (MCA596A700) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. Alexa Fluor 700 conjugated mouse anti human CD14 (MCA596A700) and RPE conjugated mouse anti human CD84 (MCA2123PE). All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD84:FITC

Product Type
Monoclonal Antibody
Clone
CD84.1.21
Isotype
IgG1
Specificity
CD84

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Product Code Applications Pack Size List Price Your Price Qty
MCA2123F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Human CD84 antibody, clone CD84.1.21 recognizes human SLAM family member 5, also known as CD84, Cell surface antigen MAX.3, Hly9-beta or Signaling lymphocytic activation molecule 5. CD84 is a highly glycosylated cell surface antigen of ~64-82 kDa, predominantly expressed by B lymphocytes, monocytes and macrophages, although lower levels of expression have also been reported on T cells, granulocytes and platelets.

Mouse anti Human CD84 antibody, clone CD84.1.21 has been shown to partially block CD84 homophilic binding (Moore et al. 2001).

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Mouse pre-B cell line 300.19 transfected with CD84 cDNA.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
>
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

Source Reference

  1. Martin, M. et al. (2001) CD84 functions as a homophilic adhesion molecule and enhances IFN-gamma secretion: adhesion is mediated by Ig-like domain 1.
    J Immunol. 167 (7): 3668-76.

References for CD84 antibody

  1. Sayós J et al. (2001) Cell surface receptors Ly-9 and CD84 recruit the X-linked lymphoproliferative disease gene product SAP.
    Blood. 97 (12): 3867-74.
  2. Romero, X. et al. (2005) CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse.
    J Immunol. 174 (11): 7033-42.
  3. Mihalache, C.C. et al. (2011) Inflammation-associated autophagy-related programmed necrotic death of human neutrophils characterized by organelle fusion events.
    J Immunol. 186: 6532-42.
  4. Wang, X. et al. (2016) Neutrophil Necroptosis Is Triggered by Ligation of Adhesion Molecules following GM-CSF Priming.
    J Immunol. 197 (10): 4090-100.

RRID
AB_324182
UniProt
Q9UIB8
Entrez Gene
CD84

MCA2123F

148188

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