CD45RO antibody | UCHL1
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F ResetMouse anti Human CD45RO:RPE-Cy5
- Product Type
- Monoclonal Antibody
- Clone
- UCHL1
- Isotype
- IgG2a
- Specificity
- CD45RO
Mouse anti Human CD45RO monoclonal antibody, clone UCHL1 recognizes the low molecular weight isoform (180 kDa) of the leucocyte common antigen (LCA). The antigen is expressed by a functional subset of T cells with memory phenotype. In peripheral blood Mouse anti Human CD45RO, clone UCHL1 stains 40-80% of lymphocytes and all monocytes and granulocytes. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chimpanzee Marmoset - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to RPE-Cy5 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.2% Bovine Serum Albumin - Immunogen
- Human IL-2 dependent T cells
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE-Cy5 488nm laser 496 667 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
- Acknowledgements
- Cy® and CyDye® are registered trademarks of GE Healthcare
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock |
Source Reference
-
Smith, S.H. et al. (1986) Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.
Immunology 58: 63-70.
References for CD45RO antibody
-
Norton, A.J. et al. (1986) Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues.
J Clin Pathol. 39 (4): 399-405. -
Terry. L.A. et al. (1987) Phenotypic heterogeneity of the CD4+ and CD8+ subsets.
Leucocyte Typing III. McMichael, A.J., Beverley, P.C.L. et al. eds. University Press. pp 225-7. -
Akbar, A.N. et al. (1988) Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells.
J Immunol. 140 (7): 2171-8. -
Beverley, P.C.L. et al. (1988) Phenotypic diversity of the CD45 antigen and its relation to function.
Immunology, Suppl. 1: 3-5. -
Merkenschlager, M. et al. (1988) Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation.
Eur J Immunol. 18 (11): 1653-61. -
Terry, L.A. et al. (1988) The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45.
Immunology. 64 (2): 331-6. -
Cavers, M. et al. (2002) Differential expression of beta1 and beta2 integrins and L-selectin on CD4+ and CD8+ T lymphocytes in human blood: comparative analysis between isolated cells, whole blood samples and cryopreserved preparations.
Clin Exp Immunol. 127: 60-5. -
Leigh, J.E. et al. (2006) Characterization of the immune status of CD8+ T cells in oral lesions of human immunodeficiency virus-infected persons with oropharyngeal Candidiasis.
Clin Vaccine Immunol. 13: 678-83.
View The Latest Product References
-
Nistala, K. et al. (2008) Interleukin-17-producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers.
Arthritis Rheum. 58: 875-87. -
Hutnick, N.A. et al. (2010) Vaccination with Ad5 vectors expands Ad5-specific CD8 T cells without altering memory phenotype or functionality.
PLoS One. 5: e14385. -
Liu Y et al. (2015) Fractionation of human spermatogenic cells using STA-PUT gravity sedimentation and their miRNA profiling.
Sci Rep. 5: 8084. -
Wilson, C.L. et al. (2015) Ubiquitin C-terminal hydrolase 1: A novel functional marker for liver myofibroblasts and a therapeutic target in chronic liver disease.
J Hepatol. 63 (6): 1421-8.
Further Reading
-
Beverley, P.C. (1987) Human T cell subsets.
Immunol Lett. 14 (4): 263-7.
- RRID
- AB_321435
- UniProt
- P08575
- Entrez Gene
- PTPRC
- GO Terms
- GO:0001915 negative regulation of T cell mediated cytotoxicity
- GO:0001960 negative regulation of cytokine-mediated signaling pathway
- GO:0002378 immunoglobulin biosynthetic process
- GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
- GO:0005887 integral to plasma membrane
- GO:0005925 focal adhesion
- GO:0006469 negative regulation of protein kinase activity
- GO:0006470 protein dephosphorylation
- GO:0007411 axon guidance
- View More GO Terms
- GO:0019901 protein kinase binding
- GO:0030890 positive regulation of B cell proliferation
- GO:0033261 regulation of S phase
- GO:0042100 B cell proliferation
- GO:0045121 membrane raft
- GO:0050852 T cell receptor signaling pathway
- GO:0050853 B cell receptor signaling pathway
- GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
- GO:0051209 release of sequestered calcium ion into cytosol
- GO:0051607 defense response to virus
MCA461C
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