CD62P antibody | AK-6
Mouse anti Human CD62P:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
- Clone
- AK-6
- Isotype
- IgG1
- Specificity
- CD62P
Mouse anti Human CD62P antibody, clone AK-6 recognizes the CD62P, also known as P-selectin, Granule membrane protein 140, GMP140, Leukocyte-endothelial cell adhesion molecule 3 or Platelet activation dependent granule-external membrane protein. CD62P is a 830 amino acid, including a 41 amino acid signal peptide, ~140 kDa single pass type I transmembrane glycoprotein expressed on activated platelets and endothelial cell CD62P plays an important role in adhesive processes between leucocytes and endothelial cells. CD62P is a component of the platelet alpha granule and is rapidly translocated to the plasma membrane upon activation (Stenberg et al. 1985). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Human platelet membrane glycoproteins.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG1 Negative Control:Alexa Fluor® 488 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
References for CD62P antibody
-
Skinner, M.P. et al. (1989) Characterization of human platelet GMP-140 as a heparin-binding protein.
Biochem Biophys Res Commun. 164 (3): 1373-9. -
Skinner, M.P. et al. (1991) GMP-140 binding to neutrophils is inhibited by sulfated glycans.
J Biol Chem. 266 (9): 5371-4. -
Dunlop, L.C. et al. (1992) Characterization of GMP-140 (P-selectin) as a circulating plasma protein.
J Exp Med. 175 (4): 1147-50. -
Theoret, J.F. et al. (2001) P-selectin antagonism with recombinant p-selectin glycoprotein ligand-1 (rPSGL-Ig) inhibits circulating activated platelet binding to neutrophils induced by damaged arterial surfaces.
J Pharmacol Exp Ther. 298: 658-64 -
Turner, C.P. et al. (2003) The role of P-selectin in the immune destruction of platelets.
Br J Haematol. 121: 623-31. -
Roos-Engstrand, E. et al. (2005) Increased expression of p38 MAPK in human bronchial epithelium after lipopolysaccharide exposure.
Eur Respir J. 25 (5): 797-803. -
Norling, L.V. et al. (2008) Inhibitory control of endothelial galectin-1 on in vitro and in vivo lymphocyte trafficking.
FASEB J. 22: 682-90. -
Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
Blood. 112 (6): 2512-9.
View The Latest Product References
-
Wassmer, S.C. at al. (2008) Platelet-induced clumping of Plasmodium falciparum-infected erythrocytes from Malawian patients with cerebral malaria-possible modulation in vivo by thrombocytopenia.
J Infect Dis. 197: 72-8. -
Kornerup, K.N. et al. (2010) Circulating platelet-neutrophil complexes are important for subsequent neutrophil activation and migration.
J Appl Physiol. 109: 758-67. -
van Nispen tot Pannerden, H. et al. (2010) The platelet interior revisited: electron tomography reveals tubular alpha-granule subtypes.
Blood. 116: 1147-56. -
Knipe, L. et al. (2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells.
Blood. 116 (12): 2183-91. -
Kitaya, K. & Yasuo, T. (2010) Aberrant expression of selectin E, CXCL1, and CXCL13 in chronic endometritis.
Mod Pathol. 23 (8): 1136-46. -
Christersson, C. et al. (2013) Evaluation of microparticles in whole blood by multicolour flow cytometry assay.
Scand J Clin Lab Invest. 73(3): 229-39. -
Xiong, G.M. et al. (2015) Imparting electroactivity to polycaprolactone fibers with heparin-doped polypyrrole: Modulation of hemocompatibility and inflammatory responses.
Acta Biomater. 23: 240-9. -
Liao, Y. et al. (2017) Tailoring of TiO2 films by H2SO4 treatment and UV irradiation to improve anticoagulant ability and endothelial cell compatibility.
Colloids Surf B Biointerfaces. 155: 314-22. -
Cipok, M. et al. (2019) Pathogenic heparin-induced thrombocytopenia and thrombosis (HIT) antibodies determined by rapid functional flow cytometry.
Eur J Haematol. 103 (3): 225-233. -
Jiang, T. et al. (2019) Hyaluronic Acid Nanoparticle Composite Films Confer Favorable Time-Dependent Biofunctions for Vascular Wound Healing.
ACS Biomater Sci Eng. 5 (4): 1833-48. -
Tardy-Poncet, B. et al. (2021) Functional Flow Cytometric Assay for Reliable and Convenient Heparin-Induced Thrombocytopenia Diagnosis in Daily Practice
Biomedicines. 9 (4): 332. -
Khandagale, A. et al. (2020) Role of Extracellular Vesicles in Pulmonary Arterial Hypertension: Modulation of Pulmonary Endothelial Function and Angiogenesis.
Arterioscler Thromb Vasc Biol. 40 (9): 2293-309. -
Åberg, M. et al. (2022) Platelet-leukocyte aggregate formation and inflammation in patients with pulmonary arterial hypertension and CTEPH.
Platelets. 33 (8): 1199-207.
Further Reading
-
Bevilacqua, M.P. & Nelson, R.M. (1993) Selectins.
J Clin Invest. 91 (2): 379-87.
- Synonyms
- P-SELECTIN
- RRID
- AB_1125277
- UniProt
- P16109
- Entrez Gene
- SELP
- GO Terms
- GO:0001530 lipopolysaccharide binding
- GO:0002576 platelet degranulation
- GO:0005887 integral to plasma membrane
- GO:0005615 extracellular space
- GO:0005624 membrane fraction
- GO:0005625 soluble fraction
- GO:0008201 heparin binding
- GO:0050829 defense response to Gram-negative bacterium
- GO:0009897 external side of plasma membrane
- View More GO Terms
- GO:0010572 positive regulation of platelet activation
- GO:0030168 platelet activation
- GO:0031088 platelet dense granule membrane
- GO:0031092 platelet alpha granule membrane
- GO:0032496 response to lipopolysaccharide
- GO:0033691 sialic acid binding
- GO:0042806 fucose binding
- GO:0043208 glycosphingolipid binding
- GO:0070492 oligosaccharide binding
MCA796A488
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