CD8 antibody | YTC182.20

Rat anti Human CD8:FITC

Product Type
Monoclonal Antibody
Clone
YTC182.20
Isotype
IgG2b
Specificity
CD8

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MCA351F
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Rat anti Human CD8 antibody, clone YTC182.20 recognizes the human CD8 cell surface antigen, a ~30/32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Mouse L cells transfected with human CD8 gene.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag.1.2.3 myeloma line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.

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References for CD8 antibody

  1. Jonker, M. et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocytes.
    Leucocyte Typing IV. Oxford University Press p 1058-1063.
  2. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49 (2): 97-110.
  3. Walton, L.J. et al. (1998) Intra-epithelial subpopulations of T lymphocytes and Langerhans cells in oral lichen planus.
    J Oral Pathol Med. 27 (3): 116-23.
  4. Shacklett, B.L. et al. (2004) Abundant expression of granzyme A, but not perforin, in granules of CD8+ T cells in GALT: implications for immune control of HIV-1 infection.
    J Immunol. 173 (1): 641-8.
  5. Wang, H. et al. (2016) Assessment of placental transfer and the effect on embryo-fetal development of a humanized monoclonal antibody targeting lymphotoxin-alpha in non-human primates.
    Reprod Toxicol. 63: 82-95.
  6. Salajegheh, M. et al. (2007) T cell receptor profiling in muscle and blood lymphocytes in sporadic inclusion body myositis.
    Neurology. 69 (17): 1672-9.
  7. Salajegheh, M. et al. (2007) Upregulation of thrombospondin-1(TSP-1) and its binding partners, CD36 and CD47, in sporadic inclusion body myositis.
    J Neuroimmunol. 187 (1-2): 166-74.
  8. Sasikala-Appukuttan, A.K. et al. (2013) Location and dynamics of the immunodominant CD8 T cell response to SIVΔnef immunization and SIVmac251 vaginal challenge.
    PLoS One. 8 (12): e81623.
  9. View The Latest Product References
  10. VanNierop, G.P. et al. (2017) Phenotypic and functional characterization of T cells in white matter lesions of multiple sclerosis patients.
    Acta Neuropathol. Jun 17 [Epub ahead of print].
  11. Cramer, M.L. et al. (2017) Induction of T-Cell Infiltration and Programmed Death Ligand 2 Expression by Adeno-Associated Virus in Rhesus Macaque Skeletal Muscle and Modulation by Prednisone.
    Hum Gene Ther. 28 (6): 493-509.
  12. Willing, A. et al. (2014) CD8⁺ MAIT cells infiltrate into the CNS and alterations in their blood frequencies correlate with IL-18 serum levels in multiple sclerosis.
    Eur J Immunol. 44 (10): 3119-28.

RRID
AB_321404
UniProt
P01732
P10966
Entrez Gene
CD8A
CD8B
GO Terms
GO:0005576 extracellular region
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0015026 coreceptor activity
GO:0019882 antigen processing and presentation
GO:0042101 T cell receptor complex
GO:0042288 MHC class I protein binding
GO:0050776 regulation of immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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