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CD335 antibody | 9E2

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Mouse anti Human CD335:StarBright Violet 535

Product Type
Monoclonal Antibody
Clone
9E2
Isotype
IgG1
Specificity
CD335

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Mouse anti Human CD335 antibody, clone 9E2 recognizes human NK (natural killer) cell-specific marker CD335, otherwise known as NKp46, a 46kDa type I transmembrane glycoprotein, and the first identified member of the natural cytotoxicity receptor (NCR) family.

CD335 is uniquely expressed by all resting and activated NK cells, and considered to be the major NK cell lysis receptor for autologous pathogen-infected and tumour target cells, during natural cytotoxicity responses. Ligands of CD335 include viral haemagglutinins (HAs), and heparan sulphate proteoglycans (HSPGs) on the surface of tumour cells.

Mouse anti Human CD335 antibody, clone 9E2 mediates re-directed NK cell cytotoxicity (Yusa et al. 2002, Chen et al. 2007).

Target Species
Human
Product Form
Purified IgG conjugated to StarBright Violet 535 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
CD335-Fc fusion protein
Approx. Protein Concentrations
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 535 402 540
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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References for CD335 antibody

  1. Yusa, S. et al. (2002) SHP-1- and phosphotyrosine-independent inhibitory signaling by a killer cell Ig-like receptor cytoplasmic domain in human NK cells.
    J Immunol. 168 (10): 5047-57.
  2. Chklovskaia, E. et al. (2004) Reconstitution of dendritic and natural killer-cell subsets after allogeneic stem cell transplantation: effects of endogenous flt3 ligand.
    Blood. 103 (10): 3860-8.
  3. Chen, Y. et al. (2007) Prostaglandin D2 suppresses human NK cell function via signaling through D prostanoid receptor.
    J Immunol. 179 (5): 2766-73.

Synonyms
NKp46
UniProt
O76036
Entrez Gene
NCR1
GO Terms
GO:0004872 receptor activity
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0006968 cellular defense response
GO:0016514 SWI/SNF complex
GO:0030101 natural killer cell activation
GO:0042269 regulation of natural killer cell mediated cytotoxicity

MCA4646SBV535

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