CD54 antibody | 15.2
Mouse anti Human CD54:Pacific Blue®
- Product Type
- Monoclonal Antibody
- Clone
- 15.2
- Isotype
- IgG1
- Specificity
- CD54
Mouse anti Human CD54 antibody, clone 15.2 recognizes the human CD54 cell surface antigen also known as intracellular Adhesion Molecule -1 (ICAM-1) or Major group rhinovirus receptor. CD54 is expressed by many cells following activation by inflammatory mediators. It is a 505 amino acid with an additional 27 amino acid signal peptide ~90 kDa single pass type I transmembrane glycoprotein bearing 5 Ig-like C2-type domains. Mouse anti Human CD54 antibody, clone 15.2 blocks CD54 function (Berendt et al. 1992). Mouse anti Human CD54 antibody, clone 15.2 binds to an epitope on the N-terminal Ig-like domain within a region designated the L43 loop (Chakravorty and Craig 2005). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Pacific Blue® - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Human monocytes
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0-Ag14 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Pacific Blue® 410 455 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Pacific Blue® | MCA928PB | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse IgG1 Negative Control:Pacific Blue® |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
References for CD54 antibody
-
Dransfield, I. et al. (1992) Interaction of leukocyte integrins with ligand is necessary but not sufficient for function.
J Cell Biol. 116:1527-35. -
Berendt, A. et al. (1992) The binding site on ICAM-1 for plasmodium falciparum-infected erythrocytes overlaps, but is distinct from, the LFA-1- binding site.
Cell. 68: 71-81. -
Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
J Pharmacol Exp Ther. 321 (2): 656-62. -
Baratin, M. et al. (2007) Dissection of the role of PfEMP1 and ICAM-1 in the sensing of Plasmodium-falciparum-infected erythrocytes by natural killer cells.
PLos One 2: e228. -
van Buul, J.D. et al. (2010) Inside-out regulation of ICAM-1 dynamics in TNF-alpha-activated endothelium.
PLoS One 5: e11336. -
Diaz-Romero, J. et al. (2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.
J Cell Physiol. 214: 75-83. -
Di Lorenzo, A. et al. (2011) Endothelial reticulon-4B (Nogo-B) regulates ICAM-1-mediated leukocyte transmigration and acute inflammation.
Blood. 117: 2284-95. -
Porter, J.C. and Hall, A. (2009) Epithelial ICAM-1 and ICAM-2 regulate the egression of human T cells across the bronchial epithelium.
FASEB J. 23: 492-502.
View The Latest Product References
-
Corvaisier, M. et al. (2005) V gamma 9V delta 2 T cell response to colon carcinoma cells.
J Immunol. 175: 5481-8. -
Horrocks, P. et al. (2005) PfEMP1 expression is reduced on the surface of knobless Plasmodium falciparum infected erythrocytes.
J Cell Sci. 118: 2507-18. -
Lozanoska-Ochser, B. et al. (2008) Expression of CD86 on human islet endothelial cells facilitates T cell adhesion and migration.
J Immunol. 181: 6109-16. -
Norling, L.V. et al. (2008) Inhibitory control of endothelial galectin-1 on in vitro and in vivo lymphocyte trafficking.
FASEB J. 22 (3): 682-90. -
Baumer, Y. et al. (2011) Telomerase-based immortalization modifies the angiogenic/inflammatory responses of human coronary artery endothelial cells.
Exp Biol Med (Maywood). 236: 692-700. -
Lask, A. et al. (2011) TCR-independent killing of B cell malignancies by anti-third-party CTLs: the critical role of MHC-CD8 engagement.
J Immunol. 187 (4): 2006-14. -
Sommaggio, R. et al. (2012) Multiple Receptors Trigger Human NK Cell-Mediated Cytotoxicity against Porcine Chondrocytes.
J Immunol. 188: 2075-83. -
Murphy, A.J. et al. (2013) Anti-inflammatory functions of apolipoprotein a-I and high-density lipoprotein are preserved in trimeric apolipoprotein a-I.
J Pharmacol Exp Ther. 344: 41-9. -
Sumagin R et al. (2014) Transmigrated neutrophils in the intestinal lumen engage ICAM-1 to regulate the epithelial barrier and neutrophil recruitment.
Mucosal Immunol. 7 (4): 905-15. -
Sugden SM et al. (2017) HIV-1 Vpu Downmodulates ICAM-1 Expression, Resulting in Decreased Killing of Infected CD4+ T Cells by NK Cells.
J Virol. 91 (8): pii: e02442-16. -
Lennartz, F. et al. (2015) Mapping the Binding Site of a Cross-Reactive Plasmodium falciparum PfEMP1 Monoclonal Antibody Inhibitory of ICAM-1 Binding.
J Immunol. 195 (7): 3273-83. -
Schoppmeyer, R. et al. (2022) The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner.
Cell Rep. 38 (3): 110243. -
Grönloh, M.L.B. et al. (2023) Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM-1.
EMBO Rep. 24 (1): e55483. -
Grönloh, M.L.B. et al. (2023) Intercellular adhesion molecule 2 regulates diapedesis hotspots by allowing neutrophil crawling against the direction of flow.
Vasc Biol. 5 (1): e230005.
- Synonyms
- ICAM-1
- UniProt
- P05362
- Entrez Gene
- ICAM1
- GO Terms
- GO:0007157 heterophilic cell-cell adhesion
- GO:0001910 regulation of leukocyte mediated cytotoxicity
- GO:0002291 T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
- GO:0002693 positive regulation of cellular extravasation
- GO:0004888 transmembrane receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005178 integrin binding
- GO:0005615 extracellular space
- GO:0007159 leukocyte cell-cell adhesion
- View More GO Terms
- GO:0022614 membrane to membrane docking
- GO:0046813 virion attachment, binding of host cell surface receptor
- GO:0050776 regulation of immune response
- GO:0050900 leukocyte migration
- GO:0051856 adhesion to symbiont
- GO:0060333 interferon-gamma-mediated signaling pathway
MCA1615PB
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