CD62L antibody | FMC46

Mouse anti Human CD62L:StarBright Violet 515

Product Type
Monoclonal Antibody
Clone
FMC46
Isotype
IgG2b
Specificity
CD62L

Product Code Applications Pack Size List Price Your Price Qty
MCA1076SBV515
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Mouse anti Human CD62L antibody, clone FMC46 recognizes human CD62L, also known a L-selectin, a 74-95 kDa member of the selectin family of adhesion receptors, which acts as a ligand for both CD62P (P-selectin) and CD62E (E-selectin). Human CD62L is constitutively expressed on most leucocytes including monocytes, granulocytes, lymphocytes, NK cells, bone marrow myeloid progenitor cells and on a subset of thymocytes.

CD62L plays an important role in leucocyte tethering and rolling on the endothelial cell surface and for the homing of naïve lymphocytes to lymph nodes and Peyers patches via HEV. Neutrophils require a constant supply of this molecule on the cell surface for migration into peripheral tissues and adhesion to activated endothelium at sites of inflammation, where CD62L is rapidly shed as soluble L-selectin, but surface expression still remains.

The expression of CD62L is down regulated on lymphocytes and neutrophils by PMA stimulation.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Cynomolgus monkey
Rhesus Monkey
Dog
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to StarBright Violet 515 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
PHA stimulated lymphoblasts
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 515 401 516
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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References for CD62L antibody

  1. Pilarski, L.M. et al. (1991) FMC46, a cell protrusion-associated leukocyte adhesion molecule-1 epitope on human lymphocytes and thymocytes.
    J Immunol. 147 (1): 136-43.
  2. Zola, H. et al. (1991) The expression of sub-population markers on B cells: a re-evaluation using high-sensitivity fluorescence flow cytometry.
    Dis Markers. 9 (2): 103-18.
  3. Sopp, P. & Howard, C.J. (1997) Cross-reactivity of monoclonal antibodies to defined human leucocyte differentiation antigens with bovine cells.
    Vet Immunol Immunopathol. 56 (1-2): 11-25.
  4. Haanstra, K.G. et al. (2008) Characterization of naturally occurring CD4+CD25+ regulatory T cells in rhesus monkeys.
    Transplantation 85:1185-92.
  5. Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
    Blood. 112 (6): 2512-9.
  6. Raposo, R.A. et al. (2011) Protein Kinase C and NF-{kappa}B-Dependent CD4 Downregulation in Macrophages Induced by T Cell-Derived Soluble Factors: Consequences for HIV-1 Infection.
    J Immunol. 187: 748-59.
  7. Hughes, S.F. et al. (2010) Total hip and knee replacement surgery results in changes in leukocyte and endothelial markers.
    J Inflamm (Lond). 7:2.
  8. Bismarck, D. et al. (2012) Canine CD4+CD8+ double positive T cells in peripheral blood have features of activated T cells.
    Vet Immunol Immunopathol. 149: 157-66.
  9. View The Latest Product References
  10. Hartley, A.N. & Tarleton, R.L. (2015) Chemokine receptor 7 (CCR7)-expression and IFNγ production define vaccine-specific canine T-cell subsets.
    Vet Immunol Immunopathol. 164 (3-4): 127-36.
  11. Hayhoe, R.P. et al. (2006) Annexin 1 and its bioactive peptide inhibit neutrophil-endothelium interactions under flow: indication of distinct receptor involvement.
    Blood. 107 (5): 2123-30.
  12. Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
    J Pharmacol Exp Ther. 321 (2): 656-62.
  13. Aspinall, A.I. et al. (2010) CX(3)CR1 and vascular adhesion protein-1-dependent recruitment of CD16(+) monocytes across human liver sinusoidal endothelium.
    Hepatology. 51 (6): 2030-9.
  14. Rothe, K. et al. (2017) Canine peripheral blood CD4+CD8+ double-positive Tcell subpopulations exhibit distinct Tcell phenotypes and effector functions.
    Vet Immunol Immunopathol. 185: 48-56.
  15. Withers, S.S. et al. (2018) Multi-color flow cytometry for evaluating age-related changes in memory lymphocyte subsets in dogs.
    Dev Comp Immunol. 87: 64-74.
  16. Hughes, S.F. et al. (2020) The role of phagocytic leukocytes following flexible ureterenoscopy, for the treatment of kidney stones: an observational, clinical pilots-study.
    Eur J Med Res. 25 (1): 68.
  17. Svitek, N. et al. (2018) An Ad/MVA vectored Theileria parva antigen induces schizont-specific CD8+ central memory T cells and confers partial protection against a lethal challenge.
    NPJ Vaccines. 3: 35.

Flow Cytometry

Synonyms
LECAM-1
L-SELECTIN
UniProt
P14151
Entrez Gene
SELL
GO Terms
GO:0007596 blood coagulation
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
GO:0008201 heparin binding
GO:0043208 glycosphingolipid binding
GO:0050776 regulation of immune response
GO:0050900 leukocyte migration
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