CD69 antibody | FN50
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|Mouse anti Human CD69 antibody, clone FN50 recognizes the human early activation antigen CD69, also known as activation inducer molecule (AIM), Early T-cell activation antigen p60, EA1 or MLR-3. CD69 is a 199 amino acid single pass type II transmembrane glycoprotein of ~30 kDa containing a single C-type lectin domain and a single potential N-glycosylation site. CD69 is expressed as a disulphide bond linked homodimer of ~60 kDa (López-Cabrera et al. 1993).
CD69 is a marker of early activation expressed by B and T lymphocytes, natural killer cells(Werfel
Mouse anti Human CD69 , clone FN50 is useful for the detection of CD69 by flow cytometry and immunohistochemistry on frozen tissue sections.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Baboon Chimpanzee Cynomolgus monkey Rhesus Monkey Macaque
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to StarBright Violet 760 - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
- Activated human B-cells.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 760 403 754
- For research purposes only
- 12 months from date of despatch
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
This product should be stored undiluted.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
References for CD69 antibody
Holte, H. et al. (1989) Ki67 and 4F2 antigen expression as well as DNA synthesis predict survival at relapse/tumour progression in low-grade B-cell lymphoma.
Int J Cancer. 44 (6): 975-80.
Herberth, M. et al. (2010) Differential effects on T-cell function following exposure to serum from schizophrenia smokers.
Mol Psychiatry. 15 (4): 364-71.
Schaeuble, K. et al. (2011) Cross-talk between TCR and CCR7 signaling sets a temporal threshold for enhanced T lymphocyte migration.
J Immunol. 187 (11): 5645-52.
Sela, M. et al. (2011) Sequential phosphorylation of SLP-76 at tyrosine 173 is required for activation of T and mast cells.
EMBO J. 30 (15): 3160-72.
Garbe, Y. et al. (2011) Semiallogenic fusions of MSI(+) tumor cells and activated B cells induce MSI-specific T cell responses.
BMC Cancer. 11: 410.
Schwitalle, Y. et al. (2004) Immunogenic peptides generated by frameshift mutations in DNA mismatch repair-deficient cancer cells.
Cancer Immun. 4: 14.
Sutavani, R.V. et al. (2013) CD55 Costimulation Induces Differentiation of a Discrete T Regulatory Type 1 Cell Population with a Stable Phenotype.
J Immunol. 191: 5895-903.
Walter, G.J. et al. (2013) Interaction with activated monocytes enhances cytokine expression and suppressive activity of human CD4+CD45ro+CD25+CD127(low) regulatory T cells.
Arthritis Rheum. 65: 627-38.
View The Latest Product References
Kuric, E. et al. (2017) Demonstration of Tissue Resident Memory CD8 T Cells in Insulitic Lesions in Adult Patients with Recent-Onset Type 1 Diabetes.
Am J Pathol. 187 (3): 581-8.
Karnell, F.G. et al. (2017) Reconstitution of immune cell populations in multiple sclerosis patients after autologous stem cell transplantation.
Clin Exp Immunol. 189 (3): 268-278.
Rossatti, P. et al. (2022) Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation.
BMC Biol. 20 (1): 189.
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