CD62P antibody | Psel.KO.2.5
Mouse anti Human CD62P:StarBright Violet 515
- Product Type
- Monoclonal Antibody
- Clone
- Psel.KO.2.5
- Isotype
- IgG1
- Specificity
- CD62P
Mouse anti Human CD62P antibody, clone Psel.KO.2.5 recognizes the CD62P cell surface antigen, a ~140 kDa glycoprotein, also known as P-selectin. CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to StarBright Violet 515 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Immunogen
- CD62P transfected 300.19 cells.
- Fusion Partners
- Spleen cells from immunised CD62P knock-out mice (strain C57/B6) were fused with cells of the NS-1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 515 402 516 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
This product should be stored undiluted.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
Source Reference
-
Massaguer, A. et al. (2000) Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P).
Tissue Antigens. 56 (2): 117-28.
References for CD62P antibody
-
Massaguer, A. et al. (2003) Characterization of platelet and soluble-porcine P-selectin (CD62P).
Vet Immunol Immunopathol. 96 (3-4): 169-81. -
Johnson, C.A. Jr. et al. (2011) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.
Artif Organs. 35 (6): 602-13. -
Krajewski, S. et al. (2012) Flow cytometry analysis of porcine platelets: optimized methods for best results.
Platelets. 23: 386-94. -
Shankarraman, V. et al. (2014) Biocompatibility Assessment of the CentriMag-Novalung Adult ECMO Circuit in a Model of Acute Pulmonary Hypertension.
ASAIO J. 60 (4): 429-35. -
Tunjungputri, R.N. et al. (2016) Invasive pneumococcal disease leads to activation and hyperreactivity of platelets.
Thromb Res. 144: 123-6. -
Chan, C.H.H. et al. (2017) Shear Stress-Induced Total Blood Trauma in Multiple Species.
Artif Organs. 41 (10): 934-47. -
Batchinsky, A.I. et al. (2023) Intravenous Autologous Bone-Marrow-derived Mesenchymal Stromal Cells Delay Acute Respiratory Distress Syndrome in Swine.
Am J Respir Crit Care Med. Oct 05 [Epub ahead of print].
Further Reading
-
Piriou-Guzylack, L. & Salmon, H. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39 (6): 54.
- Synonyms
- P-SELECTIN
- UniProt
- P16109
- Entrez Gene
- SELP
- GO Terms
- GO:0001530 lipopolysaccharide binding
- GO:0002576 platelet degranulation
- GO:0005887 integral to plasma membrane
- GO:0005615 extracellular space
- GO:0005624 membrane fraction
- GO:0005625 soluble fraction
- GO:0008201 heparin binding
- GO:0050829 defense response to Gram-negative bacterium
- GO:0009897 external side of plasma membrane
- View More GO Terms
- GO:0010572 positive regulation of platelet activation
- GO:0030168 platelet activation
- GO:0031088 platelet dense granule membrane
- GO:0031092 platelet alpha granule membrane
- GO:0032496 response to lipopolysaccharide
- GO:0033691 sialic acid binding
- GO:0042806 fucose binding
- GO:0043208 glycosphingolipid binding
- GO:0070492 oligosaccharide binding
MCA2418SBV515
100008214If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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