CD6 antibody | MEM-98





Multiple gene transcripts have been detected encoding CD6 in man resulting in the production of a number of CD6 isoforms. Mouse anti human CD6, clone MEM-98 recognizes an external epitope located in the membrane-distal SRCR domain 1 and is expected to recognize all CD6 isoforms so far identified, CD6A-E.
CD6 has been implicated as a therapeutic target for a number of autoimmune conditions (Pinto et al. 2013) including Sjögren's syndrome (Ramos-Casals et al. 2001) , rheumatoid arthritis (Rodriguez et al.) and psoriasis (Wilsmann-Theis et al. 2006).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Purified CD6 antigen
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Fusion Partners
- Spleen cells from immunised mice.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P30203
- Entrez Gene
- CD6
- GO Terms
- GO:0007155 cell adhesion
- GO:0005044 scavenger receptor activity
- GO:0005887 integral to plasma membrane
- Regulatory
- For research purposes only
Applications of CD6 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD6 antibody
-
Bazil, V. et al. (1989) Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.
Folia Biol (Praha). 35 (5): 289-97. -
Nair, P. et al. (2010) CD6 synergistic co-stimulation promoting proinflammatory response is modulated without interfering with the activated leucocyte cell adhesion molecule interaction.
Clin Exp Immunol. 162 (1): 116-30. -
Castro, M.A. et al. (2007) Extracellular isoforms of CD6 generated by alternative splicing regulate targeting of CD6 to the immunological synapse.
J Immunol. 178 (7): 4351-61. -
Hassan, N.J. et al. (2006) CD6 regulates T-cell responses through activation-dependent recruitment of the positive regulator SLP-76.
Mol Cell Biol. 26 (17): 6727-38. -
Bughani, U. et al. (2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
PLoS One. 12 (7): e0180088. -
LI, G. et al. (2018) CD6 monoclonal antibodies differ in epitope, kinetics and mechanism of action.
Immunology. 155 (2): 273-82.
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