CD8 antibody | YTC182.20
Rat anti Human CD8:FITC
- Product Type
- Monoclonal Antibody
- Clone
- YTC182.20
- Isotype
- IgG2b
- Specificity
- CD8
Rat anti Human CD8 antibody, clone YTC182.20 recognizes the human CD8 cell surface antigen, a ~30/32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Mouse L cells transfected with human CD8 gene.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag.1.2.3 myeloma line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Rat IgG2b Negative Control:FITC | MCA6006F | F | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Rat IgG2b Negative Control:FITC |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
References for CD8 antibody
-
Jonker, M. et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocytes.
Leucocyte Typing IV. Oxford University Press p 1058-1063. -
Walton, L.J. et al. (1998) Intra-epithelial subpopulations of T lymphocytes and Langerhans cells in oral lichen planus.
J Oral Pathol Med. 27 (3): 116-23. -
Shacklett, B.L. et al. (2004) Abundant expression of granzyme A, but not perforin, in granules of CD8+ T cells in GALT: implications for immune control of HIV-1 infection.
J Immunol. 173 (1): 641-8. -
Salajegheh, M. et al. (2007) Upregulation of thrombospondin-1(TSP-1) and its binding partners, CD36 and CD47, in sporadic inclusion body myositis.
J Neuroimmunol. 187 (1-2): 166-74. -
Salajegheh, M. et al. (2007) T cell receptor profiling in muscle and blood lymphocytes in sporadic inclusion body myositis.
Neurology. 69 (17): 1672-9. -
Sasikala-Appukuttan, A.K. et al. (2013) Location and dynamics of the immunodominant CD8 T cell response to SIVΔnef immunization and SIVmac251 vaginal challenge.
PLoS One. 8 (12): e81623. -
Willing, A. et al. (2014) CD8⁺ MAIT cells infiltrate into the CNS and alterations in their blood frequencies correlate with IL-18 serum levels in multiple sclerosis.
Eur J Immunol. 44 (10): 3119-28. -
Wang, H. et al. (2016) Assessment of placental transfer and the effect on embryo-fetal development of a humanized monoclonal antibody targeting lymphotoxin-alpha in non-human primates.
Reprod Toxicol. 63: 82-95.
View The Latest Product References
-
Cramer, M.L. et al. (2017) Induction of T-Cell Infiltration and Programmed Death Ligand 2 Expression by Adeno-Associated Virus in Rhesus Macaque Skeletal Muscle and Modulation by Prednisone.
Hum Gene Ther. 28 (6): 493-509. -
van, G.N. et al. (2017) Phenotypic and functional characterization of T cells in white matter lesions of multiple sclerosis patients.
Acta Neuropathol. 134 (3): 383-401.
- RRID
- AB_321404
- UniProt
- P01732
- P10966
- Entrez Gene
- CD8A
- CD8B
- GO Terms
- GO:0005576 extracellular region
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0005887 integral to plasma membrane
- GO:0015026 coreceptor activity
- GO:0019882 antigen processing and presentation
- GO:0042101 T cell receptor complex
- GO:0042288 MHC class I protein binding
- GO:0050776 regulation of immune response
MCA351F
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