CD6 antibody | MEM-98
FITC conjugated Mouse anti Human CD6 antibody, clone MEM-98 (MCA1880F) used for the evaluation of CD6 expression on human lymphocytes by flow cytometry.
Image caption:
CD6 receptor on lymphocytes is not internalized and is occupied with Itolizumab.
Human PBMCs were left unstimulated or stimulated with soluble anti CD3 0.5 ng/ml (OKT-3) in presence of Iso Ab or Itolizumab at 10 μg/mL for 3 days. Post stimulation, cells were harvested and stained with anti CD6 Ab, MEM-98 clone (A) and anti-human IgG, Fc specific (B). In panel A, since the CD6 receptor is occupied with Itolizumab, MEM-98 (commercially available anti CD6 D1) could not bind in Itolizumab treated groups and hence no signal is observed. The positive signal with anti-human IgG, in Itolizumab treated group in panel B suggests that Itolizumab is occupying CD6 receptor on lymphocyte surface. Data is representative of at least 3 independent experiments.
From: Bughani U, Saha A, Kuriakose A, Nair R, Sadashivarao RB, Venkataraman R, et al. (2017)
T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
PLoS ONE 12(7): e0180088.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD6 antibody, clone MEM-98 (MCA1880) used for the evaluation of CD6 expression CD6 immune precipitated samples by western blotting.
Image caption:
From: Bughani U, Saha A, Kuriakose A, Nair R, Sadashivarao RB, Venkataraman R, et al. (2017)
T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
PLoS ONE 12(7): e0180088.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD6 antibody, clone MEM-98 recognizes human CD6, also known as T12 or TP120. CD6 is a ~100-130 kDa type 1 single pass trans-membrane protein member of the immunoglobulin superfamily. CD6 possesses 3 scavenger receptor cysteine rich (SRCR) domains in it’s extracellular sequence. The membrane proximal SRCR3 contains the epitope responsible for interaction with CD166, also known as Activated Leukocyte Cell Adhesion Molecule (ALCAM) or CD166.
Multiple gene transcripts have been detected encoding CD6 in man resulting in the production of a number of CD6 isoforms. Mouse anti human CD6, clone MEM-98 recognizes an external epitope located in the membrane-distal SRCR domain 1 and is expected to recognize all CD6 isoforms so far identified, CD6A-E.
CD6 has been implicated as a therapeutic target for a number of autoimmune conditions (Pinto et al. 2013) including Sjögren's syndrome (Ramos-Casals et al. 2001) , rheumatoid arthritis (Rodriguez et al.) and psoriasis (Wilsmann-Theis et al. 2006).
Multiple gene transcripts have been detected encoding CD6 in man resulting in the production of a number of CD6 isoforms. Mouse anti human CD6, clone MEM-98 recognizes an external epitope located in the membrane-distal SRCR domain 1 and is expected to recognize all CD6 isoforms so far identified, CD6A-E.
CD6 has been implicated as a therapeutic target for a number of autoimmune conditions (Pinto et al. 2013) including Sjögren's syndrome (Ramos-Casals et al. 2001) , rheumatoid arthritis (Rodriguez et al.) and psoriasis (Wilsmann-Theis et al. 2006).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Purified CD6 antigen
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Fusion Partners
- Spleen cells from immunised mice.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P30203
- Entrez Gene
- CD6
- GO Terms
- GO:0007155 cell adhesion
- GO:0005044 scavenger receptor activity
- GO:0005887 integral to plasma membrane
- Regulatory
- For research purposes only
Applications of CD6 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
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Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD6 antibody
-
Bazil, V. et al. (1989) Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.
Folia Biol (Praha). 35 (5): 289-97. -
Nair, P. et al. (2010) CD6 synergistic co-stimulation promoting proinflammatory response is modulated without interfering with the activated leucocyte cell adhesion molecule interaction.
Clin Exp Immunol. 162 (1): 116-30. -
Castro, M.A. et al. (2007) Extracellular isoforms of CD6 generated by alternative splicing regulate targeting of CD6 to the immunological synapse.
J Immunol. 178 (7): 4351-61. -
Hassan, N.J. et al. (2006) CD6 regulates T-cell responses through activation-dependent recruitment of the positive regulator SLP-76.
Mol Cell Biol. 26 (17): 6727-38. -
Bughani, U. et al. (2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
PLoS One. 12 (7): e0180088. -
LI, G. et al. (2018) CD6 monoclonal antibodies differ in epitope, kinetics and mechanism of action.
Immunology. 155 (2): 273-82.
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