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CD48 antibody | MEM-102

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Anti Human CD48 Antibody, clone MEM-102 thumbnail image 1 Anti Human CD48 Antibody, clone MEM-102 thumbnail image 2 Anti Human CD48 Antibody, clone MEM-102 thumbnail image 3 Anti Human CD48 Antibody, clone MEM-102 thumbnail image 4 Anti Human CD48 Antibody, clone MEM-102 thumbnail image 5
Anti Human CD48 Antibody, clone MEM-102 gallery image 1
Staining of human peripheral blood lymphocytes with Mouse anti Human CD48:RPE (MCA1053PE).
Anti Human CD48 Antibody, clone MEM-102 gallery image 2
Staining of human peripheral blood lymphocytes with Mouse anti Human CD48:APC (MCA1053APC).
Anti Human CD48 Antibody, clone MEM-102 gallery image 3
Staining of human peripheral blood lymphocytes with Mouse anti Human CD48 (MCA1053G).
Anti Human CD48 Antibody, clone MEM-102 gallery image 4
Staining of human peripheral blood lymphocytes with Mouse anti Human CD48:FITC (MCA1053F).
Anti Human CD48 Antibody, clone MEM-102 gallery image 5
Published customer image:
Mouse anti Human CD48 antibody, clone MEM-102 (MCA1053G) used for the demonstration of CD48 expression on cultured PBMCs by immunofluorescence.
Image caption:
Distribution of CD244 in PBMCs of patients with HAM/TSP.
After the culture for 8 hours, PBMCs were stained with antibodies against perforin, CD244 and CD48, and visualized through microscope. Two representative 3D images were shown in A and B. The image shows DAPI (blue), perforin (green), CD244 (orange) and CD48 (purple). In addition to DAPI and perforin (left), CD244 (middle) and CD48 (right) are merged. The white arrows indicate CD244 clustering at the cell contact area.

From: Enose-Akahata Y, Matsuura E, Oh U, Jacobson S (2009)
High Expression of CD244 and SAP Regulated CD8+ T Cell Responses of Patients with HTLV-I Associated Neurologic Disease.
PLoS Pathog 5(12): e1000682.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD48:FITC

Product Type
Monoclonal Antibody
Clone
MEM-102
Isotype
IgG1
Specificity
CD48

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Product Code Applications Pack Size List Price Your Price Qty
MCA1053F
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F 0.1 mg loader
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Mouse anti Human CD48 antibody, clone MEM-102 recognizes human CD48, also known as B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2 or Signaling lymphocytic activation molecule 2. CD 48, as a mature molecule is a 193 amino acid, ~45 kDa cell membrane glycoprotein containing 2 Ig-like domains.

CD48 acts as a counter-receptor for CD2 (Kato et al. 1992) and is involved in immune regulation and tolerance (McArdel et al. 2016)

Mouse anti Human CD48 antibody, clone MEM-102 is useful for the visualisation of CD48 expressing cells by immunofluorescence (Enose-Akahata et al. 2009) and flow cytometry and may be used to differentiate haemapoietic tumors, sarcomas and melanomas.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
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Source Reference

  1. Bazil, V. et al. (1989) Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.
    Folia Biol (Praha). 35 (5): 289-97.

References for CD48 antibody

  1. Korínek, V. et al. (1991) The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1.
    Immunogenetics. 33 (2): 108-12.
  2. Cinek, T. & Horejsí, V. (1992) The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases.
    J Immunol. 149 (7): 2262-70.
  3. Kanuga, N. et al. (2002) Characterization of genetically modified human retinal pigment epithelial cells developed for in vitro and transplantation studies.
    Invest Ophthalmol Vis Sci. 43: 546-55.
  4. Staffler, G. et al. (2003) Selective inhibition of T cell activation via CD147 through novel modulation of lipid rafts.
    J Immunol. 171 (4): 1707-14.
  5. Fukushima, K. et al. (2005) Functional role played by the glycosylphosphatidylinositol anchor glycan of CD48 in interleukin-18-induced interferon-gamma production.
    J Biol Chem. 280: 18056-62.
  6. Enose-Akahata, Y. et al. (2009) High expression of CD244 and SAP regulated CD8 T cell responses of patients with HTLV-I associated neurologic disease.
    PLoS Pathog. 5: e1000682.
  7. Larochelle, A. et al. (2011) Human and rhesus macaque hematopoietic stem cells cannot be purified based only on SLAM family markers.
    Blood. 117:1550-4.
  8. Peterson, L.A. et al. (2019) Individual Differences in the Response of Human β-Lymphoblastoid Cells to the Cytotoxic, Mutagenic, and DNA-Damaging Effects of a DNA Methylating Agent, N-Methylnitrosourethane.
    Chem Res Toxicol. 32 (11): 2214-26.
    9. View The Latest Product References

Western Blotting

Synonyms
BLAST-1
RRID
AB_321437
UniProt
P09326
Entrez Gene
CD48
GO Terms
GO:0005515 protein binding
GO:0006952 defense response
GO:0007596 blood coagulation
GO:0005887 integral to plasma membrane
GO:0045121 membrane raft
GO:0050900 leukocyte migration

MCA1053F

150242 1608

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