CD6 antibody | MEM-98

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Mouse anti Human CD6:FITC

Mouse anti Human CD6

Product Type
Monoclonal Antibody
Clone
MEM-98
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1880F F 0.1 mg
MCA1880GA F IP WB 0.1 mg
MCA1880 F IP WB 0.2 mg
Mouse anti Human CD6 antibody, clone MEM-98 recognizes human CD6, also known as T12 or TP120. CD6 is a ~100-130 kDa type 1 single pass trans-membrane protein member of the immunoglobulin superfamily. CD6 possesses 3 scavenger receptor cysteine rich (SRCR) domains in it’s extracellular sequence. The membrane proximal SRCR3 contains the epitope responsible for interaction with CD166, also known as Activated Leukocyte Cell Adhesion Molecule (ALCAM) or CD166.

Multiple gene transcripts have been detected encoding CD6 in man resulting in the production of a number of CD6 isoforms. Mouse anti human CD6, clone MEM-98 recognizes an external epitope located in the membrane-distal SRCR domain 1 and is expected to recognize all CD6 isoforms so far identified, CD6A-E.

CD6 has been implicated as a therapeutic target for a number of autoimmune conditions (Pinto et al. 2013) including Sjögren's syndrome (Ramos-Casals et al. 2001) , rheumatoid arthritis (Rodriguez et al.) and psoriasis (Wilsmann-Theis et al. 2006).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified CD6 antigen
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised mice.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P30203 Related reagents
Entrez Gene
CD6 Related reagents
GO Terms
GO:0007155 cell adhesion
GO:0005044 scavenger receptor activity
GO:0005887 integral to plasma membrane
Regulatory
For research purposes only

Applications of CD6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/25 Neat Pack Size: 0.1 mg
1/100 Pack Size: 0.2 mg
Immunoprecipitation
Western Blotting Non-reducing conditions
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Surface Plasmon Resonance

Product Specific References

References for CD6 antibody

  1. Bazil, V. et al. (1989) Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.
    Folia Biol (Praha). 35 (5): 289-97.
  2. Nair, P. et al. (2010) CD6 synergistic co-stimulation promoting proinflammatory response is modulated without interfering with the activated leucocyte cell adhesion molecule interaction.
    Clin Exp Immunol. 162 (1): 116-30.
  3. Castro, M.A. et al. (2007) Extracellular isoforms of CD6 generated by alternative splicing regulate targeting of CD6 to the immunological synapse.
    J Immunol. 178 (7): 4351-61.
  4. Hassan, N.J. et al. (2006) CD6 regulates T-cell responses through activation-dependent recruitment of the positive regulator SLP-76.
    Mol Cell Biol. 26 (17): 6727-38.
  5. Bughani, U. et al. (2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
    PLoS One. 12 (7): e0180088.
  6. Garner, L.I. et al. (2018) CD6 mAbs differ in epitope, kinetics and mechanism of action.
    Immunology. May 17 [Epub ahead of print].